FastQCFastQC Report
Mon 15 Aug 2016
SRR2921148_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921148_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938947
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23870.1231080581367103No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAC400.005292916714.2446413
TGTACGC500.001499366613.30048752
GGTATCA11450.012.5307841
GTATTAG1604.5474735E-1111.283411
GTATCAA23400.010.4763641
TCTAGGA2001.8189894E-1210.4503832
GTTCTAG1659.258656E-1010.3656211
GTCTTAG1659.258656E-1010.3656211
CTAGTCT1201.5313271E-610.2877964
AAGACCG1856.002665E-1110.2664095
CTAGGAC2151.8189894E-1210.1589693
CTTACAC2002.5465852E-119.971253
TAGGACT2650.09.6756064
GTACTAA900.00111182559.501821
GTGTACG1002.750332E-49.501821
CGTGTCA900.00111603159.49789710
TAATACT1804.2091415E-99.49642854
TAGACAG2201.6370905E-119.4964285
ACTAAAC800.0045303339.4964283
CGTAAAC800.0045303339.4964283
Produced by FastQC (version 0.11.5)