##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921143_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2815745
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.548989343850383	31.0	30.0	34.0	25.0	34.0
2	29.534545564317792	31.0	30.0	34.0	25.0	34.0
3	29.648663497582344	31.0	30.0	34.0	25.0	34.0
4	33.103764723012915	37.0	35.0	37.0	26.0	37.0
5	33.176235063899604	37.0	35.0	37.0	27.0	37.0
6	33.16202212913456	37.0	35.0	37.0	26.0	37.0
7	33.05724310972762	37.0	35.0	37.0	26.0	37.0
8	32.981567578029974	37.0	35.0	37.0	26.0	37.0
9	34.342925229379794	38.0	35.0	39.0	26.0	39.0
10-11	34.29435779873533	38.5	34.5	39.0	25.5	39.0
12-13	34.30600249667495	39.0	35.0	39.0	26.0	39.0
14-15	35.33708254831315	39.0	35.0	41.0	25.0	41.0
16-17	35.23670822464392	39.0	35.0	41.0	25.0	41.0
18-19	35.305506535570515	39.5	35.0	41.0	25.0	41.0
20-21	35.33620445033198	39.5	35.0	41.0	25.0	41.0
22-23	35.30331049153954	39.0	35.0	41.0	25.0	41.0
24-25	35.2082850187073	39.0	35.0	41.0	25.0	41.0
26-27	35.146819935754124	39.0	34.5	41.0	24.5	41.0
28-29	34.997640766475655	39.0	34.0	41.0	24.0	41.0
30-31	34.896179874242875	39.0	34.0	41.0	24.0	41.0
32-33	34.8038137331328	39.0	34.0	41.0	24.0	41.0
34-35	34.70499601348844	39.0	34.0	41.0	23.0	41.0
36-37	34.56735464326493	39.0	34.0	41.0	22.5	41.0
38-39	34.40684294920172	39.0	34.0	41.0	21.0	41.0
40-41	34.29901358255098	39.0	33.0	41.0	20.5	41.0
42-43	34.209906081694186	39.0	33.0	41.0	19.5	41.0
44-45	34.04693908716877	38.0	33.0	41.0	18.5	41.0
46-47	33.819517392377506	38.0	33.0	40.0	18.0	41.0
48-49	33.684707244441526	38.0	33.0	40.0	16.0	41.0
50-51	33.51521000658796	38.0	33.0	40.0	14.5	41.0
52-53	33.30751595048557	38.0	32.5	40.0	13.0	41.0
54-55	33.055638383447366	38.0	32.0	40.0	9.5	41.0
56-57	32.78292938458561	37.0	32.0	40.0	8.0	41.0
58-59	32.550286158725314	37.0	31.0	40.0	8.0	41.0
60-61	32.251463467039805	36.5	31.0	40.0	7.0	41.0
62-63	31.96975152224367	36.0	31.0	39.5	4.0	41.0
64-65	31.647283045872406	35.5	31.0	39.0	2.0	41.0
66-67	31.358462857964767	35.0	30.5	39.0	2.0	40.5
68-69	30.992521162250135	35.0	30.0	38.5	2.0	40.0
70-71	30.66905774493074	35.0	30.0	37.5	2.0	40.0
72-73	30.3035590935969	35.0	29.5	37.0	2.0	39.0
74-75	30.23210713328089	35.0	30.0	37.0	2.0	39.0
76-77	29.98362103102376	35.0	30.0	36.0	2.0	39.0
78-79	29.720812431523452	34.5	29.5	36.0	2.0	38.0
80-81	29.377446998929237	34.0	29.0	35.0	2.0	37.0
82-83	29.12967118826456	34.0	29.0	35.0	2.0	37.0
84-85	28.945842396950006	34.0	29.0	35.0	2.0	36.0
86-87	28.740353441096406	34.0	29.0	35.0	2.0	36.0
88-89	28.582345880042404	34.0	29.0	35.0	2.0	36.0
90-91	28.426642860060127	34.0	29.0	35.0	2.0	35.0
92-93	28.612848287043036	34.0	29.0	35.0	2.0	35.0
94-95	28.59692585798785	34.0	29.0	35.0	2.0	35.0
96-97	28.536780319240556	34.0	29.0	35.0	2.0	35.0
98-99	28.425725518468468	34.0	29.0	35.0	2.0	35.0
100-101	28.319048422353585	34.0	29.0	35.0	2.0	35.0
102-103	26.989029191208722	32.5	26.0	34.0	2.0	35.0
104-105	28.01095482723045	34.0	28.5	35.0	2.0	35.0
106-107	28.177917922255034	34.0	29.0	35.0	2.0	35.0
