FastQCFastQC Report
Mon 15 Aug 2016
SRR2921143_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921143_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815745
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG80500.026.9805034
GTATCAA81100.026.5744651
GTACATG142600.025.2170471
TACATGG143050.024.502122
ACATGGG141400.023.9884223
GGTATCA62900.023.1895471
TCAACGC92700.022.7235744
CAACGCA94800.022.0946645
TACCGTA2454.3497676E-721.8574077
TATCAAC97700.021.8054332
ATCAACG96600.021.745723
AACGCAG96050.021.6816796
GTATATA9950.020.3438281
GTACCGT3852.3101165E-1020.0911526
TATACCG2701.1056509E-619.8349825
ACGCAGA106850.019.3788037
ATGGGGA42450.018.9238345
CGCAGAG110050.018.7612448
CCGTATG3503.4098775E-818.7002269
GTATAGG7050.018.578491
Produced by FastQC (version 0.11.5)