FastQCFastQC Report
Mon 15 Aug 2016
SRR2921150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909797
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55950.1922814546856705No Hit
TATCAACGCAGAGTACTTTTTTTTT35480.12193290459781216No Hit
GGTATCAACGCAGAGTACTTTTTTT35130.12073007154794646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17400.013.3984621
CCGTCCG550.003071695312.0897829
CCGCCTA600.005881824511.082314
TGTGCGA1053.4728728E-610.85613110
CTCTTAC1855.456968E-1210.801541
CTCGTAG1303.7590144E-710.2476161
CCCGCGA750.002662932310.12681618
GTATCAA36050.010.083151
CCGCTCA1054.1117604E-59.9514539
CCGATCA1151.0282629E-59.912129
GTATAAT2850.09.6825841
CGTACAC1401.0495751E-69.5064723
CTTACAC3800.09.5064723
TGGACGG1201.7033337E-59.4991155
TGCTACG900.00111507769.49911510
TACCGTA1106.847172E-59.49911412
ACCGACC900.00111613049.4981358
TATTAGG1951.6643753E-99.2627162
GTAGGAC1651.055414E-79.2183963
TATTAAG2501.8189894E-129.1262122
Produced by FastQC (version 0.11.5)