108-109	28.11729702085949	34.0	29.0	35.0	2.0	35.0
110-111	28.08314939740637	34.0	29.0	35.0	2.0	35.0
112-113	27.99378121953515	34.0	29.0	35.0	2.0	35.0
114-115	27.852557848810882	34.0	29.0	35.0	2.0	35.0
116-117	27.679193783527985	34.0	28.5	35.0	2.0	35.0
118-119	27.55512981466717	34.0	28.0	35.0	2.0	35.0
120-121	27.363435964549346	34.0	27.0	35.0	2.0	35.0
122-123	27.197181385388237	34.0	27.0	35.0	2.0	35.0
124-125	26.119019477971193	33.0	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	116231.0
3	16675.0
4	11276.0
5	10595.0
6	12420.0
7	13495.0
8	13740.0
9	15007.0
10	16031.0
11	17193.0
12	17371.0
13	17547.0
14	17324.0
15	15832.0
16	13325.0
17	12021.0
18	11183.0
19	10831.0
20	10962.0
21	12020.0
22	14381.0
23	18967.0
24	25189.0
25	32736.0
26	38515.0
27	44807.0
28	52633.0
29	61978.0
30	72679.0
31	87649.0
32	109046.0
33	138312.0
34	184363.0
35	264517.0
36	447758.0
37	644832.0
38	196188.0
39	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7625388760689	18.534027022310802	13.032328043475093	24.671106058145202
2	16.199972011817547	20.085463406872165	38.48623713094136	25.228327450368926
3	19.46345057179414	23.954718251873597	28.841520985914194	27.74031019041807
4	13.248384068336405	16.15732746908	35.934903093829355	34.659385368754236
5	14.460834108813481	37.252029863681244	33.7651751401192	14.521960887386076
6	33.44327193180918	35.23250308736141	17.17201602124801	14.152208959581406
7	28.94139116468602	31.744306600220142	20.757827126081114	18.556475109012723
8	26.5514580030964	34.732123164066145	19.74946803957622	18.96695079326123
9	26.57689750503624	16.107269803295363	19.194151709480636	38.12168098218776
10-11	25.422125075964573	25.99615266722241	27.296137003690696	21.285585253122317
12-13	25.84980882919648	23.508290579621352	27.1920889206175	23.449811670564667
14-15	23.403882385136768	25.024351703056443	24.483846008087696	27.087919903719094
16-17	23.493364163820708	27.73264497521662	25.45511375846018	23.31887710250249
18-19	23.507516690684916	27.105476959795848	26.69378375762613	22.693222591893107
20-21	24.54112603316793	26.2910381228755	26.258147241747977	22.909688602208593
22-23	24.104290636043814	26.025103071904116	26.035242893112965	23.8353633989391
24-25	23.798228932502713	26.22392406148996	26.223639934913706	23.75420707109362
26-27	23.594074218767343	26.35820166657048	26.181138541995004	23.86658557266718
28-29	23.611798538102516	26.48453595614627	26.311277715591164	23.592387790160046
30-31	23.97781336955099	26.381341323664714	26.113100742141153	23.52774456464314
32-33	23.666916340723045	26.37009082382139	26.01351507331645	23.94947776213911
34-35	23.732037516324684	26.441234144586385	26.185011584001682	23.64171675508725
36-37	24.0035090772512	26.191958601412512	26.06178888354161	23.74274343779468
38-39	23.817480879566574	26.347202565609678	26.14084216919031	23.694474385633438
40-41	23.858479435795772	26.302172305607307	26.25507952145776	23.584268737139162
42-43	23.826203167723413	26.212383861565435	26.127822686474083	23.833590284237065
