##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921150_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2909797
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.67944705421031	31.0	30.0	33.0	26.0	34.0
2	29.93870328411226	31.0	30.0	34.0	26.0	34.0
3	30.053054903830063	31.0	30.0	34.0	26.0	34.0
4	33.744005853329284	35.0	35.0	37.0	30.0	37.0
5	33.28020820696427	35.0	35.0	37.0	27.0	37.0
6	33.22595906174898	36.0	35.0	37.0	27.0	37.0
7	33.09256934418449	36.0	35.0	37.0	26.0	37.0
8	33.03536019866678	37.0	35.0	37.0	26.0	37.0
9	34.45530667603273	39.0	35.0	39.0	27.0	39.0
10	34.32482128478378	38.0	35.0	39.0	26.0	39.0
11	34.335496599934636	38.0	35.0	39.0	27.0	39.0
12	34.21014730580862	38.0	35.0	39.0	26.0	39.0
13	34.19507168369477	38.0	35.0	39.0	26.0	39.0
14	35.16075932444772	39.0	35.0	41.0	25.0	41.0
15	35.149305948146896	39.0	35.0	41.0	25.0	41.0
16	35.13725081165456	39.0	35.0	41.0	25.0	41.0
17	35.118739554683714	39.0	35.0	41.0	25.0	41.0
18	35.0753571469075	39.0	35.0	41.0	25.0	41.0
19	35.115383306808	39.0	35.0	41.0	25.0	41.0
20	35.0511248722849	39.0	35.0	41.0	25.0	41.0
21	34.98124508341991	39.0	35.0	41.0	24.0	41.0
22	34.900634305417185	39.0	35.0	41.0	24.0	41.0
23	34.776872063583816	39.0	35.0	41.0	23.0	41.0
24	34.69285795538314	39.0	35.0	41.0	22.0	41.0
25	34.55425309738102	39.0	35.0	41.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91089.0
3	4.0
4	1431.0
5	12546.0
6	14239.0
7	10458.0
8	8265.0
9	6981.0
10	6400.0
11	5805.0
12	5482.0
13	5064.0
14	5025.0
15	5028.0
16	5006.0
17	5331.0
18	5892.0
19	7110.0
20	8838.0
21	11222.0
22	14192.0
23	17530.0
24	20897.0
25	24021.0
26	27516.0
27	31396.0
28	35847.0
29	43352.0
30	54800.0
31	70985.0
32	90038.0
33	117582.0
34	155663.0
35	205899.0
36	282071.0
37	428923.0
38	1067869.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.29136684336418	19.03252007068818	9.308182634748048	31.367930451199598
2	14.720477064207572	20.759420674363195	35.53866472472135	28.981437536707887
3	17.984117291921947	23.62936176240437	28.156978751084726	30.22954219458896
4	13.096787948186279	16.0916540295931	33.56547723435496	37.24608078786566
5	14.27815772141581	37.71914589205168	33.03197315994834	14.97072322658417
6	34.32453324616768	35.22977050618288	16.19019434571285	14.255501901936581
7	29.74380212155008	31.955500031790603	19.5039291464825	18.796768700176823
8	27.651759785551537	35.16738920546625	18.517489930113147	18.663361078869066
9	26.932189421750497	15.567901650364185	18.788417826490093	38.711491101395225
10	16.350887539191014	28.817229577285207	31.30655267894179	23.525330204581984
11	35.95519019111744	22.57604155961288	21.708257099578155	19.76051114969153
12	23.340174596552195	24.829524014414766	29.04576750106709	22.784533887965953
13	28.68152285687576	20.92886818184318	25.16053841373887	25.229070547542193
14	22.50539050651847	20.604871573546454	25.794854688451075	31.094883231484
15	24.470770295832402	28.239872321454047	22.637092707535214	24.65226467517834
16	24.594580288947686	27.248858376863836	24.250804446470024	23.90575688771845
17	23.169404803963484	27.433049553159638	25.78721160891399	23.610334033962893
18	23.395876599070718	27.002082254912274	26.371906263469526	23.23013488254749
19	24.473058216304857	26.352811862750592	26.269291697442533	22.904838223502026
20	24.609664303224445	25.89651511699159	26.133787257207608	23.360033322576353
21	24.90794302505132	25.47070058176708	26.01767988922913	23.60367650395247
22	24.497962154744265	25.322517064526785	25.994444083581385	24.18507669714757
23	24.309273772510082	25.376958171820746	26.01707387658388	24.296694179085293
24	23.97528474242566	25.872135389455043	26.062971935478547	24.08960793264075
25	24.036506127396123	25.497784220620694	26.209622714923714	24.25608693705947
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	733.0
1	733.0
2	822.0
3	911.0
4	911.0
5	911.0
6	1258.5
