##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921144_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2924371
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.67623704379506	31.0	31.0	34.0	27.0	34.0
2	30.800635418693457	31.0	31.0	34.0	27.0	34.0
3	30.876089935237356	31.0	31.0	34.0	27.0	34.0
4	34.370269709281075	37.0	35.0	37.0	30.0	37.0
5	34.00369002428214	37.0	35.0	37.0	30.0	37.0
6	33.86549449437161	37.0	35.0	37.0	29.0	37.0
7	33.69035871303607	37.0	35.0	37.0	28.0	37.0
8	33.690125158538365	37.0	35.0	37.0	28.0	37.0
9	35.115673763691404	39.0	35.0	39.0	28.0	39.0
10-11	35.06707322702899	39.0	35.0	39.0	28.0	39.0
12-13	34.94330815071002	39.0	35.0	39.0	27.0	39.0
14-15	36.04466943489728	40.0	36.0	41.0	27.0	41.0
16-17	35.986834091844024	40.0	36.0	41.0	27.0	41.0
18-19	36.0236652599824	40.0	36.0	41.0	27.0	41.0
20-21	36.03110002116695	40.0	36.0	41.0	27.0	41.0
22-23	35.99080862175148	40.0	36.0	41.0	27.0	41.0
24-25	35.88463262698201	40.0	36.0	41.0	27.0	41.0
26-27	35.78052733391215	40.0	35.0	41.0	27.0	41.0
28-29	35.68488745101084	39.0	35.0	41.0	27.0	41.0
30-31	35.59548207118728	39.0	35.0	41.0	26.5	41.0
32-33	35.4578208442089	39.0	35.0	41.0	26.0	41.0
34-35	35.33287619799266	39.0	35.0	41.0	25.0	41.0
36-37	35.193084769340146	39.0	35.0	41.0	25.0	41.0
38-39	35.020731295721376	39.0	34.0	41.0	24.0	41.0
40-41	34.86876477027026	39.0	34.0	41.0	24.0	41.0
42-43	34.72449460071927	39.0	34.0	41.0	23.5	41.0
44-45	34.54086776267444	39.0	34.0	41.0	23.0	41.0
46-47	34.30939918361931	38.5	33.0	41.0	22.0	41.0
48-49	34.093976790222584	38.0	33.0	41.0	20.5	41.0
50-51	33.87516546293203	38.0	33.0	40.0	19.0	41.0
52-53	33.68754939096305	38.0	33.0	40.0	18.0	41.0
54-55	33.46805740448117	38.0	33.0	40.0	17.0	41.0
56-57	33.18722504771111	37.5	32.0	40.0	14.5	41.0
58-59	32.94986990364765	37.0	32.0	40.0	13.0	41.0
60-61	32.6682748187559	36.5	32.0	40.0	11.5	41.0
62-63	32.36828825754324	36.0	31.5	39.5	8.5	41.0
64-65	32.06738337919505	36.0	31.0	39.0	8.0	41.0
66-67	31.731463962677786	35.0	31.0	39.0	7.0	41.0
68-69	31.371593070783426	35.0	31.0	38.5	6.5	40.0
70-71	31.01458108427419	35.0	30.5	37.5	2.0	40.0
72-73	30.824691702933727	35.0	30.5	37.0	2.0	39.0
74-75	30.626159779316644	35.0	30.0	37.0	2.0	39.0
76-77	30.39638421390446	35.0	30.0	36.0	2.0	39.0
78-79	30.082652303691972	35.0	30.0	36.0	2.0	37.5
80-81	29.797140650074837	35.0	30.0	35.0	2.0	37.0
82-83	29.59225265877688	34.0	30.0	35.0	2.0	37.0
84-85	29.444196033950547	34.0	30.0	35.0	2.0	36.0
86-87	29.245290183769434	34.0	30.0	35.0	2.0	36.0
88-89	29.11177018921334	34.0	29.0	35.0	2.0	36.0
90-91	28.961488299535183	34.0	29.0	35.0	2.0	35.0
92-93	28.85108045456613	34.0	29.0	35.0	2.0	35.0
94-95	28.758495074667337	34.0	29.0	35.0	2.0	35.0
96-97	28.69273751517848	34.0	29.0	35.0	2.0	35.0
98-99	28.65167005144012	34.0	29.0	35.0	2.0	35.0
100-101	28.51405191065019	34.0	29.0	35.0	2.0	35.0
102-103	27.14522319500501	32.0	26.0	34.0	2.0	35.0
104-105	28.237289317942217	34.0	29.0	35.0	2.0	35.0
106-107	28.525684155669715	34.0	29.0	35.0	2.0	35.0
108-109	28.734274652566313	34.0	29.5	35.0	2.0	35.0
110-111	28.71453245843294	34.0	30.0	35.0	2.0	35.0
112-113	28.64224477674002	34.0	29.5	35.0	2.0	35.0
114-115	28.530694292892385	34.0	29.0	35.0	2.0	35.0
116-117	28.4372256461304	34.0	29.0	35.0	2.0	35.0
118-119	28.299835930530016	34.0	29.0	35.0	2.0	35.0
120-121	28.13114033752899	34.0	29.0	35.0	2.0	35.0
122-123	27.953451699527864	34.0	29.0	35.0	2.0	35.0
124-125	27.162042025447526	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68408.0
