##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921148_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1938947
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.893787194802126	30.0	26.0	31.0	2.0	34.0
2	25.520086418040307	31.0	25.0	31.0	2.0	34.0
3	25.312594413359417	30.0	25.0	31.0	2.0	34.0
4	29.07986035719388	35.0	28.0	37.0	2.0	37.0
5	28.654476888744252	35.0	28.0	37.0	2.0	37.0
6	29.12400545244403	35.0	29.0	37.0	2.0	37.0
7	28.912208018063414	35.0	28.0	37.0	2.0	37.0
8	28.640056690564517	35.0	28.0	37.0	2.0	37.0
9	30.216928054248	37.0	29.0	39.0	2.0	39.0
10	29.566861806949856	35.0	27.0	39.0	2.0	39.0
11	30.00952527325399	37.0	29.0	39.0	2.0	39.0
12	29.72888222318609	37.0	27.0	39.0	2.0	39.0
13	29.701677766333994	37.0	27.0	39.0	2.0	39.0
14	30.708659906640047	37.0	29.0	40.0	2.0	41.0
15	30.380587504454738	37.0	27.0	40.0	2.0	41.0
16	30.288365282805565	37.0	27.0	40.0	2.0	41.0
17	30.566181540805395	37.0	28.0	40.0	2.0	41.0
18	30.60358844259281	37.0	29.0	40.0	2.0	41.0
19	30.300086077649365	37.0	27.0	40.0	2.0	41.0
20	30.20609227585901	37.0	27.0	40.0	2.0	41.0
21	30.128316555326165	37.0	27.0	40.0	2.0	41.0
22	29.403904284129478	37.0	25.0	40.0	2.0	41.0
23	29.47077305362137	37.0	26.0	40.0	2.0	41.0
24	29.114018588440015	37.0	25.0	40.0	2.0	41.0
25	28.63444333444906	37.0	22.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	229621.0
3	10.0
4	1836.0
5	9488.0
6	10733.0
7	8991.0
8	7867.0
9	7285.0
10	6692.0
11	6614.0
12	6223.0
13	6130.0
14	6084.0
15	5967.0
16	6443.0
17	6981.0
18	7934.0
19	9871.0
20	12325.0
21	15159.0
22	18190.0
23	20933.0
24	24370.0
25	26872.0
26	30251.0
27	33284.0
28	36970.0
29	43222.0
30	50758.0
31	59933.0
32	73784.0
33	93271.0
34	119398.0
35	152121.0
36	197874.0
37	255225.0
38	330237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.21766601713143	17.476006147626617	12.151777657741878	26.15455017750008
2	16.066662951473383	19.862352088867425	38.28068078852914	25.790304171130057
3	19.958714112768654	23.02415513259816	28.83517986750758	28.181950887125602
4	13.877281962432242	15.278297159439367	33.624467888114786	37.2199529900136
5	14.335937741777752	36.38699707648791	33.149676804397025	16.127388377337315
6	34.40173662166435	33.50119163232225	16.94567693535169	15.151394810661717
7	29.640947721418836	31.081520175028704	19.62141068925288	19.656121414299584
8	27.382076602952075	33.92607278224278	19.677478084302138	19.01437253050301
9	27.305774416296856	15.738116016480058	18.553115593544142	38.402993973678946
10	16.357150925091638	28.11981500746046	31.836995310200845	23.68603875724706
11	36.22382715425332	22.30472686984267	21.247909731341803	20.223536244562208
12	23.88268170831971	24.305367150919594	28.59473466495151	23.217216475809188
13	28.95055271529901	21.159361086697285	24.60666229280913	25.283423905194574
14	23.109185613582977	20.503832024354942	25.176075045572667	31.21090731648941
15	24.77641177781262	27.59326233526164	22.810279591011493	24.820046295914242
16	24.750538337802716	26.721778150140274	24.232578439454926	24.295105072602087
17	23.830753466851444	26.778725156252097	25.1875695872592	24.202951789637265
18	23.853287677027375	26.545929879259774	25.83028894911743	23.770493494595424
19	24.760774373520555	25.972132168193244	26.106452373325574	23.160641084960627
20	25.271498884293557	25.220072193376737	25.667283236755694	23.841145685574013
21	25.74669904855839	24.962291053229972	25.275827275484748	24.01518262272689
22	24.74546583620275	24.400052402575568	26.682040151926206	24.172441609295475
23	25.085703969256954	24.705387769839838	25.654243932633534	24.55466432826967
24	24.65628077096268	25.20421989984108	25.659520539077608	24.479978790118633
