##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921143_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2815745
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.647983393382567	31.0	31.0	34.0	27.0	34.0
2	30.767300305958102	31.0	31.0	34.0	27.0	34.0
3	30.87607755673898	31.0	31.0	34.0	27.0	34.0
4	34.38741967046022	37.0	35.0	37.0	30.0	37.0
5	34.01178089635248	37.0	35.0	37.0	30.0	37.0
6	33.87746617680223	37.0	35.0	37.0	29.0	37.0
7	33.700266181774275	37.0	35.0	37.0	28.0	37.0
8	33.6970716453372	37.0	35.0	37.0	28.0	37.0
9	35.12247913074515	39.0	35.0	39.0	28.0	39.0
10-11	35.082609753369	39.0	35.0	39.0	28.0	39.0
12-13	34.95667540917235	39.0	35.0	39.0	27.0	39.0
14-15	36.07603937856588	40.0	36.0	41.0	27.0	41.0
16-17	36.0194236338873	40.0	36.0	41.0	27.0	41.0
18-19	36.05673276521844	40.0	36.0	41.0	27.0	41.0
20-21	36.04905238222922	40.0	36.0	41.0	27.0	41.0
22-23	36.007347611378165	40.0	36.0	41.0	27.0	41.0
24-25	35.906918950402115	40.0	36.0	41.0	27.0	41.0
26-27	35.806739956920815	40.0	35.5	41.0	27.0	41.0
28-29	35.71466503536364	39.5	35.0	41.0	27.0	41.0
30-31	35.635843444630105	39.0	35.0	41.0	26.5	41.0
32-33	35.50302033742402	39.0	35.0	41.0	26.0	41.0
34-35	35.38422690975213	39.0	35.0	41.0	25.0	41.0
36-37	35.25469085446303	39.0	35.0	41.0	25.0	41.0
38-39	35.08592131034593	39.0	35.0	41.0	25.0	41.0
40-41	34.944603470839866	39.0	34.0	41.0	24.0	41.0
42-43	34.81208436843535	39.0	34.0	41.0	24.0	41.0
44-45	34.64212331017191	39.0	34.0	41.0	23.5	41.0
46-47	34.433981060074686	39.0	34.0	41.0	22.5	41.0
48-49	34.24199101836281	38.5	33.0	41.0	21.5	41.0
50-51	34.03017371956622	38.0	33.0	40.5	19.5	41.0
52-53	33.857474487213864	38.0	33.0	40.0	19.0	41.0
54-55	33.6474433941994	38.0	33.0	40.0	18.0	41.0
56-57	33.375689204810804	38.0	33.0	40.0	16.5	41.0
58-59	33.14972076661772	37.0	32.0	40.0	14.5	41.0
60-61	32.8747292457236	37.0	32.0	40.0	13.0	41.0
62-63	32.579386450122435	36.5	32.0	40.0	12.0	41.0
64-65	32.27969702512124	36.0	31.5	39.0	8.0	41.0
66-67	31.9344038611451	35.5	31.0	39.0	7.5	41.0
68-69	31.564807004895684	35.0	31.0	39.0	7.0	40.0
70-71	31.201841430953444	35.0	31.0	38.0	5.5	40.0
72-73	31.001013230956637	35.0	31.0	37.0	2.0	39.5
74-75	30.79975779056697	35.0	31.0	37.0	2.0	39.0
76-77	30.56243445340398	35.0	31.0	36.0	2.0	39.0
78-79	30.235337006724684	35.0	30.0	36.0	2.0	38.5
80-81	29.945572308571975	35.0	30.0	35.5	2.0	37.0
82-83	29.727335927081462	34.5	30.0	35.0	2.0	37.0
84-85	29.568926696131932	34.0	30.0	35.0	2.0	36.0
86-87	29.366252093140538	34.0	30.0	35.0	2.0	36.0
88-89	29.21825360606163	34.0	30.0	35.0	2.0	36.0
90-91	29.06861771928921	34.0	29.0	35.0	2.0	35.5
92-93	28.951603039337726	34.0	29.0	35.0	2.0	35.0
94-95	28.85070416532747	34.0	29.0	35.0	2.0	35.0
96-97	28.787318809054085	34.0	29.0	35.0	2.0	35.0
98-99	28.743978591811405	34.0	29.0	35.0	2.0	35.0
100-101	28.601685877094695	34.0	29.0	35.0	2.0	35.0
102-103	27.229392576387422	32.0	26.0	34.0	2.0	35.0
104-105	28.322499551628432	34.0	29.0	35.0	2.0	35.0
106-107	28.60811898804757	34.0	29.0	35.0	2.0	35.0
108-109	28.81111588584904	34.0	30.0	35.0	2.0	35.0
110-111	28.786662854768455	34.0	30.0	35.0	2.0	35.0
112-113	28.711943020408455	34.0	30.0	35.0	2.0	35.0
114-115	28.594058588402007	34.0	29.5	35.0	2.0	35.0
116-117	28.491280637983905	34.0	29.0	35.0	2.0	35.0
118-119	28.3541284810947	34.0	29.0	35.0	2.0	35.0
120-121	28.183136612157703	34.0	29.0	35.0	2.0	35.0
122-123	27.997597438688516	34.0	29.0	35.0	2.0	35.0
124-125	27.209596572132774	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67163.0
