FastQCFastQC Report
Mon 15 Aug 2016
SRR2921152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537357
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81370.5292850001658691No Hit
TATCAACGCAGAGTACTTTTTTTTT53530.3481949865906227No Hit
GGTATCAACGCAGAGTACTTTTTTT50540.32874602320736174No Hit
ACGCAGAGTACTTTTTTTTTTTTTT38260.2488686752654068No Hit
GGTATCAACGCAGAGTACATGGGGG24360.15845376187834057No Hit
GGTCGGGAGTGGGTAATTTGCGCGC18600.12098686251794474No Hit
CCCATGTACTCTGCGTTGATACCAC18520.12046648891571704No Hit
GTATCAACGCAGAGTACATGGGGGG15800.10277378643997458No Hit
GGTATCAACGCAGAGTACATGGGGA15540.1010825722327345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT350.002148029216.3107511
TACACCG551.1296297E-515.543515
TAGTCAA707.2522707E-613.5732669
TAGATAG1153.783498E-1013.2157385
GACTTCG751.4894005E-512.6576687
GGTATCA20750.012.3804491
TACGAGA701.0881838E-412.2175294
TTCCGTT701.0938194E-412.21077215
CTTATAC2600.011.7102821
CGACCGT657.961847E-411.70303319
TCGACCG658.069107E-411.684381518
AGGACCG1552.5465852E-1111.6437055
TAAGGCG1157.084418E-811.5637715
ATACGCT1104.983849E-711.2244078
GCACTAG600.005808803311.1003711
TAGGACC1201.270273E-711.0862774
TTCGCAG600.00586969111.08483310
CCGCCCA600.00586969111.0848339
TCAGGAC2000.010.9282573
GGGTCAA700.001489702610.85861211
Produced by FastQC (version 0.11.5)