##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921151_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2361247
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.21081487874839	31.0	30.0	33.0	25.0	34.0
2	29.475978582503227	31.0	30.0	34.0	25.0	34.0
3	29.593770156192893	31.0	30.0	34.0	25.0	34.0
4	33.25995416828481	35.0	35.0	37.0	28.0	37.0
5	32.72459933247136	35.0	33.0	37.0	26.0	37.0
6	32.640760157662456	35.0	33.0	37.0	25.0	37.0
7	32.48787822705545	35.0	33.0	37.0	23.0	37.0
8	32.418688938514265	35.0	33.0	37.0	23.0	37.0
9	33.76182542529435	38.0	34.0	39.0	22.0	39.0
10	33.62551355279647	38.0	34.0	39.0	22.0	39.0
11	33.62817739948426	38.0	34.0	39.0	22.0	39.0
12	33.503943467159516	38.0	34.0	39.0	19.0	39.0
13	33.47724570957634	38.0	34.0	39.0	18.0	39.0
14	34.39677763486836	39.0	34.0	41.0	17.0	41.0
15	34.376200372091525	39.0	34.0	41.0	17.0	41.0
16	34.35498149918242	39.0	34.0	41.0	17.0	41.0
17	34.327602321993425	39.0	34.0	41.0	17.0	41.0
18	34.279191461122025	39.0	34.0	41.0	17.0	41.0
19	34.318312738989185	39.0	34.0	41.0	18.0	41.0
20	34.25127697356524	39.0	34.0	41.0	18.0	41.0
21	34.17479450476803	39.0	34.0	41.0	16.0	41.0
22	34.08461800057343	39.0	34.0	41.0	10.0	41.0
23	33.94856192511838	39.0	34.0	41.0	9.0	41.0
24	33.855786794011806	39.0	34.0	41.0	2.0	41.0
25	33.70651143230674	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94186.0
3	3.0
4	1580.0
5	13305.0
6	14711.0
7	10943.0
8	8559.0
9	7318.0
10	6497.0
11	5859.0
12	5351.0
13	5187.0
14	4949.0
15	4910.0
16	4917.0
17	5254.0
18	5744.0
19	6829.0
20	8421.0
21	10818.0
22	13660.0
23	16422.0
24	19056.0
25	21634.0
26	24342.0
27	27284.0
28	31004.0
29	37089.0
30	46213.0
31	58537.0
32	74713.0
33	95914.0
34	125279.0
35	165315.0
36	224979.0
37	338012.0
38	816453.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.98385672504332	19.39545809298238	9.311803879557942	31.308881302416363
2	14.684825433340942	21.115749432397372	35.144904366209886	29.054520768051795
3	17.527029368686986	24.152026244088155	28.322084071409474	29.998860315815385
4	12.82081051729259	16.604032459335787	33.683404103137335	36.89175292023429
5	14.116721214769221	38.05235516006121	32.86019228788854	14.970731337281027
6	33.610976049821524	35.8672854826714	16.09144675206305	14.430291715444026
7	29.247542079214213	32.46509528016527	19.526441018795143	18.76092162182537
8	27.261030011253318	34.97368366438932	18.785208978429797	18.980077345927565
9	26.677790812415562	15.61372674411218	19.045836280243773	38.66264616322849
10	16.552453739211675	28.934628645288292	31.19899645651234	23.3139211589877
11	34.99979458474565	22.949246337784846	21.94482927451144	20.106129802958066
12	22.98094689957789	25.504448292700072	28.624288139763678	22.89031666795836
13	28.20353182090589	21.51467215562205	25.450456460004954	24.831339563467107
14	22.325876233268982	21.39828677094968	25.764495412688536	30.511341583092793
15	24.41624236468226	28.45480654371087	22.80310411142269	24.325846980184178
16	24.275123544529396	27.672958105825607	24.311420308907913	23.740498040737084
17	22.675380865016322	27.81513160235202	25.98352365996331	23.525963872668353
18	22.860827634986755	27.129198052573734	26.8916357660632	23.118338546376318
19	24.050902043298755	26.55254959162825	26.802350106984758	22.594198258088234
20	24.0800934782489	26.109620102736287	26.746459731550733	23.063826687464083
21	24.73064311048652	25.659658601419572	26.251909153517545	23.357789134576368
22	24.35629097562215	25.716512222805736	26.191617679445695	23.735579122126413
23	24.008021718782427	25.52524469630552	26.281463222494693	24.18527036241736
24	23.976601995039506	26.072909748858137	26.36557540354824	23.584912852554112
25	23.813735508800242	25.760558654336023	26.50706568608612	23.918640150777623
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1493.0
1	1493.0
2	1658.0
3	1823.0
4	1823.0
5	1823.0
6	2158.0
7	2493.0
8	2493.0
9	2493.0
10	3247.5
11	4002.0
12	4002.0
13	4002.0
14	5394.5
15	6787.0
16	6787.0
17	6787.0
18	13209.0
19	19631.0
20	19631.0
21	19631.0
22	30702.0
23	41773.0
24	41773.0
25	41773.0
26	61387.0
27	81001.0