7	1606.0
8	1606.0
9	1606.0
10	2323.5
11	3041.0
12	3041.0
13	3041.0
14	4823.5
15	6606.0
16	6606.0
17	6606.0
18	12862.5
19	19119.0
20	19119.0
21	19119.0
22	31691.5
23	44264.0
24	44264.0
25	44264.0
26	66566.0
27	88868.0
28	88868.0
29	88868.0
30	113842.5
31	138817.0
32	138817.0
33	138817.0
34	168623.0
35	198429.0
36	198429.0
37	198429.0
38	232499.0
39	266569.0
40	266569.0
41	266569.0
42	299301.5
43	332034.0
44	332034.0
45	332034.0
46	357858.0
47	383682.0
48	383682.0
49	383682.0
50	393654.5
51	403627.0
52	403627.0
53	403627.0
54	380760.5
55	357894.0
56	357894.0
57	357894.0
58	316569.0
59	275244.0
60	275244.0
61	275244.0
62	233072.0
63	190900.0
64	190900.0
65	190900.0
66	150506.5
67	110113.0
68	110113.0
69	110113.0
70	81425.5
71	52738.0
72	52738.0
73	52738.0
74	37175.5
75	21613.0
76	21613.0
77	21613.0
78	15034.5
79	8456.0
80	8456.0
81	8456.0
82	5751.5
83	3047.0
84	3047.0
85	3047.0
86	2099.5
87	1152.0
88	1152.0
89	1152.0
90	720.0
91	288.0
92	288.0
93	288.0
94	165.5
95	43.0
96	43.0
97	43.0
98	23.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10124417614012249
2	0.0
3	0.042099156745298724
4	0.05797655300352568
5	0.07567538216583494
6	0.013540463475630773
7	0.004536398930921985
8	0.013059330255684502
9	5.154998785138619E-4
10	3.43666585675908E-4
11	0.014915129818334408
12	1.0309997570277238E-4
13	0.00732009827489684
14	0.06553721788839564
15	0.02116986167763593
16	0.011787763888683644
17	0.0011340997327304962
18	0.015499363013983448
19	0.010997330741629056
20	0.001993266196920266
21	0.025053294095773688
22	0.003642865808164625
23	0.011100430717331827
24	0.017217695942362988
25	0.19338118775983343
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2909797.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.296298869753265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.08677355853003	20.26457645212781
2	14.553052740006562	9.982316924770933
3	7.669864951897644	7.891439420427821
4	4.59887960372078	6.308981974208814
5	3.1097929204553796	5.332719371149027
6	2.2276450041942577	4.583998730373541
7	1.6300909792294185	3.9134261185978625
8	1.2403541106150917	3.403164422558574
9	0.9268909223278746	2.861003528263625
>10	4.739764956990773	27.346419419328864
>50	0.1581598521718461	3.6661261047096216
>100	0.056102921239289755	3.1619975167665992
>500	0.0012126824405230936	0.2664219356113268
>1k	0.0013137393104812386	0.8234922843492195
>5k	1.0105687003701836E-4	0.19391579675647658
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5595	0.1922814546856705	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3548	0.12193290459781216	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3513	0.12073007154794646	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.030999757027724E-4	0.0	0.0	0.0
5	0.0	1.374666342703632E-4	0.0	0.0	0.0
6	0.0	1.374666342703632E-4	0.0	0.0	0.0
7	0.0	1.7183329283795398E-4	0.0	0.0	0.0
8	0.0	2.061999514055448E-4	0.0	0.0	0.0
9	0.0	2.749332685407264E-4	0.0	0.0	0.0
10	0.0	3.0929992710831717E-4	0.0	0.0	0.0
11	0.0	3.0929992710831717E-4	0.0	0.0	0.0
12	0.0	3.0929992710831717E-4	0.0	0.0	0.0
13	0.0	3.0929992710831717E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1740	0.0	13.398462	1
CCGTCCG	55	0.0030716953	12.089782	9
CCGCCTA	60	0.0058818245	11.0823	14
TGTGCGA	105	3.4728728E-6	10.856131	10
CTCTTAC	185	5.456968E-12	10.80154	1
CTCGTAG	130	3.7590144E-7	10.247616	1
CCCGCGA	75	0.0026629323	10.126816	18
GTATCAA	3605	0.0	10.08315	1
CCGCTCA	105	4.1117604E-5	9.951453	9
CCGATCA	115	1.0282629E-5	9.91212	9
GTATAAT	285	0.0	9.682584	1
CGTACAC	140	1.0495751E-6	9.506472	3
CTTACAC	380	0.0	9.506472	3
TGGACGG	120	1.7033337E-5	9.499115	5
TGCTACG	90	0.0011150776	9.499115	10
TACCGTA	110	6.847172E-5	9.499114	12
ACCGACC	90	0.0011161304	9.498135	8
TATTAGG	195	1.6643753E-9	9.262716	2
GTAGGAC	165	1.055414E-7	9.218396	3
TATTAAG	250	1.8189894E-12	9.126212	2
>>END_MODULE