44-45	23.887399516175346	26.246413178725852	26.082083979785413	23.784103325313392
46-47	23.81496618450996	26.260318452505153	26.077376518189865	23.847338844795026
48-49	23.677337818225148	26.344264458031475	26.16807227963592	23.810325444107463
50-51	23.61157701211966	26.499206305938827	26.080241679353282	23.808975002588227
52-53	23.802549104254787	26.368949748660732	25.924642081050454	23.903859066034027
54-55	23.80899445061043	26.27446836847947	25.941877913429522	23.97465926748058
56-57	23.771559734197016	26.33369848895184	25.92733338080534	23.967408396045805
58-59	23.724263974029704	26.112640345834937	26.173620319223232	23.98947536091212
60-61	23.67719368780068	26.228625525221926	26.203906065342125	23.89027472163526
62-63	23.53425752673288	26.215296799477073	26.23136751488672	24.01907815890333
64-65	23.605050221281918	26.190662226229993	26.123519385830356	24.08076816665773
66-67	23.68493063843608	26.261717044311812	25.966037418894977	24.087314898357132
68-69	23.869136988502827	26.040450084659454	26.07065579781883	24.01975712901888
70-71	23.63917287054012	25.983746928842915	26.228622651306203	24.148457549310763
72-73	23.759679708376368	26.025115034215485	26.13231811524305	24.082887142165102
74-75	23.626144239782974	26.123338966668612	26.109416927601316	24.1410998659471
76-77	23.70588880834834	25.930080544888174	26.237979441679983	24.126051205083503
78-79	23.716815322161487	26.025197297263226	26.191565729559045	24.066421651016242
80-81	23.638582654234657	26.06648620505714	26.159942920906815	24.134988219801386
82-83	23.741207251299702	25.953535895581396	26.288545103628934	24.016711749489968
84-85	23.728888501383917	25.9287585530525	26.137478515818874	24.20487442974471
86-87	23.619447683183704	25.905632588815937	26.300804158339957	24.174115569660398
88-89	23.794599224929538	25.791105299345396	26.14820452427493	24.26609095145013
90-91	23.59749684317482	25.951597892478475	26.240386799205673	24.210518465141032
92-93	23.67451911235714	25.860064506058784	26.378815674727228	24.086600706856846
94-95	23.54952787260773	25.808192420213356	26.43423289842269	24.20804680875622
96-97	23.668096138649904	25.676921574372496	26.420051674080387	24.234930612897212
98-99	23.495833380686662	25.780601851325702	26.321885903103727	24.40167886488391
100-101	23.381830244002227	25.791718045027334	26.51942031003171	24.307031400938733
102-103	23.371129339551594	25.678045364086294	26.60907974274826	24.34174555361385
104-105	23.296520526494586	25.796679016131847	26.424049368500924	24.482751088872643
106-107	23.169714944186982	25.88493811191301	26.468329292463277	24.477017651436732
108-109	23.166675930969166	25.853477567571844	26.505873893367227	24.473972608091767
110-111	23.1827814308448	25.974895920591536	26.475638501398585	24.366684147165078
112-113	23.14446112090942	26.06990216921111	26.452296747000194	24.33333996287928
114-115	23.115268858144024	26.154222904459267	26.386928562346434	24.343579675050275
