FastQCFastQC Report
Mon 15 Aug 2016
SRR2921144_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921144_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2924371
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41500.047.7727131
GTATCAA68600.044.001511
TCAACGC81100.036.3939484
ATCAACG83200.035.3341183
CAACGCA83050.035.323265
TATCAAC86600.034.221742
AACGCAG87100.033.4718256
ACGCAGA100300.028.7701637
GTACATG132650.028.5901181
TACATGG135900.027.1495632
CGCAGAG106650.027.0027668
ACATGGG137950.025.7107753
CATGGGG99300.025.4088464
GCAGAGT114900.023.3032369
AGAGTAC101500.020.95955510-11
CAGAGTA108700.020.41979210-11
ATGGGGA51000.019.6016465
ATGGGGG47950.017.497825
GTATAGA9800.017.0104471
GTATAGT7400.016.8955121
Produced by FastQC (version 0.11.5)