3	23826.0
4	12257.0
5	10068.0
6	10996.0
7	13020.0
8	14911.0
9	16387.0
10	17285.0
11	18503.0
12	19798.0
13	20991.0
14	23173.0
15	19620.0
16	14753.0
17	13223.0
18	12298.0
19	11983.0
20	11722.0
21	12154.0
22	13603.0
23	16744.0
24	22901.0
25	30631.0
26	37774.0
27	44786.0
28	53127.0
29	62374.0
30	75180.0
31	90316.0
32	111272.0
33	139970.0
34	185610.0
35	269634.0
36	468897.0
37	708514.0
38	227454.0
39	208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.852998407319625	19.164215818799928	14.077186479122336	24.90559929475812
2	16.61790970975277	20.378652625574954	37.437238597454694	25.566199067217575
3	19.05839556085816	24.201420555480897	29.33028855311045	27.409895330550494
4	13.91917937839462	17.028679412310833	34.896493885831866	34.15564732346269
5	14.75609651771767	36.65801499812652	33.48359975567733	15.102288728478474
6	33.09801064953986	35.147653783938146	16.936632843946963	14.81770272257504
7	28.703797120293956	31.63702612091263	20.6966602701319	18.962516488661514
8	26.85320429800103	33.8496479377796	19.633064608521654	19.664083155697718
9	26.58334003115771	15.587575906301144	19.191165028122313	38.637919034418836
10-11	25.639757997947576	25.504149566694295	27.046870754220574	21.80922168113755
12-13	26.374267755773516	23.059218261151358	27.000524146210285	23.565989836864837
14-15	23.750047458434107	24.382638456347305	24.632859843529115	27.23445424168947
16-17	23.64392378937892	27.270399804075957	25.436433799359826	23.649242607185293
18-19	23.67173337528988	26.163045634581312	27.182179186362298	22.98304180376651
20-21	24.704165691839776	25.201258997481187	26.84129407756749	23.253281233111554
22-23	24.37809983786779	25.03362362067904	26.697360049802636	23.890916491650533
24-25	23.90388003269261	25.41585338469245	26.81739768124637	23.86286890136857
26-27	23.650981230492967	25.59377485142588	26.936525717217496	23.818718200863653
28-29	23.672862367067328	25.63204746455029	27.037397385119476	23.657692783262902
30-31	23.989700415205434	25.38969761045665	26.77869813822828	23.841903836109633
32-33	23.82962305882039	25.398710643052112	26.61512932817618	24.15653696995132
34-35	23.97768750640252	25.566093930077553	26.663197263287188	23.793021300232738
36-37	24.01613289957453	25.405537231439006	26.66127033500924	23.917059533977223
38-39	23.86887231007625	25.57323468141656	26.714761186422702	23.843131822084484
40-41	23.966114443536302	25.482782640878128	26.78498529098135	23.766117624604227
42-43	24.091711657230732	25.318599550687242	26.583437543610977	24.00625124847105
44-45	23.98162727908158	25.43676428863512	26.48137630198112	24.100232130302178
46-47	24.106467454928087	25.48461658837926	26.32288617583754	24.086029780855114
48-49	24.033498906770667	25.67117953331036	26.18895209885495	24.10636946106402
50-51	23.993150047682178	25.886139467300424	26.14050714138143	23.980203343635967
52-53	24.21249486933917	25.704491038445752	26.012484608017512	24.070529484197564
54-55	24.047383801920205	25.678534394782126	26.02868114582972	24.245400657467954
56-57	23.97958319829592	25.85891063074372	25.926345250545353	24.235160920415005
58-59	23.909301525773042	25.70321399626599	26.140712745695428	24.24677173226554
60-61	23.893427097968022	25.892737886858214	26.174784279487934	24.03905073568583
62-63	23.74700617543658	25.824896580079205	26.206235090736573	24.221862153747644
64-65	23.77675888051742	25.73653410284249	26.114960481244243	24.371746535395847
66-67	23.752207158548043	25.825431118523984	26.0465232421235	24.375838480804475
