##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921146_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1748604
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.274660243256907	31.0	30.0	34.0	23.0	34.0
2	29.25984556823615	31.0	30.0	34.0	23.0	34.0
3	29.37910641860593	31.0	30.0	34.0	23.0	34.0
4	32.780840601988785	35.0	33.0	37.0	25.0	37.0
5	32.89793343718761	37.0	35.0	37.0	26.0	37.0
6	32.86884280260139	37.0	35.0	37.0	25.0	37.0
7	32.767591747473986	37.0	33.0	37.0	25.0	37.0
8	32.68709153130154	37.0	33.0	37.0	25.0	37.0
9	33.97319919204119	38.0	34.0	39.0	23.0	39.0
10-11	33.947770049708225	38.0	34.0	39.0	23.5	39.0
12-13	33.95192765200125	38.0	34.0	39.0	23.0	39.0
14-15	34.97461746627596	39.0	34.0	41.0	23.5	41.0
16-17	34.88467829194032	39.0	34.0	41.0	23.0	41.0
18-19	34.96969868535129	39.0	34.0	41.0	23.5	41.0
20-21	35.01013780135468	39.0	34.0	41.0	24.0	41.0
22-23	34.98491596725159	39.0	34.0	41.0	24.0	41.0
24-25	34.89858938902118	39.0	34.0	41.0	23.5	41.0
26-27	34.82874996282749	39.0	34.0	41.0	23.5	41.0
28-29	34.67700748711543	39.0	34.0	41.0	22.5	41.0
30-31	34.56431187392914	39.0	34.0	41.0	22.0	41.0
32-33	34.444691308037726	39.0	34.0	41.0	21.5	41.0
34-35	34.331326017783326	39.0	34.0	41.0	19.5	41.0
36-37	34.176835349799035	39.0	33.0	41.0	18.5	41.0
38-39	34.00162243709839	39.0	33.0	41.0	16.5	41.0
40-41	33.87803470654305	38.5	33.0	41.0	16.0	41.0
42-43	33.7640231864962	38.0	33.0	41.0	15.0	41.0
44-45	33.578176934285864	38.0	33.0	40.0	13.5	41.0
46-47	33.32819980967675	38.0	33.0	40.0	9.0	41.0
48-49	33.16928275355655	38.0	32.0	40.0	8.5	41.0
50-51	32.99909070321239	38.0	32.0	40.0	7.5	41.0
52-53	32.79913262236619	38.0	32.0	40.0	4.5	41.0
54-55	32.54419496924403	38.0	31.0	40.0	2.0	41.0
56-57	32.276385905556666	37.0	31.0	40.0	2.0	41.0
58-59	32.04034933009417	37.0	31.0	40.0	2.0	41.0
60-61	31.735489567677988	36.0	30.5	40.0	2.0	41.0
62-63	31.44342058007416	36.0	30.0	39.0	2.0	41.0
64-65	31.13138566536506	35.5	30.0	39.0	2.0	41.0
66-67	30.845877339866544	35.0	30.0	39.0	2.0	40.0
68-69	30.478277814759657	35.0	29.0	38.0	2.0	40.0
70-71	30.149895001955848	35.0	29.0	37.5	2.0	40.0
72-73	29.784123220580533	35.0	29.0	37.0	2.0	39.0
74-75	29.72060540865742	35.0	29.0	37.0	2.0	39.0
76-77	29.456265683939876	34.5	29.0	36.0	2.0	39.0
78-79	29.19219445912282	34.0	29.0	36.0	2.0	38.5
80-81	28.849456766655	34.0	29.0	35.0	2.0	37.0
82-83	28.590526499996567	34.0	29.0	35.0	2.0	37.0
84-85	28.386387083639292	34.0	28.5	35.0	2.0	36.0
86-87	28.163858426493363	34.0	28.0	35.0	2.0	36.0
88-89	27.99035802274271	34.0	27.5	35.0	2.0	36.0
90-91	27.828205242582083	34.0	27.0	35.0	2.0	35.0
92-93	27.995468099123645	34.0	28.5	35.0	2.0	35.0
94-95	27.97017992638699	34.0	29.0	35.0	2.0	35.0
96-97	27.8994023232247	34.0	28.5	35.0	2.0	35.0
98-99	27.786056191110166	34.0	28.0	35.0	2.0	35.0
100-101	27.659802619689764	34.0	27.0	35.0	2.0	35.0
102-103	26.324316712074317	32.0	24.5	34.0	2.0	35.0
104-105	27.330557118707265	34.0	26.5	35.0	2.0	35.0
106-107	27.48614723516588	34.0	27.0	35.0	2.0	35.0
108-109	27.41935080784443	34.0	27.0	35.0	2.0	35.0
110-111	27.38005488950042	34.0	27.0	35.0	2.0	35.0
112-113	27.282558543844118	34.0	27.0	35.0	2.0	35.0
114-115	27.13306872224929	34.0	26.5	35.0	2.0	35.0
116-117	26.947596768622283	34.0	25.0	35.0	2.0	35.0
118-119	26.802252539740273	34.0	25.0	35.0	2.0	35.0
120-121	26.591280816010944	34.0	25.0	35.0	2.0	35.0
122-123	26.398250261351343	34.0	25.0	35.0	2.0	35.0
124-125	25.299906668405196	33.0	21.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78029.0
3	11363.0
4	7496.0
5	7612.0
