##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921147_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2658731
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.249583353863176	30.0	26.0	31.0	2.0	34.0
2	26.12048567530901	31.0	26.0	31.0	2.0	34.0
3	25.877061650840194	31.0	25.0	31.0	2.0	34.0
4	29.794484285924376	35.0	30.0	37.0	2.0	37.0
5	29.231785013226233	35.0	28.0	37.0	2.0	37.0
6	29.736490453528393	35.0	30.0	37.0	2.0	37.0
7	29.616343661694245	35.0	30.0	37.0	2.0	37.0
8	29.391142240414695	35.0	29.0	37.0	2.0	37.0
9	30.952085412175958	37.0	30.0	39.0	2.0	39.0
10	30.30118240619303	37.0	29.0	39.0	2.0	39.0
11	30.715391290055294	37.0	30.0	39.0	2.0	39.0
12	30.432696275027446	37.0	30.0	39.0	2.0	39.0
13	30.422792302041838	37.0	30.0	39.0	2.0	39.0
14	31.476266309002302	38.0	30.0	40.0	2.0	41.0
15	31.12083772295881	38.0	29.0	40.0	2.0	41.0
16	31.036057803516037	37.0	29.0	40.0	2.0	41.0
17	31.321365719209652	38.0	30.0	40.0	2.0	41.0
18	31.36148711546975	38.0	30.0	40.0	2.0	41.0
19	31.06302668453484	38.0	30.0	40.0	2.0	41.0
20	30.9292485776109	38.0	29.0	40.0	2.0	41.0
21	30.869128919021893	38.0	30.0	40.0	2.0	41.0
22	30.133417032411327	37.0	27.0	40.0	2.0	41.0
23	30.208841736903807	37.0	27.0	40.0	2.0	41.0
24	29.834820822414905	37.0	27.0	40.0	2.0	41.0
25	29.352429034753797	37.0	26.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	266637.0
3	4.0
4	2252.0
5	12059.0
6	13231.0
7	11631.0
8	10027.0
9	9313.0
10	8927.0
11	8358.0
12	7986.0
13	8005.0
14	7895.0
15	7994.0
16	8595.0
17	9103.0
18	10353.0
19	12731.0
20	16221.0
21	20363.0
22	24842.0
23	29032.0
24	33524.0
25	37555.0
26	41697.0
27	46707.0
28	52133.0
29	60820.0
30	71022.0
31	84431.0
32	105262.0
33	131725.0
34	167741.0
35	213944.0
36	279690.0
37	358709.0
38	468212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.300949336608156	16.87032706060825	12.090233631919864	25.738489970863732
2	16.395240276046227	18.4705919098541	38.210102352654744	26.924065461444936
3	20.196333123804	21.987978207444574	28.4058579436257	29.40983072512573
4	14.184076763036742	14.284773894127706	33.31363526423311	38.21751407860244
5	14.892058782373713	35.704070440145586	33.05662174382091	16.347249033659786
6	35.9857404906201	32.27131733087801	16.476631397775694	15.266310780726192
7	31.34468649784364	29.97673248222213	19.32821342625295	19.350367593681288
8	27.884507248857055	33.4326803187278	19.255913145358907	19.426899287056244
9	27.69420056700017	15.276392834748828	17.87239148514248	39.15701511310852
10	16.78363131799064	26.721200855475622	31.749452163100056	24.745715663433682
11	37.293060728266745	21.671884621446566	20.89748571441468	20.137568935872007
12	24.737033347978116	23.156600383974194	28.02661789753065	24.07974837051704
13	29.58482617240763	19.904571143773335	24.54770972625798	25.962892957561053
14	23.761127777830023	19.231207621340506	24.46367543471923	32.54398916611024
15	25.526741722307673	26.486584283753505	22.181849504299507	25.804824489639323
16	25.506796858745506	25.879494446595253	23.472573844424904	25.141134850234337
17	24.798509989840838	25.94096399142115	24.408699251232267	24.851826767505738
18	24.848755944518704	25.748301149571446	24.92301892953707	24.479923976372785
19	25.782770306998913	24.95261038798352	25.219624848069223	24.044994456948338
20	26.0682751107473	24.41797476887519	25.03460853235747	24.47914158802003
21	26.380388177920334	24.17382118353974	24.613230974929188	24.832559663610738
22	25.522355329888143	23.587001045293675	25.78866260711063	25.10198101770755
23	25.665021128172565	23.958892509834296	25.119822864169905	25.256263497823234
24	25.324753244711207	24.464988942609526	24.865041154407365	25.3452166582719
25	25.147556494677385	24.066861040702165	25.408812731348178	25.37676973327227
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	523.0
1	523.0
2	570.5
3	618.0
4	618.0
