##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921152_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1537357
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.45104422720292	31.0	27.0	31.0	7.0	34.0
2	26.209436064622597	31.0	26.0	31.0	7.0	34.0
3	26.041774291852835	31.0	26.0	31.0	7.0	34.0
4	29.785483788085656	35.0	30.0	37.0	7.0	37.0
5	29.552043539659298	35.0	30.0	37.0	2.0	37.0
6	29.85421473346789	35.0	30.0	37.0	2.0	37.0
7	29.78778839267652	35.0	30.0	37.0	2.0	37.0
8	29.40351265190844	35.0	30.0	37.0	2.0	37.0
9	30.96120289561891	37.0	30.0	39.0	2.0	39.0
10	30.304998123402697	37.0	29.0	39.0	2.0	39.0
11	30.74701712094198	37.0	30.0	39.0	2.0	39.0
12	30.446957343024426	37.0	30.0	39.0	2.0	39.0
13	30.428426188582094	37.0	30.0	39.0	2.0	39.0
14	31.470346835510554	38.0	30.0	40.0	2.0	41.0
15	31.101372680515976	38.0	29.0	40.0	2.0	41.0
16	31.033206990959158	37.0	29.0	40.0	2.0	41.0
17	31.359745979626073	38.0	30.0	40.0	2.0	41.0
18	31.413558464299445	38.0	30.0	40.0	2.0	41.0
19	31.109515226456836	38.0	30.0	40.0	2.0	41.0
20	30.97494726338775	38.0	30.0	40.0	2.0	41.0
21	30.909693064135396	38.0	30.0	40.0	2.0	41.0
22	30.162268750849673	37.0	27.0	40.0	2.0	41.0
23	30.23200661915222	37.0	27.0	40.0	2.0	41.0
24	29.852956730284507	37.0	27.0	40.0	2.0	41.0
25	29.360711272658204	37.0	25.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	152693.0
3	8.0
4	1263.0
5	6858.0
6	8393.0
7	7419.0
8	6115.0
9	5581.0
10	5211.0
11	4855.0
12	4768.0
13	4557.0
14	4654.0
15	4703.0
16	4968.0
17	5441.0
18	6259.0
19	7845.0
20	9592.0
21	12091.0
22	14426.0
23	16480.0
24	18601.0
25	21096.0
26	23409.0
27	26456.0
28	29098.0
29	34048.0
30	40258.0
31	47579.0
32	59220.0
33	74267.0
34	93942.0
35	120369.0
36	158109.0
37	208152.0
38	288573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.1938737596784	17.90403299325416	12.052975047485065	24.849118199582378
2	16.3792879028878	19.66857833094267	38.47228694228301	25.479846823886522
3	18.897232085583763	24.185519096176343	29.34701004609938	27.570238772140517
4	13.529284718029851	15.924248495951037	34.50250588088349	36.04396090513563
5	14.336278925743489	37.1088047712352	32.77739179761803	15.77752450540329
6	33.44755608200361	34.27183322958317	16.773051236968623	15.5075594514446
7	29.70809305895707	32.18118720748323	19.28589317019069	18.82482656336901
8	26.9120563676019	33.331663779639726	20.236942192895896	19.519337659862476
9	27.866742038914715	15.626709617572576	18.691840477108933	37.81470786640377
10	16.958096203295764	27.96051797071112	31.133339014263257	23.94804681172986
11	35.54427665599566	23.206025031437292	20.972854961693013	20.27684335087403
12	24.326384895409138	25.59412136651167	27.49819805520066	22.581295682878533
13	29.19569570155615	21.564816224254418	24.574338398208752	24.665149675980675
14	23.52182322185191	21.663445330862956	24.896642936557143	29.91808851072799
15	25.546410141153647	27.886676367903696	22.284439662490623	24.282473828452034
16	24.48786385025402	27.350386753560425	23.985617764637297	24.176131631548266
17	23.835131162230173	27.292728016913497	24.825851254684363	24.046289566171968
18	23.63285036726674	26.299421611809787	26.386797915460335	23.680930105463133
19	24.830933274348375	25.874902007982488	26.694814636510607	22.599350081158533
20	25.741921759026443	25.008473622475236	25.846077379624145	23.403527238874183
21	26.059384889240505	24.89746419053964	25.499797010326446	23.543353909893405
22	25.08794777255353	24.35008417486515	26.40293610256063	24.159031950020683
23	25.03543935365385	24.81971306321574	25.660303955480757	24.484543627649657
24	24.904045568921955	25.128351249164062	25.81343336013552	24.154169821778467
25	24.1725247634758	24.826413851338103	26.455548613271496	24.5455127719146
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1770.0
1	1770.0
2	1795.0
3	1820.0
4	1820.0
5	1820.0
6	2295.0
7	2770.0