6	10327.0
7	10766.0
8	9808.0
9	10473.0
10	11018.0
11	11798.0
12	11929.0
13	12514.0
14	13078.0
15	11843.0
16	9463.0
17	8500.0
18	7678.0
19	7547.0
20	7233.0
21	7964.0
22	9477.0
23	12503.0
24	17092.0
25	21745.0
26	24863.0
27	28185.0
28	32765.0
29	38668.0
30	45790.0
31	55017.0
32	67851.0
33	85924.0
34	113505.0
35	162395.0
36	268173.0
37	382756.0
38	115362.0
39	94.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.8715200681222	18.706593844280345	13.029457815053725	24.392428272543732
2	15.690586437405345	20.372374254771067	39.33819324020736	24.59884606761623
3	19.52729431737323	24.304572046976563	29.051419798704554	27.116713836945657
4	12.947137161052602	16.514510812398438	36.08481757026901	34.453534456279954
5	13.696905709412816	38.21913859507242	33.87539563703998	14.208560058474784
6	32.80818548894456	35.99184425151276	17.04637223613923	14.153598023403452
7	28.270551382674686	32.96611473303063	20.365361727097977	18.39797215719671
8	25.74495087422096	36.00574444757734	19.580250401636633	18.669054276565067
9	26.258546586941417	17.232092559071894	19.181071829934716	37.328289024051976
10-11	24.781855103116413	26.921052037062825	27.151200515431928	21.14589234438883
12-13	25.23527591695411	24.59483356677432	27.064351359095536	23.105539157176036
14-15	23.0470968468842	25.942042745019762	24.77072694215084	26.240133465945192
16-17	22.76254014754679	28.75932748587347	25.686336807854293	22.791795558725447
18-19	22.617739693004907	27.95332219614903	27.320778931848324	22.10815917899773
20-21	23.818110011264743	27.021026089647222	26.922019897899506	22.238844001188532
22-23	23.414600808166192	26.782550907864422	26.500484836804016	23.30236344716537
24-25	23.029421949664908	26.96967729962372	26.725530963949097	23.275369786762283
26-27	22.967905057143177	27.178116223960874	26.750732679971623	23.10324603892433
28-29	22.982035914444534	27.220259512374795	26.892451698177737	22.905252875002933
30-31	23.365565676914958	27.171022060305887	26.72627005108657	22.737142211692575
32-33	22.93638765612847	27.207387793384267	26.692478382211647	23.16374616827561
34-35	22.906097340225813	27.374733017420034	26.775267940557363	22.943901701796793
36-37	23.26668912415234	27.06228063201999	26.661734789295217	23.009295454532456
38-39	23.069673225714396	27.342547683058854	26.598456916681396	22.98932217454536
40-41	23.1815066309387	27.24087811648686	26.632614341837265	22.945000910737175
42-43	23.200937672729	27.08150506881423	26.620875342312594	23.096681916144174
44-45	23.218471790361146	27.16223952271829	26.49678108213059	23.122507604789973
46-47	23.07930748037294	27.18069477865136	26.535328709482325	23.20466903149337
48-49	23.003212063381135	27.180438582620205	26.62306709303546	23.19328226096321
50-51	22.891142845704678	27.35211863679777	26.515158447381516	23.24158007011604
52-53	22.89319930821811	27.28323481236789	26.29743418463988	23.52613169477412
54-55	23.02072198974109	27.109501758335146	26.2955890351261	23.57418721679766
56-57	23.037121402102915	27.111413961151758	26.191635638993727	23.659828997751596
58-59	22.923016521847792	26.851612766708215	26.53895612026672	23.68641459117727
60-61	23.20532404852335	26.804855995273712	26.458820293059443	23.530999663143497
62-63	22.716965550529835	26.872611250050753	26.589406946626397	23.82101625279301
64-65	22.741696436715973	26.80016459432047	26.603028867201605	23.85511010176195
66-67	22.979413324793082	26.804626032800854	26.327039187456126	23.888921454949937
68-69	23.178315964353676	26.621734648505136	26.37098653612171	23.82896285101948