5	618.0
6	818.5
7	1019.0
8	1019.0
9	1019.0
10	1219.5
11	1420.0
12	1420.0
13	1420.0
14	2006.0
15	2592.0
16	2592.0
17	2592.0
18	4637.0
19	6682.0
20	6682.0
21	6682.0
22	12679.0
23	18676.0
24	18676.0
25	18676.0
26	31964.0
27	45252.0
28	45252.0
29	45252.0
30	66628.5
31	88005.0
32	88005.0
33	88005.0
34	117790.0
35	147575.0
36	147575.0
37	147575.0
38	185677.5
39	223780.0
40	223780.0
41	223780.0
42	260260.5
43	296741.0
44	296741.0
45	296741.0
46	328999.0
47	361257.0
48	361257.0
49	361257.0
50	376346.0
51	391435.0
52	391435.0
53	391435.0
54	376223.5
55	361012.0
56	361012.0
57	361012.0
58	324681.5
59	288351.0
60	288351.0
61	288351.0
62	245962.0
63	203573.0
64	203573.0
65	203573.0
66	161232.5
67	118892.0
68	118892.0
69	118892.0
70	89278.5
71	59665.0
72	59665.0
73	59665.0
74	42679.0
75	25693.0
76	25693.0
77	25693.0
78	18185.5
79	10678.0
80	10678.0
81	10678.0
82	7165.5
83	3653.0
84	3653.0
85	3653.0
86	2430.0
87	1207.0
88	1207.0
89	1207.0
90	780.5
91	354.0
92	354.0
93	354.0
94	204.5
95	55.0
96	55.0
97	55.0
98	39.0
99	23.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03291043734774221
2	0.05077610333651656
3	0.014743875931788511
4	0.009929549096918793
5	0.009290146314162659
6	5.265669975638754E-4
7	0.003347461627370351
8	0.0013540294223071082
9	0.006694923254740702
10	0.003497909340960029
11	0.010606563808072346
12	0.008237012319034908
13	0.007748057249868452
14	0.015270442929352385
15	0.006394027827561343
16	0.03054088585870477
17	0.03877789817773968
18	0.022943276322426
19	0.016850143922044012
20	0.0160602934256982
21	0.023319395606400194
22	0.005566565402818111
23	0.0162483530676853
24	0.012336712514353653
25	0.4621377642190955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2658731.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.39663191499384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.28601792594677	47.886084406790104
2	10.888210842148336	13.152225248921562
3	3.954872551005315	7.165829452013421
4	1.8609160664220206	4.49572250753556
5	1.0555114514351722	3.1874668307195493
6	0.6624240685655078	2.4004909584470173
7	0.4271583382571715	1.805924744759717
8	0.3191723072212831	1.542154588536253
9	0.26294787199678293	1.4293049254038552
>10	1.2159024537358243	12.66128657880473
>50	0.049438878216596795	2.0212696268075523
>100	0.016405759977661513	1.6827635721413998
>500	7.66113803729264E-4	0.33631082325682327
>1k	2.5537126790222515E-4	0.23316573586246542
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.7611928397419675E-5	0.0
4	0.0	0.0	0.0	3.7611928397419675E-5	0.0
5	0.0	0.0	0.0	3.7611928397419675E-5	3.7611928397419675E-5
6	0.0	0.0	0.0	3.7611928397419675E-5	1.504477135896787E-4
7	0.0	0.0	0.0	3.7611928397419675E-5	1.504477135896787E-4
8	0.0	0.0	0.0	3.7611928397419675E-5	1.504477135896787E-4
9	0.0	0.0	0.0	3.7611928397419675E-5	1.504477135896787E-4
10	0.0	0.0	0.0	3.7611928397419675E-5	1.504477135896787E-4
11	0.0	0.0	0.0	3.7611928397419675E-5	1.504477135896787E-4
12	0.0	0.0	0.0	3.7611928397419675E-5	3.008954271793574E-4
13	0.0	0.0	0.0	3.7611928397419675E-5	3.3850735557677705E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1190	0.0	13.334575	1
CTAAGAC	155	1.8189894E-12	12.253113	3
CGGAAAT	110	3.8295184E-8	12.0883	10
CGCTCGA	60	0.0058869706	11.080941	10
AACGACC	60	0.0058919187	11.07969	7
GTATCAA	2710	0.0	11.009562	1
GTACTAG	95	1.3581053E-5	11.00218	1
TTAGGAC	150	2.1500455E-9	10.762318	3
AGACTAG	80	3.7847075E-4	10.683786	6
TATACCG	100	2.4113575E-5	10.445582	5
TTACCCT	175	2.382876E-10	10.309732	4
CTTAGAC	295	0.0	10.300922	3
ATTATTC	215	1.8189894E-12	10.158686	3
TATCGGA	75	0.002648412	10.133433	2
TTTATAC	135	6.4829146E-7	9.847872	3
TTAGACC	135	6.4862616E-7	9.847502	4
GAATAAT	145	1.6136437E-7	9.829533	1
TAAGACT	285	0.0	9.662398	4
GTATTAT	220	1.6370905E-11	9.501883	1
GTATAGG	220	1.6370905E-11	9.501883	1
>>END_MODULE