##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2658731
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.585738083318695	31.0	30.0	33.0	26.0	34.0
2	29.88886088889775	31.0	30.0	34.0	26.0	34.0
3	30.022920709165387	31.0	30.0	34.0	26.0	34.0
4	33.71083310045281	35.0	35.0	37.0	30.0	37.0
5	33.281829188436134	35.0	35.0	37.0	27.0	37.0
6	33.235598110527164	36.0	35.0	37.0	27.0	37.0
7	33.102838534624226	36.0	35.0	37.0	26.0	37.0
8	33.039418429318346	37.0	35.0	37.0	26.0	37.0
9	34.4663386405018	39.0	35.0	39.0	27.0	39.0
10	34.327669102289775	38.0	35.0	39.0	26.0	39.0
11	34.352742718236634	38.0	35.0	39.0	27.0	39.0
12	34.218028074295596	38.0	35.0	39.0	26.0	39.0
13	34.19422574152857	38.0	35.0	39.0	26.0	39.0
14	35.169164537518085	39.0	35.0	41.0	25.0	41.0
15	35.14305772189815	39.0	35.0	41.0	25.0	41.0
16	35.11052791726579	39.0	35.0	41.0	25.0	41.0
17	35.077933796235875	39.0	35.0	41.0	25.0	41.0
18	35.02605039772734	39.0	35.0	41.0	25.0	41.0
19	35.05450946334924	39.0	35.0	41.0	25.0	41.0
20	34.99139815197551	39.0	35.0	41.0	25.0	41.0
21	34.926691718718445	39.0	35.0	41.0	24.0	41.0
22	34.84825204204562	39.0	35.0	41.0	24.0	41.0
23	34.71750395207338	39.0	35.0	41.0	22.0	41.0
24	34.63523613332827	39.0	35.0	41.0	22.0	41.0
25	34.49842537661764	39.0	34.0	41.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83210.0
3	6.0
4	1300.0
5	11176.0
6	12591.0
7	9355.0
8	7525.0
9	6572.0
10	5879.0
11	5264.0
12	5024.0
13	4716.0
14	4665.0
15	4580.0
16	4718.0
17	4919.0
18	5468.0
19	6484.0
20	8025.0
21	10439.0
22	13116.0
23	16157.0
24	19290.0
25	22302.0
26	25500.0
27	28497.0
28	33029.0
29	40428.0
30	50983.0
31	65545.0
32	83740.0
33	108662.0
34	143351.0
35	190642.0
36	259995.0
37	396481.0
38	959097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04762094805155	17.947716842800006	9.192793624486406	31.811868584662033
2	15.478850624602488	19.916794891999228	34.52620817976697	30.07814630363132
3	18.94859743632484	22.484176923461877	26.910762777456487	31.6564628627568
4	14.040951033371968	15.140668041485405	31.75549049418211	39.062890430960515
5	15.612956262282518	36.758367357078626	31.665363683003463	15.963312697635395
6	36.1977888294925	33.22631451645363	15.533489742556739	15.04240691149713
7	31.92570217001028	30.436187374724998	18.10734090333782	19.5307695519269
8	29.142684753051334	34.18115299009527	17.362560642063897	19.313601614789498
9	27.876530245335733	15.429431561907139	17.50087636023897	39.19316183251815
10	17.42517474521651	27.52176980872056	29.977718668476054	25.075336777586877
11	37.84822911396453	21.627473513352882	20.51474280727426	20.009554565408337
12	25.111086872303694	23.29962049549973	27.3611461111132	24.228146521083374
13	29.796960978396896	19.789825517866298	24.423436358048907	25.9897771456879
14	23.92154117485	19.115494022173998	24.315935424575063	32.64702937840094
15	25.789960570158293	26.674489182093186	21.583935226863606	25.951615020884912
16	26.047732447328915	25.936580670423886	22.693055214410084	25.322631667837115
17	24.99134922067762	26.040162183306254	24.193032737557925	24.775455858458205
18	25.00233222640351	25.67337394918154	24.90426586779135	24.4200279566236
19	26.034565902489337	24.880588672079828	24.808554432682406	24.27629099274843
20	26.203366447161553	24.433521845949237	24.724490241124634	24.638621465764572
21	26.319452490982442	24.11129854319624	24.650922371515808	24.91832659430551
22	26.23446690422834	23.74909635540671	24.3370201280486	25.67941661231635
23	25.79470596001729	23.898222267527654	24.750846248202464	25.556225524252596
24	25.48488614767924	24.48411725141403	24.563828170493345	25.467168430413384
25	25.56385622528248	24.13737618865486	24.58809941459867	25.71066817146399
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	300.0
1	300.0
2	286.5
3	273.0
4	273.0