70-71	22.83595832046575	26.438595889235838	26.6075615642407	24.117884226057715
72-73	22.95206752700557	26.444426588629767	26.61828249239595	23.985223391968717
74-75	22.971610858794325	26.524592760899097	26.50014426045002	24.003652119856557
76-77	22.779053897362623	26.395687684847108	26.845082577312308	23.980175840477965
78-79	22.980991359084797	26.431981708790374	26.759072045943057	23.82795488618177
80-81	22.8243256110669	26.378966276764604	26.768305986241003	24.028402125927492
82-83	22.79411701630302	26.407173728734808	26.710759020417736	24.087950234544444
84-85	23.039090515788242	26.38637695224539	26.46275253482009	24.111779997146282
86-87	22.861029235993634	26.177743540825276	26.72752685294161	24.23370037023947
88-89	22.934580957152107	26.168017458498323	26.69223563482641	24.205165949523163
90-91	23.026139331610423	26.166896661369066	26.683855744425085	24.123108262595427
92-93	22.761396539704528	26.144229114040602	26.904875330196692	24.189499016058182
94-95	22.59469620608969	26.28995495483903	26.83196665176885	24.28338218730243
96-97	22.6377069027735	26.191690776609917	26.868322822942183	24.30227949767441
98-99	22.672471007130657	26.133514890540965	26.705780387646747	24.488233714681627
100-101	22.484126428121066	26.36432653306321	26.80496916788313	24.3465778709326
102-103	22.401464285173027	26.29193685227299	26.821542103202823	24.48505675935116
104-105	22.335567092115028	26.385356989158144	26.751546640854766	24.527529277872063
106-107	22.122471365832762	26.569538976610545	26.81168620601502	24.496303451541674
108-109	22.10493986208845	26.577613923730276	26.813166826761808	24.504279387419466
110-111	21.972922079129066	26.73579367133312	26.651605901722792	24.63967834781502
112-113	21.958806221101305	26.873722166462212	26.59254874711549	24.574922865320993
114-115	21.85273376499524	26.838254322886268	26.660760697586838	24.64825121453166
116-117	21.906297038490923	26.64940682890228	26.64491744101459	24.799378691592207
118-119	21.895449599890195	26.543500235190503	26.664055027429363	24.896995137489938
120-121	21.9052039081927	26.623933676935852	26.638345766620226	24.832516648251218
122-123	21.886377888069532	26.582378018459966	26.55098137389772	24.980262719572778
124-125	21.678158176171095	26.61262466306024	26.60203604765335	25.107181113115317
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1266.0
1	829.0
2	342.0
3	245.0
4	180.0
5	152.0
6	155.0
7	185.5
8	234.0
9	277.0
10	281.5
11	270.5
12	288.0
13	335.0
14	396.5
15	462.5
16	549.0
17	683.5
18	812.5
19	938.0
20	1119.0
21	1361.0
22	1690.0
23	2202.5
24	2873.5
25	3656.5
26	4962.5
27	6824.5
28	8683.0
29	11160.5
30	14014.0
31	16997.5
32	20861.5
33	25054.0
34	29043.5
35	32570.0
36	37251.0
37	41171.5
38	43680.0
39	47115.5
40	50801.0
41	53878.5
42	56558.0
43	59824.5
44	62859.5
45	65614.5
46	68078.5
47	70058.0
48	72085.0
49	74579.5
50	77785.0
51	77326.0
52	71703.0
53	67774.5
54	65220.5
55	60423.0
56	54630.5
57	48883.5
58	44678.5
59	40194.5
60	33831.5
61	27409.5
62	21687.0
63	16155.0
64	11745.0
65	8961.0
66	6646.0
67	4813.5
68	3491.0
69	2557.5
70	1933.5
71	1450.5
72	1075.0
73	901.0
74	723.5
75	504.0
76	358.0
77	232.5
78	158.5
79	111.5
80	75.5
81	50.0
82	35.5
83	29.5
84	25.5
85	18.0
86	14.0
87	11.0
88	9.0
89	10.5
90	9.0
91	7.0
92	8.0
93	8.0
94	6.0
95	5.5
96	6.0
97	4.5
98	5.0
99	7.0
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06610987965256857
2	0.008463894626799435
3	0.018757820524258208
4	0.016413092958725933
5	0.009035779398880478
6	0.00823514071796702
7	0.008292329195175124
8	0.007606067468677871
