##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921153_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2460842
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.30833755275633	31.0	28.0	31.0	10.0	34.0
2	26.894082188129104	31.0	27.0	31.0	10.0	34.0
3	26.75497289139246	31.0	26.0	31.0	10.0	34.0
4	30.643491536636645	35.0	31.0	37.0	10.0	37.0
5	30.34401111489482	35.0	30.0	37.0	10.0	37.0
6	30.67195537137289	35.0	31.0	37.0	10.0	37.0
7	30.535968176746007	35.0	31.0	37.0	8.0	37.0
8	30.21135245578546	35.0	30.0	37.0	8.0	37.0
9	31.779604298041075	37.0	32.0	39.0	2.0	39.0
10	31.14789328205549	37.0	30.0	39.0	2.0	39.0
11	31.64347446930766	37.0	31.0	39.0	2.0	39.0
12	31.333092494357622	37.0	31.0	39.0	2.0	39.0
13	31.34629326059942	37.0	31.0	39.0	2.0	39.0
14	32.439905121905426	38.0	32.0	40.0	2.0	41.0
15	32.09708140547016	38.0	31.0	40.0	2.0	41.0
16	32.03001818076902	38.0	31.0	40.0	2.0	41.0
17	32.35684290173851	38.0	32.0	40.0	2.0	41.0
18	32.40724150514336	38.0	32.0	40.0	2.0	41.0
19	32.105438707564325	38.0	31.0	40.0	2.0	41.0
20	31.982045982635213	38.0	31.0	40.0	2.0	41.0
21	31.932135423566404	38.0	31.0	40.0	2.0	41.0
22	31.17481089805847	38.0	30.0	40.0	2.0	41.0
23	31.274447120132052	38.0	30.0	40.0	2.0	41.0
24	30.9101010954787	38.0	30.0	40.0	2.0	41.0
25	30.415400501129287	38.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	202175.0
3	14.0
4	1699.0
5	9823.0
6	11867.0
7	10126.0
8	8738.0
9	7571.0
10	7243.0
11	7063.0
12	6724.0
13	6515.0
14	6482.0
15	6685.0
16	7032.0
17	7886.0
18	9136.0
19	11080.0
20	14067.0
21	17209.0
22	20955.0
23	24406.0
24	28127.0
25	31729.0
26	35832.0
27	40354.0
28	44845.0
29	52948.0
30	62612.0
31	74509.0
32	93517.0
33	118392.0
34	151650.0
35	195665.0
36	259418.0
37	343489.0
38	523259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.63005510595986	18.255170350424475	12.418475212074204	24.696299331541454
2	15.725024415908845	20.121595698571475	39.206460599919815	24.946919285599858
3	18.389610072913197	24.444778618632288	30.40862277785455	26.75698853059997
4	13.415328018025122	15.983924023984725	35.38059942305957	35.22014853493058
5	13.35505971503733	38.03461996917016	33.98886370536003	14.621456610432485
6	33.164121788424616	34.77248483740209	17.407739274430323	14.655654099742973
7	29.089144664255212	32.27857489150679	20.764660248306107	17.867620195931895
8	26.27354821720724	34.919591255208665	20.586987494320958	18.21987303326314
9	27.07153873194362	16.09602165241934	19.38639286868141	37.44604674695564
10	16.033386447027116	28.536236177537095	32.56726887923604	22.86310849619975
11	35.1454150794712	23.32123725451388	22.19036429260291	19.34298337341201
12	22.888173086535954	25.251093007434644	29.365913448604065	22.494820457425337
13	28.388666251329425	22.108620848710363	25.543362305341148	23.959350594619067
14	22.465069852167176	21.254697250376147	26.21742406569119	30.06280883176548
15	24.335501154351487	28.673351244305966	23.25633186477446	23.73481573656809
16	23.609659056727406	27.80055899803099	25.18653591944445	23.40324602579716
17	22.80609395084805	28.146715980685254	26.072185576381308	22.975004492085393
18	22.948949991322245	27.42529100972112	26.943157232433435	22.682601766523202
19	23.996774526563417	26.8768589351223	27.28650544176712	21.839861096547168
20	24.468363983968924	26.219410836380515	26.479523635921744	22.832701543728824
21	24.641659116765172	26.184440333829617	26.221102574201403	22.952797975203808
22	23.819728099881214	25.359422566823923	27.556658049817877	23.264191283476986
23	24.28678783947509	25.682669004447554	26.68793104359238	23.34261211248498
24	23.69002865213062	26.06392879640731	26.620755522139362	23.62528702932271
25	23.496095552203975	25.779195168495928	27.278547217157197	23.446162062142903
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1627.0
1	1627.0
2	1788.5
3	1950.0
4	1950.0
5	1950.0
6	2462.0
7	2974.0
8	2974.0
9	2974.0
10	3782.0
11	4590.0
12	4590.0
13	4590.0
14	7089.0
15	9588.0
16	9588.0
17	9588.0
18	16087.0
19	22586.0