28	81001.0
29	81001.0
30	101800.0
31	122599.0
32	122599.0
33	122599.0
34	145219.0
35	167839.0
36	167839.0
37	167839.0
38	193047.5
39	218256.0
40	218256.0
41	218256.0
42	240941.5
43	263627.0
44	263627.0
45	263627.0
46	283135.5
47	302644.0
48	302644.0
49	302644.0
50	309830.0
51	317016.0
52	317016.0
53	317016.0
54	297467.5
55	277919.0
56	277919.0
57	277919.0
58	249010.0
59	220101.0
60	220101.0
61	220101.0
62	186089.5
63	152078.0
64	152078.0
65	152078.0
66	119884.5
67	87691.0
68	87691.0
69	87691.0
70	65185.0
71	42679.0
72	42679.0
73	42679.0
74	30871.0
75	19063.0
76	19063.0
77	19063.0
78	12994.0
79	6925.0
80	6925.0
81	6925.0
82	4768.0
83	2611.0
84	2611.0
85	2611.0
86	1724.0
87	837.0
88	837.0
89	837.0
90	569.5
91	302.0
92	302.0
93	302.0
94	177.5
95	53.0
96	53.0
97	53.0
98	28.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10037069395959
2	0.0
3	0.03997887556871433
4	0.05285342871796132
5	0.06589738388232998
6	0.0075807401766947715
7	0.004446802896943861
8	0.007030183627549341
9	7.199585642671013E-4
10	5.92907052925848E-4
11	0.007453688665353519
12	1.694020151216709E-4
13	0.006013771536819316
14	0.05331928425954591
15	0.021471705416671785
16	0.006564328085964746
17	0.0018210716625579621
18	0.007707791688036024
19	0.0069031321162080885
20	0.002371628211703392
21	0.010714677456445684
22	0.0036844938288963414
23	0.006649029093525582
24	0.008088946222059785
25	0.16381174862265574
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2361247.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.45872791120164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.70546945550745	20.0288104368928
2	12.81006144091641	8.315965976729627
3	6.696413665158489	6.5207120741469575
4	4.338474688239056	5.632854778207475
5	2.991580983007871	4.8551456575888805
6	2.094508818864931	4.079105511548947
7	1.5813957760044306	3.593106664927598
8	1.2246284204135678	3.1799904552422924
9	0.9776257000346962	2.855923793678181
>10	5.271307325322989	29.63991056815561
>50	0.22059289210201108	4.854599559543053
>100	0.08344059106771246	4.311607433383656
>500	0.0023824817789910523	0.518942882060883
>1k	0.0019854014824925354	1.3151221593480948
>5k	1.323600988328357E-4	0.2982020485457883
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6941	0.29395484673987937	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4669	0.19773450215077032	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4614	0.19540522444284736	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3509	0.1486079177654858	No Hit
CTTATACACATCTCCGAGCCCACGA	3208	0.13586041612758004	No Hit
GGTATCAACGCAGAGTACATGGGGG	2472	0.10469044534519259	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.470100756083544E-5
2	0.0	0.0	0.0	0.0	8.470100756083544E-5
3	0.0	0.0	0.0	0.0	8.470100756083544E-5
4	0.0	0.0	0.0	4.235050378041772E-5	8.470100756083544E-5
5	0.0	0.0	0.0	4.235050378041772E-5	8.470100756083544E-5
6	0.0	0.0	0.0	4.235050378041772E-5	1.2705151134125315E-4
7	0.0	0.0	0.0	4.235050378041772E-5	1.2705151134125315E-4
8	0.0	0.0	0.0	4.235050378041772E-5	1.2705151134125315E-4
9	0.0	0.0	0.0	4.235050378041772E-5	1.2705151134125315E-4
10	0.0	0.0	0.0	4.235050378041772E-5	1.2705151134125315E-4
11	0.0	0.0	0.0	4.235050378041772E-5	1.2705151134125315E-4
12	0.0	0.0	0.0	4.235050378041772E-5	5.082060453650126E-4
13	0.0	0.0	0.0	4.235050378041772E-5	5.505565491454303E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1720	0.0	13.275937	1
CTTATAC	575	0.0	13.237455	1
TATACAC	755	0.0	12.463167	3
TACGGGT	55	0.0030578154	12.096939	4
CGACTAA	55	0.003069975	12.09053	10
CGTCTTA	175	1.8189894E-12	11.395298	15
GCCTTAG	170	1.0913936E-11	11.193437	1
TGACTCG	60	0.005878556	11.082987	14
CCGACTA	80	3.7733253E-4	10.687166	9
TAGGGTC	205	0.0	10.663854	4
ACGGTTC	85	6.609111E-4	10.056591	8
TTATACA	875	0.0	9.993553	2
GACTAGT	105	4.108069E-5	9.95207	11
CTAGGCT	355	0.0	9.906347	4
GTATCAA	3460	0.0	9.679411	1
GTGTAGC	315	0.0	9.665444	1
TCCTAGG	395	0.0	9.6250515	2
GTGCTAT	220	1.6370905E-11	9.5144205	1
GTCTTAC	100	2.714525E-4	9.5144205	1
TTCGTTG	100	2.733099E-4	9.507963	19
>>END_MODULE