9	0.005661659243602324
10-11	0.00957906993235747
12-13	0.0071485596510130365
14-15	0.008177952240758915
16-17	0.01198098597509785
18-19	0.00611916706126716
20-21	0.01306756704205183
22-23	0.0044321069836280825
24-25	0.0035742798255065183
26-27	0.003288337439465997
28-29	0.009779229602585834
30-31	0.005804630436622586
32-33	0.002516292997156589
34-35	0.007348719321241402
36-37	0.006576674878931994
38-39	0.0015440888846188158
40-41	0.001172363782766138
42-43	0.0011151753055580337
44-45	0.00200159670228365
46-47	0.0035170913482984135
48-49	0.0022589448497201195
50-51	0.004231947313399717
52-53	0.004346324267815927
54-55	0.004289135790607822
56-57	0.0017442485548471811
58-59	0.002316133326928224
60-61	0.004861020562688865
62-63	0.0024591045199484846
64-65	0.0035742798255065183
66-67	0.0012295522599742423
68-69	0.0020873794180958066
70-71	0.001372523452994503
72-73	0.0016870600776390766
74-75	0.0017442485548471811
76-77	0.0017442485548471811
78-79	2.5734814743646935E-4
80-81	1.4297119302026073E-4
82-83	8.578271581215644E-5
84-85	4.861020562688865E-4
86-87	3.4313086324862577E-4
88-89	0.0
90-91	7.434502037053559E-4
92-93	0.0014297119302026074
94-95	0.0014583061688066595
96-97	9.72204112537773E-4
98-99	0.001572683123222868
100-101	0.0012295522599742423
102-103	0.002144567895303911
104-105	0.003231148962257893
106-107	0.004632266653856448
108-109	0.009407504500733155
110-111	0.0075202847528657146
112-113	0.0022303506111160673
114-115	0.005175557187333439
116-117	0.002058785179491755
118-119	0.0012295522599742423
120-121	0.00411757035898351
122-123	5.432905334769909E-4
124-125	0.08260875532710665
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1748604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.412606482852475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.10255219929184	34.14537218784379
2	14.253473313545003	14.086025357120771
3	6.291404964212467	9.326241531626856
4	3.277027146726827	6.477058113393502
5	1.948100474524769	4.813036106837529
6	1.278391144237481	3.7901183124822087
7	0.8420808374720683	2.9126586334130717
8	0.5944769806657382	2.349972568700034
9	0.4356237601488088	1.937277489133224
>10	1.8903877912103542	15.29135520123854
>50	0.06724455069749866	2.2611880420715753
>100	0.017138626370400575	1.435599919553483
>500	0.0011656727204370284	0.3966262142330633
>1k	9.325381763496227E-4	0.7774703223522929
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	3059	0.17493955177959103	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2852	0.16310153699751345	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1758	0.10053734293184735	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	5.718847720810429E-5	0.0
12-13	0.0	0.0	0.0	5.718847720810429E-5	0.0
14-15	0.0	0.0	0.0	1.1437695441620859E-4	0.0
16-17	0.0	0.0	0.0	2.573481474364693E-4	0.0
18-19	0.0	0.0	0.0	3.4313086324862577E-4	0.0
20-21	0.0	0.0	0.0	4.289135790607822E-4	0.0
22-23	0.0	0.0	0.0	8.578271581215644E-4	0.0
24-25	0.0	0.0	0.0	0.0024019160427403803	0.0
26-27	0.0	0.0	0.0	0.003917410688755144	0.0
28-29	0.0	0.0	0.0	0.009664852648169625	0.0
30-31	0.0	0.0	0.0	0.02590638017527125	0.0
32-33	0.0	0.0	0.0	0.05724566568531239	0.0
34-35	0.0	0.0	0.0	0.0979352672188786	0.0
36-37	0.0	0.0	0.0	0.1660467435737308	0.0
38-39	0.0	0.0	0.0	0.2656976651088525	5.718847720810429E-5
40-41	0.0	0.0	0.0	0.37727238414186404	5.718847720810429E-5
42-43	0.0	0.0	0.0	0.4775523789262749	5.718847720810429E-5
44-45	0.0	0.0	0.0	0.5918721448652754	5.718847720810429E-5
46-47	0.0	0.0	0.0	0.7262078778271124	5.718847720810429E-5
