FastQCFastQC Report
Mon 15 Aug 2016
SRR2921149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2368502
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46000.1942155843651388No Hit
TATCAACGCAGAGTACTTTTTTTTT28170.11893593503404262No Hit
GGTATCAACGCAGAGTACTTTTTTT27920.1178804155537973No Hit
GGTATCAACGCAGAGTACATGGGGG24170.1020476233501175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.014.1141481
TAGGTCG500.001501158913.2985795
TACGCTG550.00307171612.0896195
GACCGCG802.8940274E-511.8644587
GACTCGA600.005910602411.07489718
AAACCGC600.00591524811.0737286
GTATCAA33350.010.7856081
ACCGTCC909.543589E-510.5528688
TATACCG1002.4026569E-510.4488845
TAATACC1201.5121605E-610.2981834
TGTACGC750.002634574710.139752
CGAATAA750.002647541510.13375111
ACCGCGG856.6111126E-410.0562638
CAGGACT3950.09.6263444
TTAGACA2500.09.5060154
TACTAGC1106.7915505E-59.5060152
TCGCCTG1401.0700533E-69.49276918
ATTTGCG900.00112175219.49276816
GCACCGT1701.6892955E-89.4917676
GAACCGT1106.903919E-59.4917666
Produced by FastQC (version 0.11.5)