FastQCFastQC Report
Mon 15 Aug 2016
SRR2921151_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921151_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361247
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43010.1821495167595766No Hit
TATCAACGCAGAGTACTTTTTTTTT25450.10778203212116311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG1001.4437319E-712.3499452
AACCGTC853.964691E-612.2888567
GGTATCA15750.011.8251761
AGTACGG1052.734123E-711.7578663
ACGCATA1850.011.2924627
GCACCGT1105.0020935E-711.2219926
GTATCAA35700.010.913091
GTCCTAG2800.010.8598551
CTGACGT700.001496443410.8531864
GTGTAGC3900.010.7206261
CGTGCGC803.7812637E-410.68473510
CGCATAG2050.010.65428
GTACATA1701.382432E-1010.62031
ATAAGAC1909.094947E-1210.4963953
ACCGTCC1201.532464E-610.2874798
GAAGCGT1054.1279454E-59.94776
AACGCAT2107.2759576E-129.94776
CTAACAC3100.09.8030843
AAAGGCG1554.0899977E-89.802675
GTTTAGG2500.09.5023741
Produced by FastQC (version 0.11.5)