116-117	23.031922629500283	26.072162910908254	26.487139869556348	24.408774590035122
118-119	23.119699500677182	25.994050886214097	26.38357915320575	24.50267045990297
120-121	23.030688002818554	26.106982172360837	26.491514504046698	24.370815320773907
122-123	23.067564930354614	26.04089847440902	26.423854430567435	24.46768216466893
124-125	23.02188358458351	26.21251174527865	26.330750956728146	24.43485371340969
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	694.0
1	518.0
2	279.5
3	210.5
4	183.0
5	150.5
6	147.5
7	159.0
8	177.0
9	202.0
10	220.5
11	241.5
12	276.0
13	308.0
14	352.5
15	425.5
16	493.0
17	572.0
18	684.0
19	825.5
20	959.0
21	1141.5
22	1506.0
23	1985.5
24	2569.0
25	3346.5
26	4593.0
27	6404.0
28	8665.0
29	11874.0
30	16008.5
31	21058.0
32	27082.0
33	32786.0
34	38935.0
35	45882.5
36	53783.0
37	61570.0
38	68076.5
39	73772.5
40	78656.0
41	83677.5
42	88560.0
43	94514.5
44	99718.5
45	103789.5
46	108310.5
47	112062.5
48	115923.0
49	120352.5
50	123918.0
51	123366.0
52	118101.5
53	113673.0
54	110608.0
55	105518.0
56	98589.0
57	89550.5
58	81171.0
59	73340.5
60	63352.5
61	52908.0
62	42888.5
63	32149.5
64	23097.5
65	17340.5
66	12962.5
67	9686.5
68	7237.0
69	5395.0
70	4148.5
71	3131.5
72	2360.5
73	1918.0
74	1453.0
75	1051.0
76	731.0
77	469.5
78	340.5
79	216.0
80	143.5
81	107.0
82	80.5
83	60.5
84	49.5
85	43.0
86	34.5
87	33.5
88	29.0
89	21.0
90	12.5
91	9.5
92	9.0
93	10.0
94	10.5
95	12.0
96	9.5
97	5.0
98	7.5
99	12.5
100	22.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0708871009271081
2	0.009624451077778704
3	0.020278824964618598
4	0.017615231492908626
5	0.0101216551924979
6	0.00831041163173512
7	0.008807615746454313
8	0.007671149198524724
9	0.005433730682288347
10-11	0.0102281989313663
12-13	0.007102915924559931
14-15	0.008292654341923719
16-17	0.012430102867979877
18-19	0.006303837883046938
20-21	0.013317967358549869
22-23	0.00403090478718776
24-25	0.0034981860928457657
26-27	0.0029299528188809713
28-29	0.010459043698914497
30-31	0.006232808723801338
32-33	0.002628078892087174
34-35	0.007955265835507121
36-37	0.006747770128331933
38-39	0.0014205831849119861
40-41	9.588936498155905E-4
42-43	0.0011364665479295887
44-45	0.0019710591690653805
46-47	0.0034626715132229655
48-49	0.0024682632837845758
50-51	0.00403090478718776
52-53	0.0045636234815297555
54-55	0.004261749554735958
56-57	0.0017934862709513824
58-59	0.0023617195449161764
60-61	0.0053094296536085476
62-63	0.0022906903856705772
64-65	0.003764545440016763
66-67	0.00101216551924979
68-69	0.0018645154301969817
70-71	0.001100951968306789
72-73	0.002166389356990779
74-75	0.002326204965293377
76-77	0.0017046998218943833
78-79	2.308447675481977E-4
80-81	1.0654373886839896E-4
82-83	5.327186943419948E-5
84-85	3.7290308603939637E-4
86-87	2.1308747773679792E-4
88-89	3.5514579622799646E-5
90-91	7.280488822673928E-4
92-93	0.0014383404747233858
94-95	0.0011542238377409888
96-97	7.102915924559931E-4
98-99	0.0011719811275523885
100-101	0.0011719811275523885
102-103	0.002113117487556579
