##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921148_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1938947
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.004205891135754	31.0	30.0	33.0	25.0	34.0
2	29.314692975104528	31.0	30.0	34.0	25.0	34.0
3	29.44768681144972	31.0	30.0	34.0	25.0	34.0
4	33.099435415202166	35.0	33.0	37.0	28.0	37.0
5	32.57995396470352	35.0	33.0	37.0	25.0	37.0
6	32.50654607887683	35.0	33.0	37.0	25.0	37.0
7	32.347570098615385	35.0	33.0	37.0	22.0	37.0
8	32.26606142406162	35.0	33.0	37.0	22.0	37.0
9	33.601360944884	38.0	34.0	39.0	19.0	39.0
10	33.45269416853581	38.0	33.0	39.0	18.0	39.0
11	33.47527859193676	38.0	34.0	39.0	18.0	39.0
12	33.3420459661868	38.0	33.0	39.0	17.0	39.0
13	33.30266943861797	38.0	33.0	39.0	17.0	39.0
14	34.216866164985426	39.0	33.0	41.0	17.0	41.0
15	34.18036800387014	39.0	33.0	41.0	17.0	41.0
16	34.14919077210465	39.0	33.0	41.0	17.0	41.0
17	34.121456646313696	39.0	33.0	41.0	17.0	41.0
18	34.07009526304741	39.0	33.0	41.0	16.0	41.0
19	34.10207499225095	39.0	34.0	41.0	16.0	41.0
20	34.03648578326277	39.0	34.0	41.0	11.0	41.0
21	33.960045839313814	39.0	34.0	41.0	10.0	41.0
22	33.869643161984314	39.0	34.0	41.0	9.0	41.0
23	33.7356549714871	39.0	34.0	41.0	2.0	41.0
24	33.647508673522275	39.0	34.0	41.0	2.0	41.0
25	33.506476453456436	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83719.0
3	2.0
4	1425.0
5	11435.0
6	12574.0
7	9270.0
8	7248.0
9	6130.0
10	5505.0
11	5055.0
12	4539.0
13	4351.0
14	4160.0
15	4112.0
16	4089.0
17	4336.0
18	4741.0
19	5458.0
20	7157.0
21	8883.0
22	11098.0
23	13444.0
24	15764.0
25	18047.0
26	20282.0
27	22376.0
28	25707.0
29	30736.0
30	38824.0
31	48763.0
32	61982.0
33	79861.0
34	103587.0
35	135922.0
36	183805.0
37	276725.0
38	657835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.0528440321504	18.467946398103066	9.328809548270256	32.15040002147628
2	14.874981110881318	20.403806808540924	35.028497426696035	29.69271465388172
3	18.372601303520497	23.178507596990098	27.388996437814477	31.05989466167493
4	13.441931651999633	15.977709961164265	32.19614367260332	38.38421471423278
5	14.97045823794021	37.22262019533484	32.0488378382284	15.75808372849655
6	34.83802552475109	34.27562274863526	15.891849904890801	14.994501821722858
7	30.38081882468471	31.43368330822461	18.887761675299565	19.297736191791124
8	28.001374019579778	34.82711543053903	18.057371506733162	19.114139043148032
9	27.321488711094055	15.613745455633211	18.27386008533533	38.7909057479374
10	17.06427980164471	28.6224725301646	30.358674433777132	23.95457323441356
11	36.437902850207934	22.263506909540286	20.985372188848906	20.313218051402874
12	24.07478083453588	24.402123627991703	28.172006853207876	23.351088684264543
13	29.195046599307844	20.729342348893944	24.667925131648847	25.407685920149365
14	23.12975791403141	20.168859504268706	25.009649999174332	31.691732582525557
15	24.88247435018491	27.644127225352626	22.2787775991185	25.19462082534396
16	25.27406215427939	26.61323389473847	23.532906799526533	24.579797151455605
17	23.768079615291782	26.835038402742967	25.12455361839866	24.272328363566597
18	23.92115681780202	26.339618203912035	25.977029516679078	23.76219546160686
19	24.87202353168117	25.764003397920295	25.84276211514865	23.521210955249884
20	25.2293284618979	25.098327833713785	25.55466388023861	24.117679824149707
21	25.565710053158952	24.738976455526704	25.54239562926765	24.152917862046696
22	25.26241521017973	24.572028023963956	25.425702662111533	24.73985410374478
23	25.00840698470857	24.52771313511417	25.5207688625917	24.943111017585558
24	24.629038489013638	25.110982853942076	25.54728073310835	24.712697923935934
