FastQCFastQC Report
Mon 15 Aug 2016
SRR2921152_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921152_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537357
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43590.28353856651382864No Hit
TATCAACGCAGAGTACTTTTTTTTT25420.16534871210785784No Hit
GGTATCAACGCAGAGTACTTTTTTT22130.14394834771624287No Hit
GGTATCAACGCAGAGTACATGGGGG15990.10400967374526542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT350.002175777516.2794426
TACCGTC456.782053E-414.7720857
TCTACGC400.00529143814.2449723
TAGGCCG400.005292580514.244515
GCAACGC551.9660035E-413.8133093
GTATATA1001.0015356E-813.3052641
ACGGTCC500.001503894913.2948768
GTATTAA1501.8189894E-1212.6716811
GTATTAT1155.29144E-912.3962091
GTCTAGG1251.4006218E-912.1648141
GTTATCA2600.012.0624651
CGTCACT1052.7056558E-711.76656151
GTTCTAG907.427496E-611.6157071
TACTATA752.0711802E-411.4015432
GGTATCA17300.011.2067461
GTATAAA1451.209628E-911.142341
AGCAACG600.005869690311.0848342
ACTGCGC600.005892483511.0790638
CGCAGAA951.3580162E-511.001492
ACCGTCC951.3658766E-510.9957628
Produced by FastQC (version 0.11.5)