FastQCFastQC Report
Mon 15 Aug 2016
SRR2921146_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921146_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748604
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30590.17493955177959103No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28520.16310153699751345No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17580.10053734293184735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG46650.024.8709164
GTATAGG4750.022.5598471
TACATGG115250.021.6853712
GTACATG118900.021.5299911
GTATCAA86900.020.6207281
ACATGGG118800.020.5865653
GTATAAT10750.020.4904061
CGTTAGA1550.00567116919.2041721
AACGCAG94350.018.7929786
GTATTAG5450.018.5699061
TCAACGC95900.018.426664
GTACAAG13000.018.3178251
TATCAAC96500.018.2525232
TCTATAC4901.6370905E-1118.2160263
CAACGCA98000.018.0328355
CTATTCG1650.00816066118.0304819
ATCAACG98100.017.9548283
TCGCACT2000.001174813617.8496654
ATGGGGA22350.017.8373765
GAGTACT23650.017.48787312-13
Produced by FastQC (version 0.11.5)