104-105	0.003675758990959764
106-107	0.004918769277757752
108-109	0.009890810424949702
110-111	0.00804405228456412
112-113	0.0023794768347275767
114-115	0.0060729931154987395
116-117	0.0016514279524601837
118-119	0.001118709258118189
120-121	0.00415520581586756
122-123	5.859905637761942E-4
124-125	0.08454245679207457
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2815745.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.542626586269925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.16549374911084	35.46348349802566
2	14.245966497369297	14.400571300740964
3	6.067144286639274	9.199482243738895
4	3.163816758491187	6.396304360472121
5	1.794802888170702	4.535702608638529
6	1.1375372236704766	3.449647147437547
7	0.7492368828904228	2.6507879997614046
8	0.5014310529296083	2.0274913973585074
9	0.38112666977165593	1.7336828657103676
>10	1.6845040485101226	14.229937967079906
>50	0.07638005298589104	2.6089293690140893
>100	0.031147560677187398	2.6920858263168532
>500	0.0012004794658774932	0.4099157652572974
>1k	2.1184931748441282E-4	0.20197765044793564
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.130874777367979E-4
2	0.0	0.0	0.0	0.0	2.130874777367979E-4
3	0.0	0.0	0.0	0.0	2.130874777367979E-4
4	0.0	0.0	0.0	0.0	2.130874777367979E-4
5	0.0	0.0	0.0	0.0	2.130874777367979E-4
6	0.0	0.0	0.0	0.0	2.4860205735959754E-4
7	0.0	0.0	0.0	0.0	2.4860205735959754E-4
8	0.0	0.0	0.0	0.0	2.4860205735959754E-4
9	0.0	0.0	0.0	0.0	2.4860205735959754E-4
10-11	0.0	0.0	0.0	0.0	2.841166369823972E-4
12-13	0.0	0.0	0.0	3.551457962279965E-5	0.0011719811275523885
14-15	0.0	0.0	0.0	3.551457962279965E-5	0.0013673113154777866
16-17	0.0	0.0	0.0	7.10291592455993E-5	0.0015626415034031846
18-19	0.0	0.0	0.0	8.878644905699912E-5	0.0018645154301969815
20-21	0.0	0.0	0.0	2.3084476754819773E-4	0.002113117487556579
22-23	3.551457962279965E-5	0.0	0.0	4.261749554735958E-4	0.002201903936613578
24-25	3.551457962279965E-5	0.0	0.0	9.05621780381391E-4	0.002201903936613578
26-27	3.551457962279965E-5	0.0	0.0	0.002130874777367979	0.0023794768347275763
28-29	3.551457962279965E-5	0.0	0.0	0.005487002551722546	0.0024860205735959756
30-31	3.551457962279965E-5	0.0	0.0	0.01717129924762363	0.002557049732841575
32-33	3.551457962279965E-5	0.0	0.0	0.04148102899942999	0.002734622630955573
34-35	3.551457962279965E-5	0.0	0.0	0.07607222955203685	0.0030187392679379703
36-37	3.551457962279965E-5	0.0	0.0	0.13161703208209552	0.0030187392679379703
38-39	3.551457962279965E-5	0.0	0.0	0.2148632067179379	0.0031252830068063692
40-41	3.551457962279965E-5	0.0	0.0	0.30872824066099736	0.003160797586429169
42-43	3.551457962279965E-5	0.0	0.0	0.39811843757158405	0.0032495840354861676
44-45	3.551457962279965E-5	0.0	0.0	0.5055855555101758	0.003391642353977367
46-47	3.551457962279965E-5	0.0	0.0	0.6340062754262193	0.003515943382657165
48-49	3.551457962279965E-5	0.0	0.0	0.7635634618901925	0.0036935162807711633
50-51	3.551457962279965E-5	0.0	0.0	0.8953225522907792	0.003906603758507961