3	23086.0
4	11576.0
5	9333.0
6	10000.0
7	11849.0
8	13934.0
9	15072.0
10	16427.0
11	17439.0
12	18611.0
13	19511.0
14	21037.0
15	17799.0
16	13600.0
17	12510.0
18	11941.0
19	11513.0
20	11646.0
21	11972.0
22	12927.0
23	15775.0
24	21418.0
25	28188.0
26	35794.0
27	42474.0
28	50256.0
29	59957.0
30	70998.0
31	86316.0
32	105953.0
33	134000.0
34	176197.0
35	254045.0
36	443994.0
37	695018.0
38	236129.0
39	287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.940745221655405	19.37159041917785	13.597414538564584	25.090249820602157
2	16.256709599103893	20.43532397499487	37.51646990384773	25.791496522053514
3	18.932292610026373	24.040942787919597	29.512198240316735	27.514566361737298
4	13.488328072118025	16.64374076678062	35.52270340124049	34.34522775986086
5	14.257261906285052	37.062199419701436	34.089697496982645	14.590841177030867
6	33.26402647062793	35.65973053434952	16.81485833056887	14.261384664453677
7	28.58530645850479	31.65701312046397	20.92181036047444	18.835870060556807
8	26.613611872188287	34.50782715682799	19.782102637798378	19.09645833318534
9	26.317157961628233	15.447099313133092	19.358763192004112	38.87697953323456
10-11	25.44977307545716	25.641762925037288	27.483089441694936	21.42537455781061
12-13	26.00926998306169	23.03525254105769	27.525548221796875	23.429929254083746
14-15	23.376573320561395	24.623080679020493	24.84643567171949	27.153910328698622
16-17	23.321059755096822	27.430541493930704	25.782755889008484	23.465642861963985
18-19	23.367856045441386	26.564028056290194	27.370086907715834	22.698028990552583
20-21	24.280120532752637	25.750421180798238	27.001166073564765	22.968292212884357
22-23	23.818123331558873	25.621358604204957	26.820460325893215	23.74005773834296
24-25	23.575272274114973	25.81392713448057	27.02261532351401	23.588185267890445
26-27	23.35562745169435	25.9250963803955	27.024130400610296	23.695145767299845
28-29	23.309364836309012	25.975656608518356	27.19451356289639	23.52046499227624
30-31	23.73176265217809	25.89007564654985	26.88794438507005	23.49021731620201
32-33	23.507394954699574	25.864420206067646	26.80378459696742	23.82440024226536
34-35	23.508555361854462	26.040235555527925	26.994979658424946	23.456229424192664
36-37	23.783780423362654	25.73137297566131	26.828776582281293	23.65607001869474
38-39	23.618043411263052	25.851932049001313	26.931218575949284	23.598805963786347
40-41	23.64786494333621	25.842780850868063	26.971545011486935	23.53780919430879
42-43	23.79074070850564	25.684190969201413	26.81365358812377	23.711414734169175
44-45	23.698900391142953	25.7737289275088	26.730173887295912	23.79719679405234
46-47	23.740429337627127	25.76846390702392	26.633569189860808	23.857537565488155
48-49	23.658752973962343	25.93371538592018	26.583044928922412	23.824486711195064
50-51	23.547537986675554	26.157149726198238	26.48458569509448	23.810726592031727
52-53	23.748905189353618	25.959574555383874	26.3947456904292	23.896774564833308
54-55	23.734688233326626	25.941664435953427	26.30235611277894	24.02129121794101
56-57	23.699581311115043	26.01814294818645	26.186918689950684	24.095357050747822
58-59	23.50537715386326	25.943852666623563	26.51181609757241	24.038954081940762
60-61	23.53106466573002	26.152595989719412	26.448793345683768	23.86754599886679
62-63	23.332405537572974	26.21217226591704	26.40438052225724	24.051041674252744
64-65	23.350430988084103	26.071803755207725	26.368377841354178	24.209387415353998
66-67	23.437890232131164	26.19341978257309	26.204841427424952	24.163848557870793