20	22586.0
21	22586.0
22	34250.5
23	45915.0
24	45915.0
25	45915.0
26	66151.0
27	86387.0
28	86387.0
29	86387.0
30	112998.0
31	139609.0
32	139609.0
33	139609.0
34	166231.5
35	192854.0
36	192854.0
37	192854.0
38	220825.0
39	248796.0
40	248796.0
41	248796.0
42	271602.0
43	294408.0
44	294408.0
45	294408.0
46	311410.0
47	328412.0
48	328412.0
49	328412.0
50	325469.5
51	322527.0
52	322527.0
53	322527.0
54	297503.0
55	272479.0
56	272479.0
57	272479.0
58	238392.0
59	204305.0
60	204305.0
61	204305.0
62	170934.0
63	137563.0
64	137563.0
65	137563.0
66	107758.0
67	77953.0
68	77953.0
69	77953.0
70	58791.0
71	39629.0
72	39629.0
73	39629.0
74	28132.0
75	16635.0
76	16635.0
77	16635.0
78	11643.5
79	6652.0
80	6652.0
81	6652.0
82	4475.5
83	2299.0
84	2299.0
85	2299.0
86	1554.0
87	809.0
88	809.0
89	809.0
90	532.0
91	255.0
92	255.0
93	255.0
94	142.0
95	29.0
96	29.0
97	29.0
98	20.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03470356894103725
2	0.05607836667287051
3	0.01552314207901198
4	0.011215673334574102
5	0.009712122923779747
6	6.908204590136221E-4
7	0.0036166482854242572
8	0.0016254599035614638
9	0.00597356514558838
10	0.0033728292998900376
11	0.010768671861094698
12	0.00812729951780732
13	0.00776157103950599
14	0.014629139132053175
15	0.0062173841311226
16	0.029502097249640574
17	0.03901103768547513
18	0.02149670722460036
19	0.017392420968107666
20	0.015076140605532577
21	0.022146891186024944
22	0.006461203116656818
23	0.015807597562135237
24	0.011865857295998688
25	0.47073318807139997
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2460842.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.84289661794927
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.75809572696895	32.43756067294396
2	11.984510722365222	10.988093721241869
3	5.683541646608469	7.816500363878437
4	3.2363034029167825	5.93446089296926
5	2.0988924336097705	4.810965442308433
6	1.4137026658492589	3.888493509542812
7	1.0055779561693667	3.22690244001727
8	0.7096988297812933	2.6027720066834727
9	0.5401194499979322	2.228457609683894
>10	2.432631423074499	18.716540460112828
>50	0.09502109399989017	2.956445289170052
>100	0.03858640077786198	3.004053898245581
>500	0.002673033015075982	0.7810841604956036
>1k	6.452148657074162E-4	0.6076695327064691
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3915	0.1590918880610783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2190949276710979E-4
2	0.0	0.0	0.0	0.0	1.2190949276710979E-4
3	0.0	0.0	0.0	0.0	1.2190949276710979E-4
4	0.0	0.0	0.0	0.0	1.2190949276710979E-4
5	0.0	0.0	0.0	4.06364975890366E-5	1.2190949276710979E-4
6	0.0	0.0	0.0	4.06364975890366E-5	1.625459903561464E-4
7	0.0	4.06364975890366E-5	0.0	4.06364975890366E-5	1.625459903561464E-4
8	0.0	4.06364975890366E-5	0.0	4.06364975890366E-5	1.625459903561464E-4
9	0.0	4.06364975890366E-5	0.0	4.06364975890366E-5	1.625459903561464E-4
10	4.06364975890366E-5	4.06364975890366E-5	0.0	4.06364975890366E-5	1.625459903561464E-4
11	4.06364975890366E-5	4.06364975890366E-5	0.0	4.06364975890366E-5	1.625459903561464E-4
12	4.06364975890366E-5	4.06364975890366E-5	0.0	4.06364975890366E-5	5.282744686574758E-4
13	4.06364975890366E-5	4.06364975890366E-5	0.0	4.06364975890366E-5	6.908204590136221E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1815	0.0	13.245939	1
CTAGATC	135	3.7471182E-10	11.958356	4
TCGTCAA	70	0.0014972462	10.852521	9
TATACCG	90	9.558543E-5	10.551276	5
TAAGGTA	130	3.8642065E-7	10.22683	4
TAACGAG	75	0.0026574333	10.129225	5
ATCGGAC	105	4.122428E-5	9.948953	3
CTGTACG	105	4.1242696E-5	9.948548	4
TTTATAC	225	1.8189894E-12	9.707766	3
CCAATCG	100	2.761343E-4	9.498272	15
CGCAATG	80	0.0045298813	9.496728	18
TTAGCGC	100	2.7669853E-4	9.496342	4
CGGACCG	80	0.00453216	9.496149	5
CGATTCG	90	0.0011183461	9.495955	9
GGGACGC	220	1.6370905E-11	9.495763	6
TATGCAC	315	0.0	9.345986	3
AGGGACG	255	0.0	9.30995	5
GTATCAA	3500	0.0	9.285321	1
CTAGACA	215	1.07320375E-10	9.2754965	4
ACGCTCT	215	1.07320375E-10	9.27512	9
>>END_MODULE