FastQCFastQC Report
Mon 15 Aug 2016
SRR2921142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147864
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT43710.13885606239659656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85350.038.5117721
GGTATCA60500.033.5250171
TCAACGC101300.031.6154674
ATCAACG102800.031.2121683
CAACGCA103800.030.8490725
TATCAAC105450.030.4263382
AACGCAG107300.029.7731446
ACGCAGA123550.025.664617
CGCAGAG125100.025.0616918
TACATGG127700.023.3503362
GTATAAT28700.023.2799241
GTACATG135800.022.8427831
CATGGGG78650.022.2937624
ACATGGG133150.022.0821083
GCAGAGT143900.021.2090179
GTGTAGC34200.020.2337931
AGAGTAC125050.019.50581610-11
GAGTACT59850.019.28419512-13
GTAGCCC30600.018.9053653
GTATAGA13700.018.723771
Produced by FastQC (version 0.11.5)