68-69	23.64035498277325	25.8852055637864	26.27457256398729	24.19986688945306
70-71	23.431108392077952	25.77442706679087	26.43232414096434	24.362140400166837
72-73	23.502779855714323	25.83511583872023	26.329871000554427	24.332233305011023
74-75	23.423315572761567	25.90090290109864	26.2096732653958	24.466108260743997
76-77	23.374741009619363	25.734310768565166	26.37794604333029	24.513002178485177
78-79	23.409744278148533	25.857607235760778	26.289825439615733	24.442823046474956
80-81	23.31418385347877	25.856327036929414	26.245148391891288	24.584340717700528
82-83	23.312204248764775	25.71419893665431	26.37981128047297	24.593785534107948
84-85	23.251745177838973	25.726740804349156	26.201120146203323	24.820393871608548
86-87	23.17704332641807	25.67259570445945	26.296799044211944	24.853561924910537
88-89	23.268779040462793	25.58111118254736	26.182868822091653	24.96724095489819
90-91	23.037580850209057	25.708546030050044	26.32561663547332	24.92825648426758
92-93	23.11901427037373	25.597209794350746	26.3730878789741	24.910688056301424
94-95	23.000766714128233	25.545611143267205	26.445492344314143	25.008129798290422
96-97	23.1433507885391	25.38132200627592	26.404479814265812	25.07084739091917
98-99	22.911659032744463	25.460406317655227	26.275758937372238	25.35217571222807
100-101	22.814180305959432	25.44585413157126	26.39564797703928	25.344317585430034
102-103	22.752699286317487	25.23908852522486	26.521283935883126	25.486928252574526
104-105	22.689057869792745	25.370425460684114	26.339258518582326	25.601258150940815
106-107	22.57916821031342	25.461788660048313	26.35295383836127	25.606089291276994
108-109	22.518398251868597	25.46829895689664	26.355098426518346	25.65820436471642
110-111	22.48436904504393	25.494963960805666	26.345142993535216	25.675524000615184
112-113	22.358222923901625	25.530981977238238	26.325752295133935	25.7850428037262
114-115	22.293666091634975	25.60093297915075	26.269465035835594	25.835935893378682
116-117	22.19401345969975	25.53604738882677	26.332082644886405	25.93785650658707
118-119	22.22906505635127	25.495316713415185	26.203336947131117	26.072281283102434
120-121	22.03696107313903	25.579765348505596	26.303928201685927	26.079345376669455
122-123	21.964495074324653	25.51786451897727	26.210375013363276	26.30726539333481
124-125	21.828961070273	25.716408143743045	26.168034836676867	26.286595949307085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	63.5
2	110.0
3	166.5
4	145.0
5	113.5
6	177.0
7	360.5
8	571.5
9	632.5
10	510.5
11	379.0
12	386.0
13	441.5
14	504.0
15	609.0
16	668.5
17	748.0
18	860.5
19	981.5
20	1155.0
21	1390.0
22	1685.0
23	2089.0
24	2679.0
25	3420.0
26	4782.0
27	6664.0
28	8876.5
29	12114.5
30	16204.0
31	21222.0
32	27461.0
33	33124.5
34	38936.5
35	45936.0
36	53596.5
37	60892.5
38	67395.5
39	73292.5
40	78425.5
41	83157.5
42	87638.0
43	93466.5
44	98942.0
45	103511.5
46	108091.0
47	112118.5
48	116041.0
49	120142.5
50	124902.0
51	124731.5
52	119146.0
53	115080.5
54	112090.5
55	106581.0
56	99292.5
57	90082.0
58	81478.5
59	73774.0
60	63434.0
61	53025.5
62	42787.0
63	31452.5
64	22130.0
65	16343.5
66	12048.0
67	8919.5
68	6666.5
69	5000.5
70	3846.5
71	2788.5
72	2010.5
73	1613.5
74	1196.5
75	860.0
76	614.0
77	362.5
78	215.5
79	116.0
80	67.0
81	50.5
82	39.5
83	25.5
84	22.0
85	13.5
86	5.5
87	3.0
88	2.0
89	3.5
90	4.0
91	2.0
92	1.0
93	2.5
94	3.0
95	2.0
96	1.5
97	0.5
98	1.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07692457946298405
2	1.0654373886839896E-4
3	3.9066037585079615E-4