68-69	23.803845017198118	25.65647344211336	26.155690838890454	24.383990701798073
70-71	23.735202848674515	25.569090944231476	26.24024462559664	24.455461581497367
72-73	23.777505056508893	25.569335106496705	26.172849219822368	24.480310617172034
74-75	23.70932445485466	25.687930458909904	26.08577573586754	24.5169693503679
76-77	23.753678777918953	25.485325074858107	26.111995594871857	24.649000552351083
78-79	23.658638609509854	25.634613950733065	26.095717617057023	24.611029822700058
80-81	23.63636985522415	25.674741312893186	26.05806254665603	24.630826285226636
82-83	23.645286667655142	25.641616306513974	26.118308350353775	24.594788675477105
84-85	23.614442131896624	25.58384707292836	25.984356186857738	24.81735460831727
86-87	23.55012928685402	25.51290226543127	26.07603240097342	24.860936046741287
88-89	23.50860146486284	25.4502603807289	26.03306244434857	25.00807571005969
90-91	23.348555199557847	25.498364493013355	26.12029834492986	25.03278196249894
92-93	23.348739867283886	25.485098894077986	26.186610081985194	24.97955115665293
94-95	23.304446086849158	25.372832900748556	26.23599600122713	25.086725011175155
96-97	23.417072330697614	25.193711486428484	26.23499088688138	25.15422529599252
98-99	23.210484221665013	25.242548604200866	26.14953973265488	25.39742744147924
100-101	23.08522346783334	25.25560336896424	26.265533372596416	25.393639790606
102-103	23.017658076317403	25.185335159396015	26.322970615701074	25.474036148585505
104-105	22.941766742057386	25.306553383365042	26.151013773141774	25.6006661014358
106-107	22.917604328406192	25.320355410849743	26.12334726430775	25.638692996436312
108-109	22.819209804698108	25.255028580848197	26.17356923049563	25.75219238395806
110-111	22.71130011901342	25.364957387723834	26.169529144608145	25.754213348654602
112-113	22.661758004433867	25.35217457099791	26.209263931506722	25.7768034930615
114-115	22.56477828603946	25.46160769979974	26.127361729719084	25.84625228444172
116-117	22.48683504742324	25.43810646969979	26.12266519478419	25.952393288092786
118-119	22.480336239573944	25.30667532537259	26.05403586489341	26.158952570160054
120-121	22.31080772159156	25.422157522834148	26.094576348099125	26.172458407475176
122-123	22.23074122818342	25.402210978426265	26.06102803845385	26.306019754936464
124-125	22.20850778609312	25.397071417598248	26.081915043943795	26.31250575236484
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	37.5
2	89.0
3	131.5
4	122.0
5	102.0
6	140.0
7	333.5
8	546.0
9	641.5
10	598.0
11	513.0
12	520.5
13	564.0
14	610.5
15	679.5
16	797.0
17	873.0
18	988.5
19	1192.0
20	1381.0
21	1585.0
22	1900.5
23	2322.5
24	2902.5
25	3676.0
26	4866.0
27	6595.5
28	8735.0
29	11571.5
30	15232.5
31	20036.0
32	26293.0
33	31574.0
34	36444.5
35	42857.5
36	50062.5
37	57503.5
38	64384.5
39	70338.5
40	75809.5
41	81520.5
42	87482.5
43	94350.0
44	101247.5
45	107658.0
46	113916.5
47	118412.5
48	121895.5
49	125407.0
50	126765.0
51	126100.5
52	124426.0
53	122985.0
54	120663.0
55	114374.0
56	105742.0
57	96849.5
58	89082.5
59	79857.0
60	68151.0
61	57364.5
62	47255.5
63	36144.0
64	26508.5
65	20166.0
66	15378.0
67	11703.0
68	8935.0
69	6796.5
70	5279.0
71	4101.0
72	3123.5
73	2641.0
74	2095.0
75	1494.5
76	1095.0
77	696.0
78	428.5
79	256.0
80	166.5
81	109.0
82	64.0
83	31.5
84	18.0
85	13.0
86	9.0
87	6.5
88	6.0
89	6.0
90	3.5
91	4.5
92	4.0
93	2.0
94	5.0
95	6.0
96	4.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07704220839284756
2	6.83907753154439E-5
