FastQCFastQC Report
Mon 15 Aug 2016
SRR2921147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658731
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACATGGGGG32360.12171220029405007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCG400.00527926314.25046611
GGTATCA13350.013.7552481
CTAGACA2200.011.2327154
CTAGCAC2150.011.0522823
CTACTAG951.3380573E-511.0169511
TATACCG700.001493942810.8554545
CCCTTAT1502.0809239E-910.7832581
GTATCAA28800.010.6709321
TAGCACA3500.010.5908464
GCGTGTA1355.689799E-810.5559019
ATAGCAC2900.010.48823453
CGTGTAA1201.5241167E-610.29200310
CTTAAAC1951.4551915E-1110.2361143
GTTTAAG2250.010.148951
CCCTATA1502.4272595E-810.1386272
ACGACCG750.002652048610.1317575
TACGACA856.566904E-410.0636994
GCGAATC1054.1269916E-59.94802515
TAACGAG1151.0289174E-59.9115025
TATTAAG1554.0339728E-89.8115752
Produced by FastQC (version 0.11.5)