FastQCFastQC Report
Mon 15 Aug 2016
SRR2921151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361247
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69410.29395484673987937No Hit
GGTATCAACGCAGAGTACTTTTTTT46690.19773450215077032No Hit
TATCAACGCAGAGTACTTTTTTTTT46140.19540522444284736No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35090.1486079177654858No Hit
CTTATACACATCTCCGAGCCCACGA32080.13586041612758004No Hit
GGTATCAACGCAGAGTACATGGGGG24720.10469044534519259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17200.013.2759371
CTTATAC5750.013.2374551
TATACAC7550.012.4631673
TACGGGT550.003057815412.0969394
CGACTAA550.00306997512.0905310
CGTCTTA1751.8189894E-1211.39529815
GCCTTAG1701.0913936E-1111.1934371
TGACTCG600.00587855611.08298714
CCGACTA803.7733253E-410.6871669
TAGGGTC2050.010.6638544
ACGGTTC856.609111E-410.0565918
TTATACA8750.09.9935532
GACTAGT1054.108069E-59.9520711
CTAGGCT3550.09.9063474
GTATCAA34600.09.6794111
GTGTAGC3150.09.6654441
TCCTAGG3950.09.62505152
GTGCTAT2201.6370905E-119.51442051
GTCTTAC1002.714525E-49.51442051
TTCGTTG1002.733099E-49.50796319
Produced by FastQC (version 0.11.5)