FastQCFastQC Report
Mon 15 Aug 2016
SRR2921153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460842
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68480.27827873548972265No Hit
GGTATCAACGCAGAGTACTTTTTTT44630.18136068873987032No Hit
TATCAACGCAGAGTACTTTTTTTTT44560.18107623325674707No Hit
GGTATCAACGCAGAGTACATGGGGG32810.13332834858962908No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30440.1236974986610274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.013.1058971
GATCGCG604.095084E-412.6660999
TCTATAG1501.7462298E-1011.4069113
ACCGTTC752.0803256E-411.3967088
GTATCAA39850.010.5562891
GGACCGA909.590632E-510.5477896
GACGCTC2200.010.3606428
TTGCGCG1106.0961265E-610.35685318
CCTACAC2600.010.2369713
GTACTAG856.485946E-410.0772251
ACGGTCC951.6495086E-49.9971118
CGGAAAT2107.2759576E-129.95193410
ACGCTCT2107.2759576E-129.9519349
GATCTAT1151.0048521E-59.9311771
GCGTATC800.00444943559.5173781
CGGCGAG800.00451870369.49957314
TAGAGTG1502.652905E-79.4991875
TAGATAG1502.652905E-79.4991875
TACGCTG800.00452021939.49918655
ATTTGCG1106.8881985E-59.49378216
Produced by FastQC (version 0.11.5)