3	7.52298528469883E-4
4	0.1850996333912489
5	0.06859594764139024
6	0.010292811684974307
7	0.013404591961827006
8	0.010737351724524693
9	0.018328727784538965
10-11	0.01749094078692478
12-13	0.019440077883414928
14-15	0.025988494619868685
16-17	0.026980160861942618
18-19	0.024312920624640307
20-21	0.02650142543473451
22-23	0.02841636714356694
24-25	0.02648432774090565
26-27	0.025509759192660575
28-29	0.02590300615072438
30-31	0.025920103844553236
32-33	0.02275703048621396
34-35	0.024535190644415502
36-37	0.026672402373023124
38-39	0.032861767539070795
40-41	0.028194097123791752
42-43	0.02718533318788895
44-45	0.027270821657033255
46-47	0.02901478642757708
48-49	0.023714501340630172
50-51	0.02896349334609049
52-53	0.026364643884103624
54-55	0.030177429607939624
56-57	0.027305017044690977
58-59	0.024124845992522837
60-61	0.02525329378522766
62-63	0.029168665672036822
64-65	0.03245142288717813
66-67	0.03176751513402369
68-69	0.02718533318788895
70-71	0.035785473183806024
72-73	0.03961535660147088
74-75	0.025543954580318298
76-77	0.017302866154807306
78-79	0.021372117286076218
80-81	0.024398409093784615
82-83	0.02326996130107979
84-85	0.01598634372998501
86-87	0.015268240589172852
88-89	0.016396688381877675
90-91	0.01733706154246503
92-93	0.020910479552696974
94-95	0.016208613749760205
96-97	0.01928619863895518
98-99	0.016157320668273623
100-101	0.017268670767149587
102-103	0.014071402021152582
104-105	0.0168241307275992
106-107	0.014738212080478161
108-109	0.01316522424822295
110-111	0.011318673314705966
112-113	0.009865369339252784
114-115	0.008309479200826433
116-117	0.007676864529158578
118-119	0.0050096242918562656
120-121	0.002650142543473451
122-123	0.006514221348796033
124-125	0.05676434351181844
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2924371.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.29754695045951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.10821507949012	45.72328003942195
2	12.680065624815143	15.037935734447675
3	4.37135796197727	7.776324119628366
4	1.9477427091612782	4.619854589756244
5	1.0646562610265393	3.1565752312160944
6	0.6263079344322552	2.2283114488465183
7	0.42652904652607637	1.7704488313480322
8	0.29015660296629836	1.3764459829903934
9	0.22312344933442863	1.190760589139108
>10	1.1876773385225725	12.303916788604978
>50	0.053387467333852454	2.1465203534494974
>100	0.019624165483842194	1.9738884168121684
>500	8.094512513199224E-4	0.3045160302133044
>1k	3.4690767898700484E-4	0.39122184412560407
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	3.419538765772195E-5	0.0
12-13	0.0	0.0	0.0	3.419538765772195E-5	0.0
14-15	0.0	0.0	0.0	3.419538765772195E-5	0.0
16-17	0.0	0.0	0.0	1.0258616297316586E-4	5.129308148658293E-5
18-19	0.0	0.0	0.0	1.367815506308878E-4	1.0258616297316586E-4
20-21	0.0	0.0	0.0	1.5387924445974877E-4	1.7097693828860974E-4
22-23	0.0	0.0	0.0	3.0775848891949754E-4	1.8807463211747071E-4
24-25	0.0	0.0	0.0	8.890800791007707E-4	2.051723259463317E-4
26-27	0.0	0.0	0.0	0.0015729878322552097	2.051723259463317E-4
28-29	0.0	3.419538765772195E-5	0.0	0.003983762662124607	2.051723259463317E-4
30-31	0.0	3.419538765772195E-5	0.0	0.01297714961610548	2.2227001977519268E-4
32-33	0.0	3.419538765772195E-5	0.0	0.03361406606754068	2.3936771360405366E-4
34-35	0.0	5.129308148658293E-5	0.0	0.06298790406552383	3.419538765772195E-4
36-37	0.0	6.83907753154439E-5	0.0	0.10513371935366614	3.419538765772195E-4
38-39	0.0	6.83907753154439E-5	0.0	0.16865165192788467	3.9324695806380243E-4
