##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921149_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2368502
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.95382566702498	31.0	27.0	31.0	10.0	34.0
2	26.821748936669675	31.0	27.0	31.0	10.0	34.0
3	26.57799148997974	31.0	26.0	31.0	10.0	34.0
4	30.49034832987264	35.0	31.0	37.0	10.0	37.0
5	30.13068555568034	35.0	30.0	37.0	10.0	37.0
6	30.512010545061816	35.0	31.0	37.0	10.0	37.0
7	30.478857522602894	35.0	31.0	37.0	8.0	37.0
8	30.0540670854405	35.0	30.0	37.0	7.0	37.0
9	31.758408057075737	37.0	32.0	39.0	2.0	39.0
10	31.11381793217823	37.0	30.0	39.0	2.0	39.0
11	31.540150694405156	37.0	31.0	39.0	2.0	39.0
12	31.279680996680604	37.0	30.0	39.0	2.0	39.0
13	31.28556446226349	37.0	30.0	39.0	2.0	39.0
14	32.39007989015842	38.0	32.0	40.0	2.0	41.0
15	32.04913907609113	38.0	31.0	40.0	2.0	41.0
16	31.976780260265773	38.0	31.0	40.0	2.0	41.0
17	32.28223113174487	38.0	31.0	40.0	2.0	41.0
18	32.33657054121128	38.0	32.0	40.0	2.0	41.0
19	32.05737761673834	38.0	31.0	40.0	2.0	41.0
20	31.92311342781218	38.0	31.0	40.0	2.0	41.0
21	31.89017277587268	38.0	31.0	40.0	2.0	41.0
22	31.138522576717268	38.0	30.0	40.0	2.0	41.0
23	31.236097330717897	38.0	30.0	40.0	2.0	41.0
24	30.870030086527265	38.0	30.0	40.0	2.0	41.0
25	30.387522999769473	38.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	198440.0
3	8.0
4	1699.0
5	9269.0
6	10935.0
7	9453.0
8	8240.0
9	7400.0
10	7024.0
11	6677.0
12	6578.0
13	6391.0
14	6144.0
15	6570.0
16	6717.0
17	7402.0
18	8576.0
19	10641.0
20	13538.0
21	16460.0
22	20244.0
23	23793.0
24	27614.0
25	30922.0
26	35353.0
27	39187.0
28	43488.0
29	51359.0
30	60764.0
31	72168.0
32	90870.0
33	114633.0
34	148594.0
35	192838.0
36	254667.0
37	337041.0
38	476805.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.12801452886767	17.91041939434895	12.732694175782406	25.22887190100097
2	15.716327827191867	19.478848441428536	39.45400152748114	25.350822203898453
3	19.197711608250575	23.11333940846053	29.26119640593448	28.427752577354415
4	13.526280386901885	15.35940688669139	35.59494799744453	35.5193647289622
5	14.030429666074253	36.52619769360139	34.03059012000688	15.412782520317478
6	33.81624501790178	34.039299466324394	17.340826183881646	14.803629331892184
7	29.61587455598017	31.796780890543435	20.0231462422823	18.56419831119409
8	26.543213264703386	34.08324681405695	20.249817285643655	19.123722635596007
9	27.08385233476754	15.969987290622505	18.937099137366836	38.00906123724312
10	16.015186611056738	27.970631674859252	32.53265281822934	23.481528895854673
11	35.764085210312764	22.630415955335064	21.814061331528315	19.79143750282386
12	23.725228074412684	24.398831907701116	29.017956294499108	22.857983723387097
13	28.54453199320534	21.228409373520847	25.301657580802683	24.925401052471127
14	22.77986897656628	20.446082683100478	25.736130594402496	31.03791774593075
15	24.57731281448463	27.8622033230604	22.9493923851916	24.611091477263372
16	24.22037303501569	27.17788532402048	24.65149653661023	23.950245104353595
17	23.392628459633247	27.135967683621164	25.569819462137055	23.90158439460853
18	23.372049909247465	26.930486685264814	26.312026547543937	23.38543685794378
19	24.617017844489695	26.03172297419985	26.477771875314065	22.873487305996388
20	24.88045470284696	25.665121149932013	26.140684250086565	23.31373989713446
21	25.414523996846228	25.370435358412337	25.650381320268906	23.56465932447253
22	24.245907003747295	24.848345384493587	26.918963424288805	23.986784187470313
23	24.649859825693177	25.17474810218895	25.919979663218506	24.255412408899367
24	24.14586802003021	25.5639777166255	26.067646501195203	24.222507762149085
25	23.94217252206432	25.324315588272974	26.639818174568365	24.093693715094343
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	835.0
1	835.0
2	906.0
3	977.0
4	977.0
