##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921150_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2909797
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.958871014026066	31.0	27.0	31.0	10.0	34.0
2	26.77143079053281	31.0	27.0	31.0	10.0	34.0
3	26.5086347947984	31.0	26.0	31.0	10.0	34.0
4	30.4614180989258	35.0	31.0	37.0	10.0	37.0
5	30.011338935327792	35.0	30.0	37.0	10.0	37.0
6	30.461796132170043	35.0	31.0	37.0	8.0	37.0
7	30.364777336700808	35.0	31.0	37.0	8.0	37.0
8	30.109225145259273	35.0	30.0	37.0	2.0	37.0
9	31.67868652005621	37.0	31.0	39.0	2.0	39.0
10	31.065501476563487	37.0	30.0	39.0	2.0	39.0
11	31.498053644292025	37.0	31.0	39.0	2.0	39.0
12	31.193858540647337	37.0	30.0	39.0	2.0	39.0
13	31.19739418248077	37.0	30.0	39.0	2.0	39.0
14	32.28559346236181	38.0	31.0	40.0	2.0	41.0
15	31.932051617346502	38.0	31.0	40.0	2.0	41.0
16	31.857778394850225	38.0	31.0	40.0	2.0	41.0
17	32.1716906712049	38.0	31.0	40.0	2.0	41.0
18	32.223447546340864	38.0	32.0	40.0	2.0	41.0
19	31.929895796854556	38.0	31.0	40.0	2.0	41.0
20	31.80137686580885	38.0	31.0	40.0	2.0	41.0
21	31.74935261806923	38.0	31.0	40.0	2.0	41.0
22	30.986895305755006	38.0	29.0	40.0	2.0	41.0
23	31.089706257859227	38.0	30.0	40.0	2.0	41.0
24	30.719079372203627	38.0	30.0	40.0	2.0	41.0
25	30.224034528869197	37.0	28.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	247748.0
3	8.0
4	2208.0
5	11710.0
6	13640.0
7	11824.0
8	10302.0
9	9352.0
10	8695.0
11	8270.0
12	8071.0
13	7945.0
14	7885.0
15	7996.0
16	8481.0
17	9424.0
18	10892.0
19	13205.0
20	16489.0
21	20854.0
22	25579.0
23	29785.0
24	34285.0
25	38466.0
26	43357.0
27	48910.0
28	54500.0
29	63307.0
30	75319.0
31	89697.0
32	111965.0
33	141256.0
34	181588.0
35	234436.0
36	308322.0
37	408353.0
38	585673.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.51836893534982	17.962619945379988	12.35213955880452	25.16687156046567
2	15.674233530912185	19.497208932242078	39.2826711725373	25.545886364308444
3	19.262695144669998	23.347895427891853	29.71880606864693	27.67060335879122
4	13.39179258502604	15.418366992714533	35.17543102900129	36.014409393258134
5	13.708335610334183	36.86589822613901	34.402750410805325	15.023015752721488
6	34.0379919017877	34.322412225262546	17.258258190143717	14.381337682806041
7	29.30271689763716	31.46089310882747	20.692041974389618	18.544348019145755
8	26.27264210405487	34.84480024634554	20.35546891915206	18.527088730447524
9	26.76727111131274	15.795743282697734	19.136874903982076	38.30011070200745
10	15.584213840109317	28.246430545130615	33.057255623283325	23.11209999147675
11	35.67656446930247	22.684577183510164	22.074575674660363	19.564282672527007
12	23.142368599685515	24.68753490690061	29.63903042592863	22.53106606748524
13	28.531539962969976	21.216993391718457	25.31438915756526	24.937077487746308
14	22.44815607022445	20.821817329396154	25.98553563553734	30.744490964842058
15	24.367832762921562	28.180529654652847	23.104941789129345	24.34669579329625
16	24.177533592560494	27.339275321315288	24.9378889567648	23.54530212935942
17	23.085803713184962	27.476379173654415	25.857421728616114	23.58039538454451
18	23.27465511320688	27.16129801800736	26.422454997915196	23.141591870870563
19	24.274424289754563	26.50807279886817	26.72293725311727	22.494565658259994
20	24.599915512990478	25.949473468569835	26.319765801184676	23.13084521725501
21	24.995161792079088	25.72451948775151	25.942144314375238	23.338174405794163
22	23.82915835365403	25.12597114031334	27.388382231891377	23.656488274141246
23	24.2928617290842	25.442747682285834	26.30476686744586	23.959623721184105
24	23.92104511526026	25.837277651962097	26.391676253214087	23.850000979563553
25	23.73217230606038	25.472124848414534	26.959975912067996	23.83572693345709
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1175.0
1	1175.0
2	1267.5
3	1360.0
4	1360.0