25	24.56984267129703	24.886845568029827	25.69232017085919	24.850991589813955
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	834.0
1	834.0
2	892.0
3	950.0
4	950.0
5	950.0
6	1187.0
7	1424.0
8	1424.0
9	1424.0
10	1847.0
11	2270.0
12	2270.0
13	2270.0
14	3032.0
15	3794.0
16	3794.0
17	3794.0
18	7264.5
19	10735.0
20	10735.0
21	10735.0
22	17560.5
23	24386.0
24	24386.0
25	24386.0
26	36082.5
27	47779.0
28	47779.0
29	47779.0
30	62427.0
31	77075.0
32	77075.0
33	77075.0
34	95667.0
35	114259.0
36	114259.0
37	114259.0
38	137074.5
39	159890.0
40	159890.0
41	159890.0
42	182985.5
43	206081.0
44	206081.0
45	206081.0
46	228861.0
47	251641.0
48	251641.0
49	251641.0
50	266790.0
51	281939.0
52	281939.0
53	281939.0
54	270551.0
55	259163.0
56	259163.0
57	259163.0
58	231436.5
59	203710.0
60	203710.0
61	203710.0
62	173252.5
63	142795.0
64	142795.0
65	142795.0
66	113308.0
67	83821.0
68	83821.0
69	83821.0
70	61961.5
71	40102.0
72	40102.0
73	40102.0
74	28386.0
75	16670.0
76	16670.0
77	16670.0
78	11541.5
79	6413.0
80	6413.0
81	6413.0
82	4325.0
83	2237.0
84	2237.0
85	2237.0
86	1492.0
87	747.0
88	747.0
89	747.0
90	476.5
91	206.0
92	206.0
93	206.0
94	114.0
95	22.0
96	22.0
97	22.0
98	13.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0993322664312124
2	0.0
3	0.04182682662290409
4	0.053688935282913866
5	0.06967699478118793
6	0.0063436494138313215
7	0.0023724217320019577
8	0.005673182402613377
9	4.125950838264274E-4
10	1.0314877095660685E-4
11	0.005466884860700164
12	5.157438547830342E-5
13	0.003971227681829364
14	0.057918034892134755
15	0.022383283297583687
16	0.003197611899654813
17	0.0011862108660009789
18	0.005724756788091681
19	0.005879479944526591
20	0.0012893596369575858
21	0.0120168318164447
22	0.00180510349174062
23	0.004280673994699185
24	0.00691096765409266
25	0.17112381101701077
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1938947.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.75570415168902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.09293772452137	24.452112285124898
2	14.041397395718713	10.883684866895422
3	7.296023483831683	8.482875828694683
4	4.210846204436899	6.5277723890967625
5	2.776335104489341	5.379941096776878
6	1.911270270256684	4.4443575088792056
7	1.3076132327010526	3.5474230113976977
8	0.9752365440020054	3.02367831818059
9	0.7217070601552874	2.5173238776807194
>10	3.479205862211838	22.758297890900288
>50	0.14017665383297262	3.65227528040359
>100	0.04456640578384585	2.8509528197891614
>500	0.0013420290290997675	0.36293471194305843
>1k	0.0013420290290997675	1.1163701142370028
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4642	0.23940829739028452	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3092	0.1594679998989142	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3059	0.1577660451781302	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2118	0.10923454844304667	No Hit
GGTATCAACGCAGAGTACATGGGGG	1953	0.1007247748391266	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1250	0.0	13.776359	1
TATACCG	65	8.024531E-4	11.692387	5
GATTATA	90	7.3412357E-6	11.628296	1
CCGTATC	60	0.005876247	11.083409	9
TCCGTAT	60	0.0058852835	11.0811205	8
TCGATAA	60	0.0059056594	11.075977	18
GTATCAA	2720	0.0	10.633362	1
TATACAC	430	0.0	10.610748	3
GTTCGCG	110	6.0965194E-6	10.356489	7
CTTATAC	400	0.0	10.227615	1
CCCTAGT	75	0.0026157822	10.148331	1
CAGCGTA	75	0.002647926	10.133403	9
TAGGCTG	190	1.0004442E-10	10.000068	5
ACACCGT	105	4.1368658E-5	9.9455185	6
GTAGTAA	125	2.5220888E-6	9.894623	1
CCCTACG	100	2.7149936E-4	9.51406	1
GTCTAGG	200	2.564775E-10	9.51406	1
TAAAGGT	110	6.794167E-5	9.505462	4
GTAATAC	80	0.0044949357	9.505462	3
CGTAGGA	80	0.0044949357	9.505462	2
>>END_MODULE