FastQCFastQC Report
Mon 15 Aug 2016
SRR2921148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938947
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46420.23940829739028452No Hit
GGTATCAACGCAGAGTACTTTTTTT30920.1594679998989142No Hit
TATCAACGCAGAGTACTTTTTTTTT30590.1577660451781302No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21180.10923454844304667No Hit
GGTATCAACGCAGAGTACATGGGGG19530.1007247748391266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.013.7763591
TATACCG658.024531E-411.6923875
GATTATA907.3412357E-611.6282961
CCGTATC600.00587624711.0834099
TCCGTAT600.005885283511.08112058
TCGATAA600.005905659411.07597718
GTATCAA27200.010.6333621
TATACAC4300.010.6107483
GTTCGCG1106.0965194E-610.3564897
CTTATAC4000.010.2276151
CCCTAGT750.002615782210.1483311
CAGCGTA750.00264792610.1334039
TAGGCTG1901.0004442E-1010.0000685
ACACCGT1054.1368658E-59.94551856
GTAGTAA1252.5220888E-69.8946231
CCCTACG1002.7149936E-49.514061
GTCTAGG2002.564775E-109.514061
TAAAGGT1106.794167E-59.5054624
GTAATAC800.00449493579.5054623
CGTAGGA800.00449493579.5054622
Produced by FastQC (version 0.11.5)