4	0.18613191180309296
5	0.0717749654176781
6	0.008772101166831515
7	0.01321142361968147
8	0.010334742670234699
9	0.017686260652154225
10-11	0.017473173174417427
12-13	0.018804969910272414
14-15	0.025552740038604346
16-17	0.026813507615213734
18-19	0.02450505993973176
20-21	0.026369575369928736
22-23	0.02857147930654232
24-25	0.026671449296722535
26-27	0.025836856675586748
28-29	0.025943400414455146
30-31	0.026476119108797137
32-33	0.023084476754819772
34-35	0.024771419286902754
36-37	0.027790158554840728
38-39	0.032762199702032674
40-41	0.028695780335222118
42-43	0.027630342946538128
44-45	0.02803876061220032
46-47	0.02938831463786671
48-49	0.024416273490674757
50-51	0.029956547911831506
52-53	0.027008837803139132
54-55	0.031093014459761092
56-57	0.027239682570687332
58-59	0.02439851620086336
60-61	0.025925643124643745
62-63	0.02945934379711231
64-65	0.033170617367694874
66-67	0.03246032577523888
68-69	0.02810978977144592
70-71	0.036189356635632844
72-73	0.03979408646734701
74-75	0.02514432237294215
76-77	0.01726008569668063
78-79	0.021876981047644583
80-81	0.024824691156336953
82-83	0.023723739188030165
84-85	0.01731335756611483
86-87	0.016922697190264034
88-89	0.017473173174417427
90-91	0.018041406448382223
92-93	0.021734922729153387
94-95	0.01720681382724643
96-97	0.01914235841668901
98-99	0.01633670662648784
100-101	0.017846076260456825
102-103	0.014827336992518852
104-105	0.017846076260456825
106-107	0.015697444193277447
108-109	0.01406377353062866
110-111	0.012003927912506282
112-113	0.009855295845326902
114-115	0.008807615746454313
116-117	0.008132838733621119
118-119	0.005238400494362948
120-121	0.0028411663698239722
122-123	0.006818799287577533
124-125	0.05627285141232604
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2815745.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.487641129905526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.11447517878146	34.203123445293
2	14.769402061853473	14.618057378805826
3	6.332802184383747	9.40186325542394
4	3.1754784851959963	6.2858775876446185
5	1.9116305017562836	4.730104212194809
6	1.1687795647518326	3.470408619624352
7	0.7633296985721745	2.644277033071711
8	0.5543156832029977	2.194542048242268
9	0.3807442403410273	1.6957920895447727
>10	1.7167092325998858	14.328920442194693
>50	0.07632180001643395	2.5751268129780125
>100	0.03406778091716442	2.8447226342360006
>500	0.00129572508510487	0.3893297051669158
>1k	6.47862542410151E-4	0.6178547355791484
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.130874777367979E-4
2	0.0	0.0	0.0	0.0	2.130874777367979E-4
3	0.0	0.0	0.0	0.0	2.130874777367979E-4
4	0.0	0.0	0.0	0.0	2.130874777367979E-4
5	0.0	0.0	0.0	0.0	2.4860205735959754E-4
6	0.0	0.0	0.0	0.0	2.4860205735959754E-4
7	0.0	0.0	0.0	0.0	2.4860205735959754E-4
8	0.0	0.0	0.0	0.0	2.4860205735959754E-4
9	0.0	0.0	0.0	0.0	2.4860205735959754E-4
10-11	0.0	0.0	0.0	0.0	3.373885064165967E-4
12-13	0.0	0.0	0.0	3.551457962279965E-5	0.0012785248664207874
14-15	0.0	0.0	0.0	3.551457962279965E-5	0.0015626415034031846
16-17	0.0	0.0	0.0	7.10291592455993E-5	0.001722457111705783
18-19	0.0	0.0	0.0	8.878644905699912E-5	0.0019355445894425807
20-21	0.0	0.0	0.0	1.9533018792539808E-4	0.0021841466468021783
22-23	0.0	0.0	0.0	3.9066037585079615E-4	0.002414991414350376
24-25	0.0	0.0	0.0	8.345926211357918E-4	0.002450505993973176
26-27	0.0	0.0	0.0	0.0019888164588767803	0.002645836181898574
28-29	0.0	0.0	0.0	0.0053094296536085476	0.002734622630955573
30-31	0.0	0.0	0.0	0.016798396161584232	0.002734622630955573
