FastQCFastQC Report
Mon 15 Aug 2016
SRR2921150_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921150_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909797
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30160.10364984223985385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.012.6131421
TCTACGC1157.1197064E-811.5607713
GTCCTAG3100.011.0344651
CGTCCCA1002.409638E-510.446341510
GTATCAA34100.010.2821151
CAACGCG750.002657220510.1294374
GATCGTG750.002657599310.1292647
GCGCCAC2107.2759576E-129.95077913
GGACCGT1054.1257204E-59.9483846
GTACCGT1356.483624E-79.8478956
TACACCG1701.6772901E-89.4961845
GTGCGCC1752.6790076E-89.22708611
GTTTAGG3400.09.2224321
TATTAGG1554.2608008E-79.1939642
TAATACC2700.09.1446314
ACCGTCC1252.7681035E-59.1164948
ACCGGTC950.00183084728.996548
TAGGACC1901.0657459E-88.996544
AGTCGCG1606.772825E-78.90374418
AAGACGG1606.784576E-78.9026745
Produced by FastQC (version 0.11.5)