FastQCFastQC Report
Mon 15 Aug 2016
SRR2921143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815745
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91350.031.5423161
GGTATCA67350.031.0902751
TCAACGC111100.025.7685174
CAACGCA111050.025.7310875
ATCAACG112350.025.3756433
TATCAAC114650.024.9172692
AACGCAG115900.024.7916766
CATGGGG74950.024.5894624
GTACATG133950.024.0495011
ACATGGG133350.023.213283
TACATGG135300.023.1419812
CTATACG1300.00202436922.9397584
ACGCAGA131250.021.5753367
CGCAGAG133850.021.1569948
GAGTACT59850.019.38383512-13
GTACTTT60000.019.13740314-15
GCAGAGT145950.019.077219
AGTACTT64550.018.6637112-13
GTATATA11250.018.0294061
CGTTATT3053.4709174E-617.5987452
Produced by FastQC (version 0.11.5)