FastQCFastQC Report
Mon 15 Aug 2016
SRR2921149_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921149_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2368502
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13900.013.3990961
GTATAGG2850.011.0028021
GTATCAA29050.010.2711181
TCGCGCA1054.1209456E-59.9492418
TACGCTC1054.1295592E-59.9473494
GTATTAA2800.09.8417921
GTATTGA2106.730261E-119.5024191
GTCTAGG2800.09.5024191
ATTCGCC900.00111524739.49880611
CGTGCGC1201.7056931E-59.49780410
CGTACAC1106.873514E-59.4955973
GTCTAGA2950.09.3413611
GTCCTAC1252.7466473E-59.1223221
GTTCGCG1151.1119497E-49.086216
TGTACGC950.00182417429.000012
GACTTAG1901.0670192E-88.9956397
GTATTAG1804.202775E-88.9745071
TCGTGCG850.00746290068.937419
TACCGTA850.0074667798.9368447
TAGGGAC1701.6986087E-78.9366564
Produced by FastQC (version 0.11.5)