32-33	0.0	0.0	0.0	0.040504378059803	0.0028056517902011724
34-35	0.0	0.0	0.0	0.07482921926523886	0.0028766809494467716
36-37	0.0	0.0	0.0	0.12985906039076692	0.0028944382392581715
38-39	0.0	0.0	0.0	0.2120753122175481	0.0029477101086923707
40-41	0.0	0.0	0.0	0.3045552775553184	0.0029477101086923707
42-43	0.0	0.0	0.0	0.39288003707722113	0.0029832246883151705
44-45	0.0	0.0	0.0	0.4990863874392035	0.0031075257169949693
46-47	0.0	0.0	0.0	0.6251986596797651	0.003267341325297568
48-49	0.0	0.0	0.0	0.752838058844107	0.0034981860928457653
50-51	0.0	0.0	0.0	0.8835672264356325	0.003622487121525564
52-53	0.0	0.0	0.0	1.016338482355469	0.003729030860393963
54-55	0.0	0.0	0.0	1.164860454338017	0.0038000600196395626
56-57	0.0	0.0	0.0	1.3363958739161395	0.003906603758507961
58-59	0.0	0.0	0.0	1.516117404097317	0.003977632917753561
60-61	0.0	0.0	0.0	1.6967623133486875	0.00404866207699916
62-63	0.0	0.0	0.0	1.8818643023427193	0.004332778713981558
64-65	1.7757289811399826E-5	0.0	0.0	2.0862151934923086	0.004368293293604357
66-67	3.551457962279965E-5	0.0	0.0	2.326027392395263	0.004457079742661356
68-69	7.10291592455993E-5	0.0	0.0	2.59517463406665	0.004510351612095556
70-71	7.10291592455993E-5	0.0	0.0	2.877178153561491	0.004599138061152554
72-73	7.10291592455993E-5	0.0	0.0	3.164970549534848	0.004829982828700752
74-75	7.10291592455993E-5	0.0	0.0	3.4694015260614863	0.00500755572681475
76-77	7.10291592455993E-5	0.0	0.0	3.8167696293520894	0.00504307030643755
78-79	7.10291592455993E-5	0.0	0.0	4.194982145045095	0.00511409946568315
80-81	7.10291592455993E-5	0.0	0.0	4.591058494288368	0.005202885914740149
82-83	7.10291592455993E-5	0.0	0.0	5.004217356330208	0.005433730682288347
84-85	7.10291592455993E-5	0.0	0.0	5.43072970031022	0.005451487972099746
86-87	7.10291592455993E-5	0.0	0.0	5.898101568146263	0.005735604609082143
88-89	7.10291592455993E-5	0.0	0.0	6.406457260867017	0.006001963956253141
90-91	7.10291592455993E-5	0.0	0.0	6.934789904625596	0.0060729931154987395
92-93	7.10291592455993E-5	0.0	0.0	7.486171510559372	0.0064281389117267365
94-95	7.10291592455993E-5	0.0	0.0	8.044638275127898	0.0064458962015381364
96-97	7.10291592455993E-5	0.0	0.0	8.641585086717726	0.0073870325615423265
98-99	7.10291592455993E-5	0.0	0.0	9.292212185407415	0.007990780415129921
100-101	7.10291592455993E-5	0.0	0.0	9.980928670742557	0.007990780415129921
102-103	7.10291592455993E-5	0.0	0.0	10.676925644900372	0.00807956686418692
104-105	1.0654373886839895E-4	0.0	0.0	11.374929192806878	0.00813283873362112
106-107	1.0654373886839895E-4	0.0	0.0	12.099355587952743	0.00813283873362112
108-109	1.0654373886839895E-4	0.0	0.0	12.863380739378034	0.00816835331324392
110-111	1.0654373886839895E-4	0.0	0.0	13.663488703700086	0.00820386789286672
112-113	1.0654373886839895E-4	0.0	0.0	14.479773559040325	0.00820386789286672
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9135	0.0	31.542316	1
GGTATCA	6735	0.0	31.090275	1
TCAACGC	11110	0.0	25.768517	4
CAACGCA	11105	0.0	25.731087	5
ATCAACG	11235	0.0	25.375643	3
TATCAAC	11465	0.0	24.917269	2
AACGCAG	11590	0.0	24.791676	6
CATGGGG	7495	0.0	24.589462	4
GTACATG	13395	0.0	24.049501	1
ACATGGG	13335	0.0	23.21328	3
TACATGG	13530	0.0	23.141981	2
CTATACG	130	0.002024369	22.939758	4
ACGCAGA	13125	0.0	21.575336	7
CGCAGAG	13385	0.0	21.156994	8
GAGTACT	5985	0.0	19.383835	12-13
GTACTTT	6000	0.0	19.137403	14-15
GCAGAGT	14595	0.0	19.07721	9
AGTACTT	6455	0.0	18.66371	12-13
GTATATA	1125	0.0	18.029406	1
CGTTATT	305	3.4709174E-6	17.598745	2
>>END_MODULE