FastQCFastQC Report
Mon 15 Aug 2016
SRR2921142_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921142_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147864
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT41850.1329472937839754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72700.031.691471
TCAACGC78950.028.7918784
ATCAACG80650.028.187223
CAACGCA81750.027.8785255
AACGCAG83800.027.3359436
TATCAAC86100.026.2647762
TACATGG123750.026.1605572
CATGGGG77550.024.7844644
GTACATG135500.024.60451
ACATGGG131650.024.2290953
ACGCAGA93200.023.8766337
CGCAGAG95000.022.9242738
GTATAAT31800.022.840131
GGTATCA56250.022.4377421
GTGTAGC35250.020.604711
TAGCCCT39150.020.3672224
GTAGCCC31950.018.9986423
ATGGGGG37800.017.946175
TAATGGA36050.017.8269584
GCAGAGT111200.017.8187629
Produced by FastQC (version 0.11.5)