25	24.27999019658134	24.94120280485549	26.260052778870214	24.518754219692955
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1173.0
1	1173.0
2	1188.0
3	1203.0
4	1203.0
5	1203.0
6	1576.0
7	1949.0
8	1949.0
9	1949.0
10	2494.0
11	3039.0
12	3039.0
13	3039.0
14	4260.5
15	5482.0
16	5482.0
17	5482.0
18	9185.0
19	12888.0
20	12888.0
21	12888.0
22	19760.0
23	26632.0
24	26632.0
25	26632.0
26	38725.5
27	50819.0
28	50819.0
29	50819.0
30	67081.5
31	83344.0
32	83344.0
33	83344.0
34	102969.5
35	122595.0
36	122595.0
37	122595.0
38	146122.0
39	169649.0
40	169649.0
41	169649.0
42	191814.0
43	213979.0
44	213979.0
45	213979.0
46	234883.0
47	255787.0
48	255787.0
49	255787.0
50	264633.0
51	273479.0
52	273479.0
53	273479.0
54	261146.5
55	248814.0
56	248814.0
57	248814.0
58	221357.0
59	193900.0
60	193900.0
61	193900.0
62	163290.0
63	132680.0
64	132680.0
65	132680.0
66	105339.5
67	77999.0
68	77999.0
69	77999.0
70	57842.0
71	37685.0
72	37685.0
73	37685.0
74	26897.0
75	16109.0
76	16109.0
77	16109.0
78	11212.5
79	6316.0
80	6316.0
81	6316.0
82	4347.0
83	2378.0
84	2378.0
85	2378.0
86	1590.5
87	803.0
88	803.0
89	803.0
90	499.0
91	195.0
92	195.0
93	195.0
94	115.5
95	36.0
96	36.0
97	36.0
98	25.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03290445793515759
2	0.04945983567369299
3	0.013925084079141926
4	0.01083062095044372
5	0.009438112542529527
6	5.157438547830343E-4
7	0.003352335056089723
8	0.0020629754191321373
9	0.006137351871918107
10	0.0029913143577415986
11	0.010366451481138989
12	0.007890880978180425
13	0.007994029749137032
14	0.014698699861316477
15	0.005931054330004895
16	0.029552122879067867
17	0.037804024555596415
18	0.02088762611871289
19	0.015266018101577815
20	0.013667212151750407
21	0.021403369973495925
22	0.005724756788091681
23	0.015317592487056119
24	0.011758959889053181
25	0.46458206438855726
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1938947.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.002775065064554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.24563091423443	40.6347288089027
2	10.983694232091842	11.862999379982057
3	5.012230786518728	8.120231152156874
4	2.708337998927278	5.850310710249474
5	1.6424686834506337	4.434893343189864
6	1.0779416823106502	3.4927105321847582
7	0.7272085825907544	2.748989705772305
8	0.5149915262328787	2.224877724125476
9	0.3677945468413969	1.7875733564909977
>10	1.648038859845494	14.754627046027649
>50	0.05486447413408648	1.9900984138127114
>100	0.01532478965655215	1.3453340061398846
>500	9.819487768374094E-4	0.36302940677293255
>1k	4.909743884187046E-4	0.3895964141923363
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2387	0.1231080581367103	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	5.157438547830343E-5	0.0
12	0.0	0.0	0.0	5.157438547830343E-5	0.0
13	0.0	0.0	0.0	5.157438547830343E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTAC	40	0.0052929167	14.244641	3
TGTACGC	50	0.0014993666	13.3004875	2
GGTATCA	1145	0.0	12.530784	1
GTATTAG	160	4.5474735E-11	11.28341	1
GTATCAA	2340	0.0	10.476364	1
TCTAGGA	200	1.8189894E-12	10.450383	2
GTTCTAG	165	9.258656E-10	10.365621	1
GTCTTAG	165	9.258656E-10	10.365621	1
CTAGTCT	120	1.5313271E-6	10.287796	4
AAGACCG	185	6.002665E-11	10.266409	5
CTAGGAC	215	1.8189894E-12	10.158969	3
CTTACAC	200	2.5465852E-11	9.97125	3
TAGGACT	265	0.0	9.675606	4
GTACTAA	90	0.0011118255	9.50182	1
GTGTACG	100	2.750332E-4	9.50182	1
CGTGTCA	90	0.0011160315	9.497897	10
TAATACT	180	4.2091415E-9	9.4964285	4
TAGACAG	220	1.6370905E-11	9.496428	5
ACTAAAC	80	0.004530333	9.496428	3
CGTAAAC	80	0.004530333	9.496428	3
>>END_MODULE