40-41	0.0	6.83907753154439E-5	0.0	0.23757245575202326	4.103446518926634E-4
42-43	0.0	6.83907753154439E-5	0.0	0.30871595977391375	4.274423457215244E-4
44-45	0.0	6.83907753154439E-5	0.0	0.39801721464205464	4.4454003955038537E-4
46-47	0.0	6.83907753154439E-5	0.0	0.5000904468003546	4.4454003955038537E-4
48-49	0.0	6.83907753154439E-5	0.0	0.6048651145836148	4.787354272081073E-4
50-51	0.0	6.83907753154439E-5	0.0	0.7073486913938074	4.787354272081073E-4
52-53	0.0	6.83907753154439E-5	0.0	0.8136963470093227	4.958331210369683E-4
54-55	0.0	6.83907753154439E-5	0.0	0.9418777576442934	5.129308148658293E-4
56-57	0.0	6.83907753154439E-5	0.0	1.087430425209387	5.129308148658293E-4
58-59	0.0	1.0258616297316586E-4	0.0	1.2394118256541322	5.129308148658293E-4
60-61	0.0	1.0258616297316586E-4	0.0	1.3930517024002769	5.300285086946902E-4
62-63	0.0	1.0258616297316586E-4	0.0	1.554385541369409	5.471262025235512E-4
64-65	0.0	1.0258616297316586E-4	0.0	1.739365490903856	5.642238963524122E-4
66-67	0.0	1.0258616297316586E-4	0.0	1.9594641035627833	5.813215901812732E-4
68-69	0.0	1.0258616297316586E-4	0.0	2.194642882178766	5.813215901812732E-4
70-71	0.0	1.0258616297316586E-4	0.0	2.435566485921246	5.813215901812732E-4
72-73	0.0	1.367815506308878E-4	0.0	2.680696122345626	7.52298528469883E-4
74-75	0.0	1.367815506308878E-4	0.0	2.955165401380331	7.864939161276049E-4
76-77	0.0	1.367815506308878E-4	0.0	3.2717975933970074	7.864939161276049E-4
78-79	0.0	1.367815506308878E-4	0.0	3.608160524092189	7.864939161276049E-4
80-81	0.0	1.367815506308878E-4	0.0	3.9514138254004023	8.206893037853269E-4
82-83	0.0	1.367815506308878E-4	0.0	4.309114677994003	8.548846914430487E-4
84-85	0.0	1.367815506308878E-4	0.0	4.694308622264412	8.548846914430487E-4
86-87	0.0	1.367815506308878E-4	0.0	5.125974098361665	8.719823852719097E-4
88-89	0.0	1.367815506308878E-4	0.0	5.598058522670345	8.890800791007707E-4
90-91	0.0	1.367815506308878E-4	0.0	6.07833274232305	8.890800791007707E-4
92-93	0.0	1.367815506308878E-4	0.0	6.564215005551621	9.403731605873536E-4
94-95	0.0	1.367815506308878E-4	0.0	7.0842755587440855	0.0010087639359027977
96-97	0.0	1.367815506308878E-4	0.0	7.64890979974839	0.0010429593235605195
98-99	0.0	1.367815506308878E-4	0.0	8.249739858588395	0.0010600570173893804
100-101	0.0	1.367815506308878E-4	0.0	8.873703097178847	0.0010600570173893804
102-103	0.0	1.367815506308878E-4	0.0	9.501239753779531	0.0010771547112182413
104-105	0.0	1.7097693828860974E-4	0.0	10.15165312472323	0.0010942524050471024
106-107	0.0	1.8807463211747071E-4	0.0	10.84005757135466	0.0010942524050471024
108-109	0.0	2.051723259463317E-4	0.0	11.567667029935668	0.0011113500988759635
110-111	0.0	2.051723259463317E-4	0.0	12.318700329062215	0.0011284477927048244
112-113	0.0	2.051723259463317E-4	0.0	13.07530747637697	0.0011284477927048244
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4710	0.0	55.073704	1
GTATCAA	7995	0.0	48.928432	1
TCAACGC	10415	0.0	37.030296	4
ATCAACG	10600	0.0	36.382763	3
TATCAAC	10750	0.0	36.261993	2
CAACGCA	10740	0.0	35.859962	5
AACGCAG	11305	0.0	34.047363	6
ACGCAGA	12645	0.0	29.780684	7
CGCAGAG	13175	0.0	28.357388	8
GCAGAGT	14335	0.0	25.27503	9
GTACATG	12750	0.0	25.021856	1
GTGGTAT	2730	0.0	24.245785	1
TACATGG	12875	0.0	24.073462	2
AGAGTAC	13030	0.0	23.263329	10-11
ACATGGG	12815	0.0	23.163706	3
GTATAGG	825	0.0	23.129803	1
CATGGGG	9620	0.0	21.749247	4
CAGAGTA	13755	0.0	21.691143	10-11
GAGTACT	6330	0.0	21.053118	12-13
TGGTATC	2985	0.0	20.766886	2
>>END_MODULE