FastQCFastQC Report
Mon 15 Aug 2016
SRR2921146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748604
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36630.20948139201328603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31390.17951462995623937No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28490.16292997156588915No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27620.15795457404878407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20960.11986704822818658No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95500.030.6481841
GGTATCA72200.026.9983521
TATCAAC115950.025.028542
TCAACGC118200.024.4022664
ATCAACG120000.024.3341983
CAACGCA118700.024.2730525
GTATAGA5450.024.063171
AACGCAG121250.023.846436
GAGTACT60350.023.1181512-13
AGTACTT64200.022.47316412-13
CATGGGG47950.022.122474
GTACATG111450.021.8226131
GTACTTT63750.021.79216614-15
ACGCAGA133100.021.5892687
TACATGG108000.021.4636332
CGCAGAG134500.021.187018
ACATGGG110900.020.3661963
GCAGAGT140050.019.5833369
GTATAGG4551.05501385E-1018.3418561
AGAGTAC130400.018.06779310-11
Produced by FastQC (version 0.11.5)