##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921151_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2361247
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.716271952913015	31.0	27.0	31.0	7.0	34.0
2	26.448829368549752	31.0	26.0	31.0	7.0	34.0
3	26.092608693626715	31.0	26.0	31.0	7.0	34.0
4	29.88808032365949	35.0	30.0	37.0	7.0	37.0
5	29.735509245750233	35.0	30.0	37.0	2.0	37.0
6	29.957638485088598	35.0	30.0	37.0	2.0	37.0
7	29.939886000913923	35.0	30.0	37.0	2.0	37.0
8	29.55707937373769	35.0	30.0	37.0	2.0	37.0
9	31.093583178718703	37.0	30.0	39.0	2.0	39.0
10	30.488336036001318	37.0	29.0	39.0	2.0	39.0
11	30.9438402674519	37.0	30.0	39.0	2.0	39.0
12	30.646765035593482	37.0	30.0	39.0	2.0	39.0
13	30.645610560860426	37.0	30.0	39.0	2.0	39.0
14	31.71204918418107	38.0	31.0	40.0	2.0	41.0
15	31.368119048960146	38.0	30.0	40.0	2.0	41.0
16	31.299157182624267	38.0	30.0	40.0	2.0	41.0
17	31.59743855683035	38.0	31.0	40.0	2.0	41.0
18	31.651695481243596	38.0	31.0	40.0	2.0	41.0
19	31.35523052014465	38.0	30.0	40.0	2.0	41.0
20	31.22788594331724	38.0	30.0	40.0	2.0	41.0
21	31.17416771731208	38.0	30.0	40.0	2.0	41.0
22	30.42637979000079	38.0	27.0	40.0	2.0	41.0
23	30.51215861788284	38.0	29.0	40.0	2.0	41.0
24	30.144114105809347	37.0	27.0	40.0	2.0	41.0
25	29.6594981380601	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	233627.0
3	31.0
4	1731.0
5	9874.0
6	12097.0
7	10564.0
8	8916.0
9	8157.0
10	7699.0
11	7091.0
12	6855.0
13	6579.0
14	6718.0
15	6812.0
16	7181.0
17	8068.0
18	9100.0
19	11179.0
20	14030.0
21	17622.0
22	21090.0
23	24437.0
24	27586.0
25	31135.0
26	34727.0
27	38825.0
28	42890.0
29	50049.0
30	59426.0
31	70351.0
32	87844.0
33	110491.0
34	141589.0
35	183506.0
36	242585.0
37	322309.0
38	478476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.08342357089962	18.317124463446582	12.373730954789707	25.225721010864095
2	15.628536863894169	19.965974071649367	39.43295769216916	24.972531372287303
3	18.51103384214229	24.431513258019386	29.946930648111948	27.110522251726376
4	13.642231739878829	16.08531498624307	35.43548643959827	34.83696683427983
5	13.478158665483555	37.11690572446903	34.813141014102044	14.59179459594537
6	33.06831608949573	35.868593910800726	16.776722301512347	14.286367698191196
7	28.802730855319304	32.144428703627234	20.384682984882463	18.668157456171
8	26.405059463433624	34.275848837815026	20.577554030812312	18.741537667939042
9	26.716937467996623	16.01763431041486	19.40002854627058	37.86539967531793
10	15.831515731607634	28.482362557545628	32.89132082823346	22.79480088261328
11	34.89766762403626	23.223674206887555	22.131247874296424	19.747410294779762
12	22.955145342237852	25.46593716010856	29.22799536477022	22.350922132883362
13	28.109252132752005	21.998878457489386	25.437414285435956	24.45445512432265
14	22.50550426151405	21.629185165983454	25.946313648450715	29.918996924051783
15	24.31959679810258	28.56338147473635	23.281860149930118	23.835161577230952
16	23.785588679234092	27.845706948431868	25.045932150471906	23.322772221862138
17	22.732185337312977	27.975062967489922	25.986794303375145	23.30595739182196
18	22.743350311262027	27.444982700134535	26.884431476605364	22.927235511998077
19	23.869118769532093	26.852822151946455	27.218663723104058	22.059395355417394
20	24.242920974906422	26.271992301185616	26.833731277661787	22.65135544624617
21	24.97140707681203	25.933315372741582	26.13422831300906	22.96104923743733
22	23.79668069123889	25.545658072063205	27.487088612312764	23.170572624385137
23	24.189268237308337	25.752723264944443	26.452383261869294	23.605625235877927
24	23.910864019429244	26.1379081625954	26.617370690184615	23.33385712779074
25	23.58588424303228	25.71123812830325	27.233657456927475	23.469220171737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1700.0
1	1700.0
2	1813.5
3	1927.0
4	1927.0
5	1927.0
6	2732.0
7	3537.0
8	3537.0
9	3537.0
10	4437.5
11	5338.0
12	5338.0
13	5338.0
14	7517.5
