##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921146_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1748604
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.455375259349744	31.0	30.0	34.0	27.0	34.0
2	30.51835406987517	31.0	30.0	34.0	27.0	34.0
3	30.611166965190517	31.0	30.0	34.0	27.0	34.0
4	34.11788718314724	37.0	35.0	37.0	30.0	37.0
5	33.75640968452549	37.0	35.0	37.0	28.0	37.0
6	33.610991968450264	37.0	35.0	37.0	28.0	37.0
7	33.424881219532836	37.0	35.0	37.0	27.0	37.0
8	33.39483210606861	37.0	35.0	37.0	27.0	37.0
9	34.77134960231133	39.0	35.0	39.0	27.0	39.0
10-11	34.73011813995622	38.5	35.0	39.0	27.0	39.0
12-13	34.59789437745768	38.0	35.0	39.0	27.0	39.0
14-15	35.63242563782309	39.0	35.0	41.0	27.0	41.0
16-17	35.57519855839287	39.0	35.0	41.0	27.0	41.0
18-19	35.64544173523565	39.0	35.0	41.0	27.0	41.0
20-21	35.65505654796627	39.0	35.0	41.0	27.0	41.0
22-23	35.63335209115385	39.0	35.0	41.0	27.0	41.0
24-25	35.543539589295236	39.0	35.0	41.0	26.5	41.0
26-27	35.42131809146039	39.0	35.0	41.0	26.0	41.0
28-29	35.31324788231069	39.0	35.0	41.0	25.0	41.0
30-31	35.19841856703977	39.0	35.0	41.0	25.0	41.0
32-33	35.02216796942018	39.0	34.0	41.0	25.0	41.0
34-35	34.87483901443666	39.0	34.0	41.0	24.0	41.0
36-37	34.710839904289365	39.0	34.0	41.0	24.0	41.0
38-39	34.52973686437867	39.0	34.0	41.0	23.0	41.0
40-41	34.359582558429466	39.0	33.5	41.0	21.5	41.0
42-43	34.19318753702954	38.5	33.0	41.0	20.0	41.0
44-45	33.97838532909681	38.0	33.0	41.0	18.5	41.0
46-47	33.69802396654703	38.0	33.0	41.0	15.0	41.0
48-49	33.442658543615366	38.0	33.0	40.0	11.0	41.0
50-51	33.215967995040614	38.0	32.5	40.0	8.5	41.0
52-53	33.037024392029295	38.0	32.0	40.0	8.0	41.0
54-55	32.827391736493794	38.0	32.0	40.0	6.5	41.0
56-57	32.55141873174258	37.5	31.5	40.0	2.0	41.0
58-59	32.32739545374481	37.0	31.0	40.0	2.0	41.0
60-61	32.056376686774136	36.5	31.0	40.0	2.0	41.0
62-63	31.76012121669629	36.0	31.0	39.5	2.0	41.0
64-65	31.475406953203812	36.0	31.0	39.0	2.0	41.0
66-67	31.1393242838287	35.0	30.0	39.0	2.0	41.0
68-69	30.775998453623576	35.0	30.0	38.5	2.0	40.0
70-71	30.405980999700333	35.0	29.5	37.5	2.0	40.0
72-73	30.209629796111642	35.0	29.5	37.0	2.0	39.5
74-75	30.01538598790807	35.0	30.0	37.0	2.0	39.0
76-77	29.77072510414022	35.0	29.0	36.0	2.0	39.0
78-79	29.44412714371007	35.0	29.0	36.0	2.0	38.5
80-81	29.150440294086025	34.0	29.0	35.5	2.0	37.0
82-83	28.928461218206067	34.0	29.0	35.0	2.0	37.0
84-85	28.758543672552506	34.0	29.0	35.0	2.0	36.5
86-87	28.549091446662594	34.0	29.0	35.0	2.0	36.0
88-89	28.395356238462224	34.0	29.0	35.0	2.0	36.0
90-91	28.237282426438462	34.0	29.0	35.0	2.0	35.5
92-93	28.10801816763544	34.0	29.0	35.0	2.0	35.0
94-95	28.003244016369628	34.0	28.0	35.0	2.0	35.0
96-97	27.929501476606482	34.0	28.0	35.0	2.0	35.0
98-99	27.88923049472608	34.0	28.0	35.0	2.0	35.0
100-101	27.73214061045268	34.0	27.0	35.0	2.0	35.0
102-103	26.375322543011453	31.5	24.5	34.0	2.0	35.0
104-105	27.454258368389873	33.5	27.0	35.0	2.0	35.0
106-107	27.72318003390133	34.0	27.5	35.0	2.0	35.0
108-109	27.91706927354621	34.0	29.0	35.0	2.0	35.0
110-111	27.89481838083408	34.0	29.0	35.0	2.0	35.0
112-113	27.811955708668172	34.0	29.0	35.0	2.0	35.0
114-115	27.685779341692	34.0	29.0	35.0	2.0	35.0
116-117	27.574899748599453	34.0	28.5	35.0	2.0	35.0
118-119	27.424257579188883	34.0	27.0	35.0	2.0	35.0
120-121	27.2475932229367	34.0	27.0	35.0	2.0	35.0
122-123	27.050762493966616	34.0	27.0	35.0	2.0	35.0
124-125	26.24391285848597	33.5	25.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47235.0