5	977.0
6	1293.0
7	1609.0
8	1609.0
9	1609.0
10	2086.0
11	2563.0
12	2563.0
13	2563.0
14	3667.5
15	4772.0
16	4772.0
17	4772.0
18	8773.5
19	12775.0
20	12775.0
21	12775.0
22	22182.0
23	31589.0
24	31589.0
25	31589.0
26	50487.5
27	69386.0
28	69386.0
29	69386.0
30	94394.0
31	119402.0
32	119402.0
33	119402.0
34	146086.0
35	172770.0
36	172770.0
37	172770.0
38	201077.5
39	229385.0
40	229385.0
41	229385.0
42	253650.5
43	277916.0
44	277916.0
45	277916.0
46	297835.0
47	317754.0
48	317754.0
49	317754.0
50	322622.0
51	327490.0
52	327490.0
53	327490.0
54	307889.5
55	288289.0
56	288289.0
57	288289.0
58	253649.0
59	219009.0
60	219009.0
61	219009.0
62	182190.0
63	145371.0
64	145371.0
65	145371.0
66	113060.0
67	80749.0
68	80749.0
69	80749.0
70	59980.5
71	39212.0
72	39212.0
73	39212.0
74	27868.5
75	16525.0
76	16525.0
77	16525.0
78	11574.0
79	6623.0
80	6623.0
81	6623.0
82	4515.0
83	2407.0
84	2407.0
85	2407.0
86	1617.0
87	827.0
88	827.0
89	827.0
90	531.0
91	235.0
92	235.0
93	235.0
94	130.0
95	25.0
96	25.0
97	25.0
98	16.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033861064926269856
2	0.05091825972703422
3	0.015875012982889607
4	0.010681857140082634
5	0.009330792205368626
6	9.288571426158813E-4
7	0.003504324674414461
8	0.002153259739700452
9	0.007261974024087799
10	0.0042220779209812785
11	0.01228624675005552
12	0.008275272725123305
13	0.007219753244877987
14	0.015875012982889607
15	0.006037571427003228
16	0.031032272719212398
17	0.038420909080929634
18	0.0221236883059419
19	0.015875012982889607
20	0.015283922073952228
21	0.023010324669347968
22	0.005362038959646223
23	0.016001675320519045
24	0.011779597399537767
25	0.4676373505278864
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2368502.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.386560920837475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.29163841885367	36.42527043258725
2	12.365933170447033	12.461536900714687
3	5.530677001216425	8.360153809657984
4	3.063764550785493	6.174902367410221
5	1.7897019348149685	4.508846278434756
6	1.1612493853995591	3.510681774103198
7	0.7939997264800877	2.8004840912592055
8	0.5679384094026243	2.2893170611719094
9	0.40973162886671477	1.8580470911178026
>10	1.9220680526637872	16.18446776705584
>50	0.07684441617919646	2.62465246907065
>100	0.024985848864697156	2.059934142870171
>500	7.768884841825246E-4	0.26209577426009517
>1k	6.905675414951131E-4	0.4796100402861846
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	4.222077920981278E-5
3	0.0	0.0	0.0	0.0	4.222077920981278E-5
4	0.0	0.0	0.0	0.0	1.2666233762943837E-4
5	0.0	0.0	0.0	0.0	1.2666233762943837E-4
6	0.0	0.0	0.0	0.0	1.2666233762943837E-4
7	0.0	0.0	0.0	1.2666233762943837E-4	1.2666233762943837E-4
8	0.0	0.0	0.0	2.5332467525887673E-4	1.6888311683925113E-4
9	0.0	0.0	0.0	2.5332467525887673E-4	2.5332467525887673E-4
10	0.0	0.0	0.0	2.955454544686895E-4	2.5332467525887673E-4
11	0.0	0.0	0.0	2.955454544686895E-4	2.5332467525887673E-4
12	4.222077920981278E-5	0.0	0.0	3.7998701288831507E-4	2.5332467525887673E-4
13	4.222077920981278E-5	0.0	0.0	4.6442857130794065E-4	3.3776623367850225E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1390	0.0	13.399096	1
GTATAGG	285	0.0	11.002802	1
GTATCAA	2905	0.0	10.271118	1
TCGCGCA	105	4.1209456E-5	9.94924	18
TACGCTC	105	4.1295592E-5	9.947349	4
GTATTAA	280	0.0	9.841792	1
GTATTGA	210	6.730261E-11	9.502419	1
GTCTAGG	280	0.0	9.502419	1
ATTCGCC	90	0.0011152473	9.498806	11
CGTGCGC	120	1.7056931E-5	9.497804	10
CGTACAC	110	6.873514E-5	9.495597	3
GTCTAGA	295	0.0	9.341361	1
GTCCTAC	125	2.7466473E-5	9.122322	1
GTTCGCG	115	1.1119497E-4	9.0862	16
TGTACGC	95	0.0018241742	9.00001	2
GACTTAG	190	1.0670192E-8	8.995639	7
GTATTAG	180	4.202775E-8	8.974507	1
TCGTGCG	85	0.0074629006	8.93741	9
TACCGTA	85	0.007466779	8.936844	7
TAGGGAC	170	1.6986087E-7	8.936656	4
>>END_MODULE