15	9697.0
16	9697.0
17	9697.0
18	16446.0
19	23195.0
20	23195.0
21	23195.0
22	34729.5
23	46264.0
24	46264.0
25	46264.0
26	66745.5
27	87227.0
28	87227.0
29	87227.0
30	110315.5
31	133404.0
32	133404.0
33	133404.0
34	157345.0
35	181286.0
36	181286.0
37	181286.0
38	206700.0
39	232114.0
40	232114.0
41	232114.0
42	253743.5
43	275373.0
44	275373.0
45	275373.0
46	290395.0
47	305417.0
48	305417.0
49	305417.0
50	305812.0
51	306207.0
52	306207.0
53	306207.0
54	285495.0
55	264783.0
56	264783.0
57	264783.0
58	234315.5
59	203848.0
60	203848.0
61	203848.0
62	170256.5
63	136665.0
64	136665.0
65	136665.0
66	107733.5
67	78802.0
68	78802.0
69	78802.0
70	58597.0
71	38392.0
72	38392.0
73	38392.0
74	27385.5
75	16379.0
76	16379.0
77	16379.0
78	11356.5
79	6334.0
80	6334.0
81	6334.0
82	4301.5
83	2269.0
84	2269.0
85	2269.0
86	1533.5
87	798.0
88	798.0
89	798.0
90	524.0
91	250.0
92	250.0
93	250.0
94	141.0
95	32.0
96	32.0
97	32.0
98	20.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03434625856591877
2	0.05429334584649551
3	0.015839088413876226
4	0.010799378464006519
5	0.010248821914861088
6	7.199585642671013E-4
7	0.0034727413099942535
8	0.0020328241814600505
9	0.007114884635110176
10	0.0032609887910921647
11	0.011180532998030278
12	0.008427750252303126
13	0.009020657305228974
14	0.014780325819365784
15	0.0062255240557214046
16	0.029645352646292405
17	0.038200154409936786
18	0.02265751952252348
19	0.016474345970582494
20	0.015415583376072049
21	0.022149313477158466
22	0.006479627078403911
23	0.01634729445924124
24	0.011434636020712784
25	0.4620016457405769
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2361247.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.52709168568453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.95216553262648	32.757728371874364
2	10.973261550801107	9.991613694286377
3	5.300322200759572	7.239247643929507
4	3.0714962924967883	5.5934517328296565
5	2.049223197433475	4.664758619699272
6	1.4030071114150915	3.8324900038237373
7	1.0206437319073565	3.252685853267581
8	0.7377687744592758	2.6870773310114053
9	0.5902052124671392	2.418329413922433
>10	2.764676610426103	21.019730571596877
>50	0.10233332443029992	3.1687707653172734
>100	0.03277235104707307	2.289047120558197
>500	0.0014482566342394535	0.4558300289103724
>1k	6.75853095978147E-4	0.6292388489730099
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4301	0.1821495167595766	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2545	0.10778203212116311	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2705151134125315E-4
2	0.0	0.0	0.0	0.0	1.2705151134125315E-4
3	0.0	0.0	0.0	0.0	1.2705151134125315E-4
4	0.0	0.0	0.0	4.235050378041772E-5	1.2705151134125315E-4
5	0.0	0.0	0.0	4.235050378041772E-5	1.2705151134125315E-4
6	0.0	0.0	0.0	4.235050378041772E-5	2.117525189020886E-4
7	0.0	0.0	0.0	4.235050378041772E-5	2.117525189020886E-4
8	0.0	0.0	0.0	4.235050378041772E-5	2.117525189020886E-4
9	0.0	0.0	0.0	4.235050378041772E-5	2.117525189020886E-4
10	0.0	0.0	0.0	4.235050378041772E-5	2.541030226825063E-4
11	0.0	0.0	0.0	4.235050378041772E-5	2.541030226825063E-4
12	0.0	0.0	0.0	4.235050378041772E-5	4.658555415845949E-4
13	0.0	0.0	0.0	4.235050378041772E-5	5.082060453650126E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	100	1.4437319E-7	12.349945	2
AACCGTC	85	3.964691E-6	12.288856	7
GGTATCA	1575	0.0	11.825176	1
AGTACGG	105	2.734123E-7	11.757866	3
ACGCATA	185	0.0	11.292462	7
GCACCGT	110	5.0020935E-7	11.221992	6
GTATCAA	3570	0.0	10.91309	1
GTCCTAG	280	0.0	10.859855	1
CTGACGT	70	0.0014964434	10.853186	4
GTGTAGC	390	0.0	10.720626	1
CGTGCGC	80	3.7812637E-4	10.684735	10
CGCATAG	205	0.0	10.6542	8
GTACATA	170	1.382432E-10	10.6203	1
ATAAGAC	190	9.094947E-12	10.496395	3
ACCGTCC	120	1.532464E-6	10.287479	8
GAAGCGT	105	4.1279454E-5	9.9477	6
AACGCAT	210	7.2759576E-12	9.9477	6
CTAACAC	310	0.0	9.803084	3
AAAGGCG	155	4.0899977E-8	9.80267	5
GTTTAGG	250	0.0	9.502374	1
>>END_MODULE