FastQCFastQC Report
Mon 15 Aug 2016
SRR2921147_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921147_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658731
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.013.3345751
CTAAGAC1551.8189894E-1212.2531133
CGGAAAT1103.8295184E-812.088310
CGCTCGA600.005886970611.08094110
AACGACC600.005891918711.079697
GTATCAA27100.011.0095621
GTACTAG951.3581053E-511.002181
TTAGGAC1502.1500455E-910.7623183
AGACTAG803.7847075E-410.6837866
TATACCG1002.4113575E-510.4455825
TTACCCT1752.382876E-1010.3097324
CTTAGAC2950.010.3009223
ATTATTC2151.8189894E-1210.1586863
TATCGGA750.00264841210.1334332
TTTATAC1356.4829146E-79.8478723
TTAGACC1356.4862616E-79.8475024
GAATAAT1451.6136437E-79.8295331
TAAGACT2850.09.6623984
GTATTAT2201.6370905E-119.5018831
GTATAGG2201.6370905E-119.5018831
Produced by FastQC (version 0.11.5)