FastQCFastQC Report
Mon 15 Aug 2016
SRR2921145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901046
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42050.14494771885726734No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38990.1343997992448241No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38390.1323315797129725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35650.12288671051751679No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA124750.029.1680341
GGTATCA93000.027.3240171
TCAACGC144750.024.6777784
ATCAACG145550.024.6240833
CAACGCA144450.024.6018165
AACGCAG147200.024.2897136
TATCAAC152500.023.813062
CATGGGG71900.023.5552234
GTACATG152850.022.7520751
TACATGG156300.021.975122
GAGTACT69950.021.8579112-13
ACGCAGA166300.021.4642037
CGCAGAG168750.021.1522088
ACATGGG158550.020.799683
GTACTTT74200.020.5264714-15
AGTACTT78800.020.38453512-13
GCAGAGT178450.019.6030679
AGAGTAC166700.017.82637810-11
GTGTAGC19600.017.347491
TACCGCG3456.0192724E-717.243658
Produced by FastQC (version 0.11.5)