48-49	0.0	0.0	0.0	0.8671202856678814	5.718847720810429E-5
50-51	0.0	0.0	5.718847720810429E-5	1.002971513275733	5.718847720810429E-5
52-53	0.0	0.0	5.718847720810429E-5	1.1432262536286089	5.718847720810429E-5
54-55	0.0	0.0	5.718847720810429E-5	1.3036685264359456	5.718847720810429E-5
56-57	0.0	0.0	5.718847720810429E-5	1.4921903415524613	5.718847720810429E-5
58-59	0.0	0.0	5.718847720810429E-5	1.6936939409952168	5.718847720810429E-5
60-61	0.0	0.0	5.718847720810429E-5	1.9013167074992392	5.718847720810429E-5
62-63	0.0	0.0	5.718847720810429E-5	2.1047647151670703	5.718847720810429E-5
64-65	0.0	0.0	5.718847720810429E-5	2.3329753334660106	5.718847720810429E-5
66-67	0.0	0.0	5.718847720810429E-5	2.595327472658189	5.718847720810429E-5
68-69	0.0	0.0	5.718847720810429E-5	2.8889617088832003	5.718847720810429E-5
70-71	0.0	0.0	5.718847720810429E-5	3.2208264421218296	5.718847720810429E-5
72-73	0.0	0.0	5.718847720810429E-5	3.5425688148946244	5.718847720810429E-5
74-75	0.0	0.0	5.718847720810429E-5	3.875377158007187	5.718847720810429E-5
76-77	0.0	0.0	5.718847720810429E-5	4.254107848317858	5.718847720810429E-5
78-79	0.0	0.0	5.718847720810429E-5	4.674643315467653	5.718847720810429E-5
80-81	2.8594238604052146E-5	0.0	5.718847720810429E-5	5.121742830280612	5.718847720810429E-5
82-83	5.718847720810429E-5	0.0	5.718847720810429E-5	5.5881434561513075	5.718847720810429E-5
84-85	5.718847720810429E-5	0.0	5.718847720810429E-5	6.062464686115324	5.718847720810429E-5
86-87	5.718847720810429E-5	0.0	5.718847720810429E-5	6.587740849271762	5.718847720810429E-5
88-89	5.718847720810429E-5	0.0	5.718847720810429E-5	7.171720984282318	5.718847720810429E-5
90-91	5.718847720810429E-5	0.0	5.718847720810429E-5	7.770941848468835	5.718847720810429E-5
92-93	5.718847720810429E-5	0.0	5.718847720810429E-5	8.391265260745143	5.718847720810429E-5
94-95	5.718847720810429E-5	0.0	5.718847720810429E-5	9.033263105883321	5.718847720810429E-5
96-97	5.718847720810429E-5	0.0	5.718847720810429E-5	9.704798799499486	5.718847720810429E-5
98-99	5.718847720810429E-5	0.0	5.718847720810429E-5	10.42417265430023	5.718847720810429E-5
100-101	5.718847720810429E-5	0.0	5.718847720810429E-5	11.21486054017948	5.718847720810429E-5
102-103	5.718847720810429E-5	0.0	5.718847720810429E-5	12.003661206310863	5.718847720810429E-5
104-105	5.718847720810429E-5	0.0	5.718847720810429E-5	12.783340310327553	5.718847720810429E-5
106-107	5.718847720810429E-5	5.718847720810429E-5	5.718847720810429E-5	13.588067967361393	5.718847720810429E-5
108-109	5.718847720810429E-5	8.578271581215644E-5	5.718847720810429E-5	14.42770918973078	5.718847720810429E-5
110-111	5.718847720810429E-5	1.1437695441620859E-4	5.718847720810429E-5	15.315588892625204	5.718847720810429E-5
112-113	5.718847720810429E-5	1.1437695441620859E-4	5.718847720810429E-5	16.226201015209845	5.718847720810429E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	4665	0.0	24.870916	4
GTATAGG	475	0.0	22.559847	1
TACATGG	11525	0.0	21.685371	2
GTACATG	11890	0.0	21.529991	1
GTATCAA	8690	0.0	20.620728	1
ACATGGG	11880	0.0	20.586565	3
GTATAAT	1075	0.0	20.490406	1
CGTTAGA	155	0.005671169	19.204172	1
AACGCAG	9435	0.0	18.792978	6
GTATTAG	545	0.0	18.569906	1
TCAACGC	9590	0.0	18.42666	4
GTACAAG	1300	0.0	18.317825	1
TATCAAC	9650	0.0	18.252523	2
TCTATAC	490	1.6370905E-11	18.216026	3
CAACGCA	9800	0.0	18.032835	5
CTATTCG	165	0.008160661	18.030481	9
ATCAACG	9810	0.0	17.954828	3
TCGCACT	200	0.0011748136	17.849665	4
ATGGGGA	2235	0.0	17.837376	5
GAGTACT	2365	0.0	17.487873	12-13
>>END_MODULE