52-53	3.551457962279965E-5	0.0	0.0	1.0291947601789224	0.004172963105678959
54-55	3.551457962279965E-5	0.0	0.0	1.1787999268399658	0.004368293293604357
56-57	5.327186943419948E-5	0.0	0.0	1.3512232109086582	0.004421565163038556
58-59	7.10291592455993E-5	0.0	0.0	1.5327914992302216	0.004474837032472756
60-61	7.10291592455993E-5	0.0	0.0	1.7143065156823505	0.004474837032472756
62-63	7.10291592455993E-5	0.0	0.0	1.9015571367435617	0.004616895350963954
64-65	7.10291592455993E-5	0.0	0.0	2.1080744172501413	0.004723439089832354
66-67	7.10291592455993E-5	0.0	0.0	2.3501773065387668	0.004758953669455153
68-69	7.10291592455993E-5	0.0	0.0	2.6220946854207323	0.004758953669455153
70-71	7.10291592455993E-5	0.0	0.0	2.9051813995940683	0.004794468249077952
72-73	7.10291592455993E-5	0.0	0.0	3.1933822132330874	0.00502531301662615
74-75	7.10291592455993E-5	0.0	0.0	3.498647782380862	0.005256157784174348
76-77	7.10291592455993E-5	0.0	0.0	3.8460336429612765	0.0053271869434199474
78-79	7.10291592455993E-5	0.0	0.0	4.2259508584761765	0.005380458812854147
80-81	7.10291592455993E-5	0.0	0.0	4.622915072210019	0.005487002551722546
82-83	8.878644905699912E-5	0.0	0.0	5.037654333045073	0.005824391058139143
84-85	1.0654373886839895E-4	0.0	0.0	5.464699395719428	0.005842148347950543
86-87	1.0654373886839895E-4	0.0	0.0	5.935125517403032	0.00607299311549874
88-89	1.0654373886839895E-4	0.0	0.0	6.446659765000026	0.006286080593235538
90-91	1.0654373886839895E-4	0.0	0.0	6.979911178036364	0.006286080593235538
92-93	1.0654373886839895E-4	0.0	0.0	7.538750845690927	0.006534682650595136
94-95	1.0654373886839895E-4	0.0	0.0	8.102473768043627	0.006534682650595136
96-97	1.0654373886839895E-4	0.0	0.0	8.703486999000265	0.006783284707954734
98-99	1.0654373886839895E-4	0.0	0.0	9.358624449302049	0.006925343026445932
100-101	1.0654373886839895E-4	0.0	0.0	10.0518690435391	0.006925343026445932
102-103	1.0654373886839895E-4	0.0	0.0	10.75099130070372	0.007014129475502931
104-105	1.0654373886839895E-4	0.0	0.0	11.451463111894011	0.00713843050418273
106-107	1.0654373886839895E-4	0.0	0.0	12.174983885259497	0.0071739450838055295
108-109	1.0654373886839895E-4	0.0	0.0	12.939932415754978	0.0071739450838055295
110-111	1.0654373886839895E-4	0.0	0.0	13.74078618624911	0.0071739450838055295
112-113	1.0654373886839895E-4	0.0	0.0	14.554922409522169	0.0071739450838055295
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	8050	0.0	26.980503	4
GTATCAA	8110	0.0	26.574465	1
GTACATG	14260	0.0	25.217047	1
TACATGG	14305	0.0	24.50212	2
ACATGGG	14140	0.0	23.988422	3
GGTATCA	6290	0.0	23.189547	1
TCAACGC	9270	0.0	22.723574	4
CAACGCA	9480	0.0	22.094664	5
TACCGTA	245	4.3497676E-7	21.857407	7
TATCAAC	9770	0.0	21.805433	2
ATCAACG	9660	0.0	21.74572	3
AACGCAG	9605	0.0	21.681679	6
GTATATA	995	0.0	20.343828	1
GTACCGT	385	2.3101165E-10	20.091152	6
TATACCG	270	1.1056509E-6	19.834982	5
ACGCAGA	10685	0.0	19.378803	7
ATGGGGA	4245	0.0	18.923834	5
CGCAGAG	11005	0.0	18.761244	8
CCGTATG	350	3.4098775E-8	18.700226	9
GTATAGG	705	0.0	18.57849	1
>>END_MODULE