3	15734.0
4	7926.0
5	6519.0
6	7763.0
7	9320.0
8	10318.0
9	11210.0
10	12224.0
11	13215.0
12	14490.0
13	15935.0
14	18238.0
15	14956.0
16	10195.0
17	9018.0
18	8307.0
19	7998.0
20	7884.0
21	8096.0
22	8791.0
23	10494.0
24	14241.0
25	18914.0
26	23244.0
27	27289.0
28	32352.0
29	38165.0
30	45244.0
31	54612.0
32	66692.0
33	84327.0
34	109861.0
35	156267.0
36	264109.0
37	401439.0
38	135765.0
39	217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.54512426700609	19.224042314767015	13.849764720829905	24.38106869739699
2	16.001077432759104	20.520919294910616	38.71088944819315	24.767113824137123
3	19.326380660008326	24.357365731357486	29.87783257767428	26.43842103095991
4	13.256189275264841	16.93666896021092	35.80031613098754	34.0068256335367
5	13.524066444893739	37.604302997597046	34.46541298006848	14.406217577440739
6	32.55775780470298	36.29800996874401	16.738510636472565	14.405721590080441
7	27.923914479573046	32.746778342039384	20.55558192885957	18.773725249528
8	26.272436467542153	35.25651767836073	19.548774133697698	18.92227172039942
9	26.372385612129683	15.903794873437487	19.43647164741184	38.28734786702099
10-11	25.144832102864612	26.13263895144904	27.371715949763132	21.350812995923224
12-13	25.914226693500435	23.61641588416597	27.549162912770132	22.920194509563462
14-15	23.376063609192443	24.977991333819556	25.49962103336336	26.14632402362464
16-17	22.732124592999707	27.9937831635163	26.37075867371104	22.90333356977295
18-19	22.437681686670075	26.841720808007313	28.660532364108953	22.06006514121366
20-21	23.52347946163828	25.842266320129987	28.48402215935596	22.15023205887577
22-23	23.063686996803163	25.727181903233475	28.153052209053588	23.056078890909774
24-25	22.764971976345496	26.01025875117123	28.37041791225776	22.854351360225518
26-27	22.683748104093727	26.12625341463338	28.496411542440413	22.693586938832482
28-29	22.60511402475613	26.05228429909397	28.671533080536083	22.67106859561382
30-31	23.08701279200303	25.969324934037886	28.38803600923827	22.555626264720814
32-33	22.792895394052685	26.07749720000595	28.304393388009963	22.8252140179314
34-35	22.717224640763032	26.283953438552963	28.41178948789444	22.58703243278956
36-37	23.052496968903046	25.928914305261706	28.28795006319612	22.730638662639123
38-39	22.92387825325862	26.154640443235483	28.168507283809717	22.752974019696175
40-41	23.0084827932982	26.08777851952404	28.178158680690817	22.725580006486943
42-43	23.180615349061235	25.89400427653068	28.00918801976494	22.916192354643147
44-45	23.121502683445012	26.1098456289764	27.778203630630742	22.99044805694784
46-47	23.136510235579063	26.12575352464765	27.582291355617873	23.15544488415542
48-49	23.137781835321682	26.262939141417608	27.447110830438902	23.152168192821808
50-51	23.046615680274765	26.499852555340368	27.2875874478402	23.165944316544664
52-53	23.0792222159934	26.510886390495987	27.04625357378617	23.36363781972444
54-55	23.00131342750807	26.488331889859722	26.993852747386015	23.516501935246193
56-57	23.040167674686394	26.48012438313254	26.825976305196303	23.653731636984762
58-59	22.809790329394126	26.387510085405257	27.183445549107947	23.619254036092666
60-61	23.165807055192175	26.45329539659536	27.033529566716346	23.347367981496117
62-63	22.69656680250877	26.55224963217376	27.142401956858503	23.608781608458973
64-65	22.61365622347931	26.461267741600548	27.233129801255174	23.69194623366497