5	273.0
6	433.5
7	594.0
8	594.0
9	594.0
10	771.5
11	949.0
12	949.0
13	949.0
14	1323.0
15	1697.0
16	1697.0
17	1697.0
18	3494.5
19	5292.0
20	5292.0
21	5292.0
22	10982.5
23	16673.0
24	16673.0
25	16673.0
26	29517.5
27	42362.0
28	42362.0
29	42362.0
30	61773.5
31	81185.0
32	81185.0
33	81185.0
34	108363.0
35	135541.0
36	135541.0
37	135541.0
38	172092.5
39	208644.0
40	208644.0
41	208644.0
42	244413.0
43	280182.0
44	280182.0
45	280182.0
46	317161.5
47	354141.0
48	354141.0
49	354141.0
50	374649.0
51	395157.0
52	395157.0
53	395157.0
54	385434.5
55	375712.0
56	375712.0
57	375712.0
58	338822.0
59	301932.0
60	301932.0
61	301932.0
62	260573.5
63	219215.0
64	219215.0
65	219215.0
66	173684.5
67	128154.0
68	128154.0
69	128154.0
70	96635.0
71	65116.0
72	65116.0
73	65116.0
74	46377.5
75	27639.0
76	27639.0
77	27639.0
78	19740.0
79	11841.0
80	11841.0
81	11841.0
82	8085.0
83	4329.0
84	4329.0
85	4329.0
86	2845.0
87	1361.0
88	1361.0
89	1361.0
90	864.5
91	368.0
92	368.0
93	368.0
94	218.5
95	69.0
96	69.0
97	69.0
98	37.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10132653510264861
2	0.0
3	0.039943867958059695
4	0.05307043096875916
5	0.07552475222201871
6	0.012035817087174295
7	0.0030089542717935736
8	0.01143402623281558
9	2.632834987819377E-4
10	1.1283578519225902E-4
11	0.011885369373584616
12	3.7611928397419675E-5
13	0.006694923254740702
14	0.06563281505349733
15	0.022266261611272445
16	0.007672833393073613
17	4.137312123716164E-4
18	0.012299100585956232
19	0.01026805645249557
20	8.650743531406524E-4
21	0.021927754255695667
22	0.0019934322050632423
23	0.008725967388201365
24	0.014104473149032378
25	0.1879091942735087
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2658731.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.72277619191122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.42267577154095	33.60765594680778
2	14.430134741691965	13.772921813937767
3	5.663218291858019	8.10793497204834
4	2.857653629381005	5.4550065835581005
5	1.6458577873744762	3.927245141529317
6	1.0622268748618218	3.0415449248438406
7	0.7270781334530305	2.428873092576807
8	0.5440567912413226	2.07711203872792
9	0.3862592251613916	1.6590026298994323
>10	2.10707723001263	17.90077554966201
>50	0.11183702192268428	3.615579418576256
>100	0.03962395040080434	3.2031017555830577
>500	0.0012692695723613124	0.39050699601362127
>1k	0.0010312815275206133	0.8127391362357923
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACATGGGGG	3236	0.12171220029405007	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8805964198709835E-4
2	0.0	0.0	0.0	0.0	1.8805964198709835E-4
3	0.0	0.0	0.0	0.0	1.8805964198709835E-4
4	0.0	0.0	0.0	0.0	2.2567157038451803E-4
5	0.0	0.0	0.0	0.0	2.2567157038451803E-4
6	0.0	0.0	0.0	0.0	2.2567157038451803E-4
7	0.0	0.0	0.0	0.0	2.2567157038451803E-4
8	0.0	0.0	0.0	0.0	2.2567157038451803E-4
9	0.0	0.0	0.0	0.0	3.008954271793574E-4
10	0.0	0.0	0.0	0.0	3.008954271793574E-4
11	0.0	0.0	0.0	0.0	3.008954271793574E-4
12	0.0	0.0	0.0	0.0	6.394027827561344E-4
13	0.0	0.0	0.0	0.0	7.522385679483934E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCCG	40	0.005279263	14.250466	11
GGTATCA	1335	0.0	13.755248	1
CTAGACA	220	0.0	11.232715	4
CTAGCAC	215	0.0	11.052282	3
CTACTAG	95	1.3380573E-5	11.016951	1
TATACCG	70	0.0014939428	10.855454	5
CCCTTAT	150	2.0809239E-9	10.783258	1
GTATCAA	2880	0.0	10.670932	1
TAGCACA	350	0.0	10.590846	4
GCGTGTA	135	5.689799E-8	10.555901	9
ATAGCAC	290	0.0	10.4882345	3
CGTGTAA	120	1.5241167E-6	10.292003	10
CTTAAAC	195	1.4551915E-11	10.236114	3
GTTTAAG	225	0.0	10.14895	1
CCCTATA	150	2.4272595E-8	10.138627	2
ACGACCG	75	0.0026520486	10.131757	5
TACGACA	85	6.566904E-4	10.063699	4
GCGAATC	105	4.1269916E-5	9.948025	15
TAACGAG	115	1.0289174E-5	9.911502	5
TATTAAG	155	4.0339728E-8	9.811575	2
>>END_MODULE