FastQCFastQC Report
Mon 15 Aug 2016
SRR2921144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2924371
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47100.055.0737041
GTATCAA79950.048.9284321
TCAACGC104150.037.0302964
ATCAACG106000.036.3827633
TATCAAC107500.036.2619932
CAACGCA107400.035.8599625
AACGCAG113050.034.0473636
ACGCAGA126450.029.7806847
CGCAGAG131750.028.3573888
GCAGAGT143350.025.275039
GTACATG127500.025.0218561
GTGGTAT27300.024.2457851
TACATGG128750.024.0734622
AGAGTAC130300.023.26332910-11
ACATGGG128150.023.1637063
GTATAGG8250.023.1298031
CATGGGG96200.021.7492474
CAGAGTA137550.021.69114310-11
GAGTACT63300.021.05311812-13
TGGTATC29850.020.7668862
Produced by FastQC (version 0.11.5)