66-67	22.843411732535802	26.446830833231317	26.9335126557296	23.77624477850328
68-69	23.030565994455234	26.30556956254249	26.95526176317568	23.708602679826594
70-71	22.79116971322392	26.04752102994078	27.143126024036835	24.01818323279847
72-73	22.89264292955529	26.079451967019164	27.089364297303543	23.938540806122006
74-75	22.863298648679955	26.04832627313156	26.981068019616977	24.107307058571504
76-77	22.666764666516425	26.034518063812772	27.21901021677033	24.07970705290047
78-79	22.87374823321201	26.064720354141247	27.128797199909165	23.93273421273758
80-81	22.67592880191146	26.03576950599848	27.057681846340763	24.230619845749295
82-83	22.560429056742137	26.068251642969052	26.96880289347886	24.402516406809948
84-85	22.716021701908485	25.972880908046015	26.755515491823463	24.55558189822204
86-87	22.5686761528485	25.795608100861823	27.08566591309147	24.550049833198205
88-89	22.508814893387623	25.787706423225277	27.04342655904713	24.660052124339973
90-91	22.625929433015475	25.85779242302783	26.99806470303759	24.518213440919105
92-93	22.43504199926212	25.805994011136885	27.053959828284935	24.70500416131606
94-95	22.325219453407012	25.8228323059667	26.969618279480713	24.88232996114558
96-97	22.381168920310248	25.74790651383074	26.98712448806827	24.883800077790745
98-99	22.414990801198062	25.607502721890175	26.816745508773966	25.160760968137797
100-101	22.240277707471968	25.804110187268936	26.88783968788224	25.067772417376847
102-103	22.238901325926783	25.556091930618486	26.938442954033547	25.26656378942118
104-105	22.084120996502346	25.671575202409176	26.86681175875057	25.377492042337906
106-107	21.916637639181786	25.75072511515189	26.941223775883792	25.39141346978253
108-109	21.93309804677457	25.762820052683523	26.916612639984393	25.38746926055751
110-111	21.739272179446555	25.854210540163052	26.78909493147441	25.61742234891598
112-113	21.60527808098483	25.978494525257823	26.71340742039254	25.702819973364804
114-115	21.539718471611526	25.89287348380514	26.77708217993475	25.790325864648583
116-117	21.58313539803725	25.725707148033106	26.755695131792596	25.93546232213705
118-119	21.461828068604188	25.709813368197043	26.81231530840601	26.01604325479276
120-121	21.43081002065714	25.835779172204592	26.786653840786755	25.946756966351508
122-123	21.34429217581084	25.83530337610092	26.59950934568081	26.220895102407432
124-125	21.06558720762068	25.882670339375586	26.717784381507787	26.333958071495942
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	77.0
1	75.0
2	116.0
3	187.5
4	164.5
5	123.0
6	181.0
7	395.5
8	681.5
9	790.5
10	666.5
11	486.5
12	435.5
13	492.0
14	569.5
15	675.5
16	790.0
17	917.5
18	1018.5
19	1177.0
20	1405.5
21	1652.5
22	2029.5
23	2541.5
24	3169.5
25	3905.5
26	5193.5
27	7130.5
28	9068.5
29	11438.5
30	14219.0
31	17138.5
32	20887.0
33	24974.5
34	29085.5
35	32693.5
36	37031.5
37	40851.5
38	43391.5
39	46806.0
40	50370.0
41	53459.0
42	55974.5
43	59272.5
44	62846.0
45	65583.0
46	67901.5
47	69922.0
48	71995.5
49	74637.0
50	78773.0
51	78313.5
52	72169.0
53	68223.5
54	65364.5
55	60857.5
56	55135.5
57	49015.0
58	44419.5
59	39943.0
60	33524.5
61	26994.0
62	21179.0
63	15716.0
64	11485.5
65	8564.0
66	6218.0
67	4445.5
68	3207.0
69	2290.0
70	1674.5
71	1209.0
72	912.0
73	747.0
74	602.5
75	406.5
76	262.5
77	166.5
78	90.5
79	47.5
80	25.5
81	13.5
82	9.0
83	4.5
84	2.0
85	4.0
86	3.0
87	1.5
88	2.0
89	2.0
90	3.0
91	4.5
92	2.5
93	1.5
94	3.0
95	2.5
96	2.5
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07537441296028145
