FastQCFastQC Report
Mon 15 Aug 2016
SRR2921145_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921145_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901046
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA39710.13688166268304605No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38620.13312439720018227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG11850.027.1320171
GTATCAA113750.025.1244471
CATGGGG75900.024.7749234
GTACATG161800.023.882121
GTGTAGC18650.023.3050881
TACATGG162550.023.0652452
TCAACGC121800.022.3761444
AACGCAG123150.022.0802466
CAACGCA123750.022.023175
ATCAACG125100.021.930483
ACATGGG166100.021.9273573
TATCAAC130250.021.3375052
ACGCAGA134950.019.8850087
CGCAGAG136250.019.6076018
CGTATAG2158.524062E-519.3850211
TATACGA1959.905627E-418.3098034
GCAGAGT146600.017.5736149
GGTATCA87750.016.8950271
ATGGGGC37850.016.8219935
TAGCCCT26000.016.7076974
Produced by FastQC (version 0.11.5)