5	1360.0
6	1756.5
7	2153.0
8	2153.0
9	2153.0
10	3040.5
11	3928.0
12	3928.0
13	3928.0
14	6367.0
15	8806.0
16	8806.0
17	8806.0
18	15870.0
19	22934.0
20	22934.0
21	22934.0
22	36301.5
23	49669.0
24	49669.0
25	49669.0
26	72669.5
27	95670.0
28	95670.0
29	95670.0
30	122937.5
31	150205.0
32	150205.0
33	150205.0
34	181477.0
35	212749.0
36	212749.0
37	212749.0
38	247288.0
39	281827.0
40	281827.0
41	281827.0
42	313143.5
43	344460.0
44	344460.0
45	344460.0
46	366481.0
47	388502.0
48	388502.0
49	388502.0
50	389717.0
51	390932.0
52	390932.0
53	390932.0
54	366789.5
55	342647.0
56	342647.0
57	342647.0
58	301112.0
59	259577.0
60	259577.0
61	259577.0
62	217524.5
63	175472.0
64	175472.0
65	175472.0
66	137332.5
67	99193.0
68	99193.0
69	99193.0
70	73348.5
71	47504.0
72	47504.0
73	47504.0
74	33461.5
75	19419.0
76	19419.0
77	19419.0
78	13512.5
79	7606.0
80	7606.0
81	7606.0
82	5168.0
83	2730.0
84	2730.0
85	2730.0
86	1855.0
87	980.0
88	980.0
89	980.0
90	620.5
91	261.0
92	261.0
93	261.0
94	145.0
95	29.0
96	29.0
97	29.0
98	19.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03343875878626584
2	0.054677353781036955
3	0.014330896622685363
4	0.010516197521682783
5	0.009210264496114333
6	6.529665127842251E-4
7	0.004055265710975714
8	0.0021650994897582203
9	0.006323465176436707
10	0.003745965783867397
11	0.010172530936006877
12	0.009347731130384698
13	0.009141531178979152
14	0.01512132976973995
15	0.006976431689220932
16	0.030723792759426174
17	0.039006157474215555
18	0.02247579470320438
19	0.017905029113714804
20	0.01628979616103804
21	0.022750727971745106
22	0.006942065030653341
23	0.015980496233929723
24	0.011615930595845689
25	0.4722322553772651
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2909797.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.98693200525618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.36236416031244	33.061116205296756
2	12.01034396553808	11.286584305369532
3	5.811486126770564	8.191917104641744
4	3.3310789912932104	6.260687282721259
5	2.1785219911110567	5.1181032334145256
6	1.4481086652534134	4.082531003429065
7	0.9835077189354504	3.2348407221385225
8	0.7286274101185959	2.7388773261126698
9	0.5471583597876771	2.313836338270597
>10	2.504641395143577	19.10919264468908
>50	0.07125829079395793	2.2870578381646065
>100	0.02154494766724076	1.6083432731788108
>500	9.053181831580092E-4	0.3411912022302831
>1k	4.5265909155662264E-4	0.3657215203425687
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3016	0.10364984223985385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.87333171351816E-5	0.0	0.0	0.0
3	0.0	1.030999757027724E-4	0.0	0.0	0.0
4	0.0	2.061999514055448E-4	0.0	0.0	0.0
5	0.0	2.061999514055448E-4	0.0	0.0	0.0
6	0.0	2.061999514055448E-4	0.0	0.0	0.0
7	0.0	2.061999514055448E-4	0.0	0.0	0.0
8	0.0	2.061999514055448E-4	0.0	0.0	0.0
9	0.0	4.467665613786804E-4	0.0	0.0	0.0
10	0.0	4.467665613786804E-4	0.0	0.0	0.0
11	0.0	4.467665613786804E-4	0.0	3.43666585675908E-5	0.0
12	0.0	4.467665613786804E-4	0.0	3.43666585675908E-5	3.43666585675908E-5
13	0.0	4.467665613786804E-4	0.0	3.43666585675908E-5	3.43666585675908E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1695	0.0	12.613142	1
TCTACGC	115	7.1197064E-8	11.560771	3
GTCCTAG	310	0.0	11.034465	1
CGTCCCA	100	2.409638E-5	10.4463415	10
GTATCAA	3410	0.0	10.282115	1
CAACGCG	75	0.0026572205	10.129437	4
GATCGTG	75	0.0026575993	10.129264	7
GCGCCAC	210	7.2759576E-12	9.950779	13
GGACCGT	105	4.1257204E-5	9.948384	6
GTACCGT	135	6.483624E-7	9.847895	6
TACACCG	170	1.6772901E-8	9.496184	5
GTGCGCC	175	2.6790076E-8	9.227086	11
GTTTAGG	340	0.0	9.222432	1
TATTAGG	155	4.2608008E-7	9.193964	2
TAATACC	270	0.0	9.144631	4
ACCGTCC	125	2.7681035E-5	9.116494	8
ACCGGTC	95	0.0018308472	8.99654	8
TAGGACC	190	1.0657459E-8	8.99654	4
AGTCGCG	160	6.772825E-7	8.903744	18
AAGACGG	160	6.784576E-7	8.902674	5
>>END_MODULE