2	1.7156543162431288E-4
3	0.0011437695441620858
4	0.17871399127532592
5	0.06696770681069013
6	0.007949198331926496
7	0.011609260873245172
8	0.009550475693753417
9	0.01704216620801508
10-11	0.01638449872012188
12-13	0.017699833695908277
14-15	0.02453385672227674
16-17	0.025620437789230725
18-19	0.022989767837657927
20-21	0.025649032027834775
22-23	0.026792801571996865
24-25	0.025048553017149682
26-27	0.024562450960880795
28-29	0.02464823367669295
30-31	0.025248712687378043
32-33	0.022074752202328258
34-35	0.02399056618879975
36-37	0.027135932435245487
38-39	0.031396473987249256
40-41	0.02727890362826575
42-43	0.026192322561311765
44-45	0.02733609210547385
46-47	0.027793599923138686
48-49	0.024019160427403805
50-51	0.028050948070575153
52-53	0.026192322561311765
54-55	0.02893736946730077
56-57	0.02613513408410366
58-59	0.02341868141671871
60-61	0.024619639438088897
62-63	0.02836548469521973
64-65	0.03193976452072625
66-67	0.030996154646792527
68-69	0.026792801571996865
70-71	0.03545685586902466
72-73	0.03888816450151092
74-75	0.02427650857484027
76-77	0.0172709201168475
78-79	0.02144567895303911
80-81	0.023504464132530864
82-83	0.022703825451617404
84-85	0.015926990902457045
86-87	0.015526671562000315
88-89	0.01627012176570567
90-91	0.01678481806057861
92-93	0.02053066331770944
94-95	0.016184339049893515
96-97	0.01852906661542579
98-99	0.015926990902457045
100-101	0.016556064151746194
102-103	0.01383961148436124
104-105	0.01652746991314214
106-107	0.01492619255131522
108-109	0.013696640291340979
110-111	0.011780826304869483
112-113	0.00946469297794126
114-115	0.008463894626799435
116-117	0.00783482137751029
118-119	0.004632266653856448
120-121	0.0032025547236538407
122-123	0.007005588457992776
124-125	0.05367138585980588
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1748604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.448946230880466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.65399883819116	33.262138982464535
2	14.362829707197749	13.917279284146328
3	6.351746375421068	9.232062558448957
4	3.3886111991242287	6.566985671349165
5	2.0233775229203648	4.901525440637043
6	1.2787906739586063	3.717363636190315
7	0.8475774183011092	2.874496293604525
8	0.6240565722950021	2.41879066529185
9	0.4457054289778393	1.9434562527022963
>10	1.9291547935189997	15.422820794378714
>50	0.07075160549331783	2.3371014022567125
>100	0.02102457179776991	1.8072240990646513
>500	0.0011876464014347447	0.40778751535893815
>1k	0.0011876464014347447	1.1909674041060112
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3663	0.20948139201328603	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3139	0.17951462995623937	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2849	0.16292997156588915	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2762	0.15795457404878407	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2096	0.11986704822818658	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	5.718847720810429E-5	0.0
16-17	0.0	0.0	0.0	1.7156543162431288E-4	0.0
18-19	0.0	0.0	0.0	2.2875390883241717E-4	0.0
20-21	0.0	2.8594238604052146E-5	0.0	3.717251018526779E-4	0.0
22-23	0.0	5.718847720810429E-5	0.0	8.006386809134601E-4	0.0
24-25	0.0	5.718847720810429E-5	0.0	0.002344727565532276	0.0
26-27	0.0	5.718847720810429E-5	0.0	0.003946004927359196	0.0
28-29	0.0	5.718847720810429E-5	0.0	0.009693446886773678	0.0
30-31	0.0	5.718847720810429E-5	0.0	0.025706220505042877	0.0