8	2770.0
9	2770.0
10	3435.5
11	4101.0
12	4101.0
13	4101.0
14	5387.5
15	6674.0
16	6674.0
17	6674.0
18	10618.5
19	14563.0
20	14563.0
21	14563.0
22	20928.0
23	27293.0
24	27293.0
25	27293.0
26	38150.5
27	49008.0
28	49008.0
29	49008.0
30	61663.5
31	74319.0
32	74319.0
33	74319.0
34	86730.0
35	99141.0
36	99141.0
37	99141.0
38	113666.5
39	128192.0
40	128192.0
41	128192.0
42	143932.0
43	159672.0
44	159672.0
45	159672.0
46	176351.0
47	193030.0
48	193030.0
49	193030.0
50	197938.5
51	202847.0
52	202847.0
53	202847.0
54	193162.0
55	183477.0
56	183477.0
57	183477.0
58	167938.0
59	152399.0
60	152399.0
61	152399.0
62	130980.5
63	109562.0
64	109562.0
65	109562.0
66	87065.5
67	64569.0
68	64569.0
69	64569.0
70	48789.5
71	33010.0
72	33010.0
73	33010.0
74	24054.0
75	15098.0
76	15098.0
77	15098.0
78	11724.5
79	8351.0
80	8351.0
81	8351.0
82	5980.0
83	3609.0
84	3609.0
85	3609.0
86	2573.0
87	1537.0
88	1537.0
89	1537.0
90	1007.5
91	478.0
92	478.0
93	478.0
94	270.5
95	63.0
96	63.0
97	63.0
98	33.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03629605875538343
2	0.05366352772973356
3	0.01580634816766698
4	0.011253079148174432
5	0.009431771540377415
6	6.504670027846492E-4
7	0.003382428414480176
8	0.0013009340055692984
9	0.006374576627289562
10	0.003252335013923246
11	0.011057939047339036
12	0.008391024335921975
13	0.006699810128681887
14	0.013659807058477634
15	0.006244483226732633
16	0.02998652882837233
17	0.03876783336596509
18	0.021270270991058028
19	0.015741301467388513
20	0.01522092786516079
21	0.02166055119272882
22	0.005268782722555659
23	0.014570460862376145
24	0.010992892347060572
25	0.47360502472750315
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1537357.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.51128239616964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.84695445744629	31.98000079160131
2	10.904144563789197	9.707149159349578
3	5.336534461215691	7.126079772601878
4	3.142492214545465	5.595054335575908
5	2.0723214106834202	4.612084176327919
6	1.4374683015511025	3.839013450353007
7	1.0511105150148023	3.2750393874385026
8	0.7766327253002645	2.765513484315753
9	0.5739988201642405	2.299448122145883
>10	2.7036499356805503	19.880190937968084
>50	0.10482761142300795	3.165011108167576
>100	0.04569674463674502	3.7139267539340555
>500	0.002470067288420738	0.7301779600748437
>1k	0.0016981712607892572	1.3113105601457031
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4359	0.28353856651382864	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2542	0.16534871210785784	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2213	0.14394834771624287	No Hit
GGTATCAACGCAGAGTACATGGGGG	1599	0.10400967374526542	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	6.504670027846492E-5	0.0	0.0	0.0	0.0
6	6.504670027846492E-5	0.0	0.0	0.0	0.0
7	6.504670027846492E-5	0.0	0.0	0.0	0.0
8	6.504670027846492E-5	0.0	0.0	0.0	0.0
9	6.504670027846492E-5	0.0	0.0	0.0	0.0
10	6.504670027846492E-5	0.0	0.0	0.0	0.0
11	6.504670027846492E-5	0.0	0.0	0.0	0.0
12	6.504670027846492E-5	0.0	0.0	0.0	0.0
13	6.504670027846492E-5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	35	0.0021757775	16.279442	6
TACCGTC	45	6.782053E-4	14.772085	7
TCTACGC	40	0.005291438	14.244972	3
TAGGCCG	40	0.0052925805	14.24451	5
GCAACGC	55	1.9660035E-4	13.813309	3
GTATATA	100	1.0015356E-8	13.305264	1
ACGGTCC	50	0.0015038949	13.294876	8
GTATTAA	150	1.8189894E-12	12.671681	1
GTATTAT	115	5.29144E-9	12.396209	1
GTCTAGG	125	1.4006218E-9	12.164814	1
GTTATCA	260	0.0	12.062465	1
CGTCACT	105	2.7056558E-7	11.7665615	1
GTTCTAG	90	7.427496E-6	11.615707	1
TACTATA	75	2.0711802E-4	11.401543	2
GGTATCA	1730	0.0	11.206746	1
GTATAAA	145	1.209628E-9	11.14234	1
AGCAACG	60	0.0058696903	11.084834	2
ACTGCGC	60	0.0058924835	11.079063	8
CGCAGAA	95	1.3580162E-5	11.00149	2
ACCGTCC	95	1.3658766E-5	10.995762	8
>>END_MODULE