32-33	0.0	5.718847720810429E-5	0.0	0.056302055811378676	0.0
34-35	0.0	5.718847720810429E-5	0.0	0.09613383018682331	0.0
36-37	0.0	5.718847720810429E-5	0.0	0.16333029090634588	0.0
38-39	0.0	5.718847720810429E-5	0.0	0.2608080503075596	0.0
40-41	0.0	5.718847720810429E-5	0.0	0.37003804177503885	0.0
42-43	0.0	5.718847720810429E-5	0.0	0.4688883246292471	0.0
44-45	0.0	5.718847720810429E-5	0.0	0.5817211901608368	0.0
46-47	0.0	5.718847720810429E-5	0.0	0.7143412688064308	0.0
48-49	0.0	5.718847720810429E-5	0.0	0.8530233260360837	0.0
50-51	0.0	5.718847720810429E-5	0.0	0.9879881322472097	0.0
52-53	0.0	5.718847720810429E-5	0.0	1.1265844067610504	0.0
54-55	0.0	5.718847720810429E-5	0.0	1.2859686927400373	0.0
56-57	0.0	5.718847720810429E-5	0.0	1.4721743745296247	0.0
58-59	0.0	5.718847720810429E-5	0.0	1.6719623196561373	0.0
60-61	0.0	5.718847720810429E-5	0.0	1.8779838087983327	0.0
62-63	0.0	5.718847720810429E-5	0.0	2.0795445967182964	0.0
64-65	0.0	5.718847720810429E-5	0.0	2.3064398800414505	0.0
66-67	0.0	5.718847720810429E-5	0.0	2.567333713064822	0.0
68-69	0.0	5.718847720810429E-5	0.0	2.858022742713616	5.718847720810429E-5
70-71	0.0	5.718847720810429E-5	0.0	3.18808603892019	5.718847720810429E-5
72-73	0.0	5.718847720810429E-5	0.0	3.508713236387427	5.718847720810429E-5
74-75	0.0	5.718847720810429E-5	0.0	3.8409782889665127	5.718847720810429E-5
76-77	0.0	5.718847720810429E-5	0.0	4.215391249247972	5.718847720810429E-5
78-79	0.0	5.718847720810429E-5	0.0	4.633667771548046	5.718847720810429E-5
80-81	0.0	5.718847720810429E-5	0.0	5.081510736564711	5.718847720810429E-5
82-83	0.0	5.718847720810429E-5	0.0	5.549226697411192	5.718847720810429E-5
84-85	0.0	5.718847720810429E-5	0.0	6.025292175930057	5.718847720810429E-5
86-87	0.0	5.718847720810429E-5	0.0	6.5483093942367745	5.718847720810429E-5
88-89	0.0	5.718847720810429E-5	0.0	7.129658859295758	5.718847720810429E-5
90-91	0.0	5.718847720810429E-5	0.0	7.724304645305626	5.718847720810429E-5
92-93	0.0	5.718847720810429E-5	0.0	8.336793236204423	1.1437695441620859E-4
94-95	0.0	5.718847720810429E-5	0.0	8.97347255296225	1.1437695441620859E-4
96-97	0.0	5.718847720810429E-5	0.0	9.640518951117578	1.1437695441620859E-4
98-99	0.0	5.718847720810429E-5	0.0	10.359235138430428	1.1437695441620859E-4
100-101	0.0	5.718847720810429E-5	0.0	11.147664079459958	1.1437695441620859E-4
102-103	0.0	5.718847720810429E-5	0.0	11.936150208966696	1.1437695441620859E-4
104-105	0.0	5.718847720810429E-5	0.0	12.71359896237227	1.1437695441620859E-4
106-107	0.0	5.718847720810429E-5	0.0	13.52187230499301	1.1437695441620859E-4
108-109	0.0	5.718847720810429E-5	0.0	14.36385825492793	1.1437695441620859E-4
110-111	0.0	5.718847720810429E-5	0.0	15.254225656580907	1.1437695441620859E-4
112-113	0.0	5.718847720810429E-5	0.0	16.168840972570116	1.1437695441620859E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9550	0.0	30.648184	1
GGTATCA	7220	0.0	26.998352	1
TATCAAC	11595	0.0	25.02854	2
TCAACGC	11820	0.0	24.402266	4
ATCAACG	12000	0.0	24.334198	3
CAACGCA	11870	0.0	24.273052	5
GTATAGA	545	0.0	24.06317	1
AACGCAG	12125	0.0	23.84643	6
GAGTACT	6035	0.0	23.11815	12-13
AGTACTT	6420	0.0	22.473164	12-13
CATGGGG	4795	0.0	22.12247	4
GTACATG	11145	0.0	21.822613	1
GTACTTT	6375	0.0	21.792166	14-15
ACGCAGA	13310	0.0	21.589268	7
TACATGG	10800	0.0	21.463633	2
CGCAGAG	13450	0.0	21.18701	8
ACATGGG	11090	0.0	20.366196	3
GCAGAGT	14005	0.0	19.583336	9
GTATAGG	455	1.05501385E-10	18.341856	1
AGAGTAC	13040	0.0	18.067793	10-11
>>END_MODULE