FastQCFastQC Report
Mon 15 Aug 2016
SRR2921141_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921141_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699270
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74950.030.106651
CATGGGG88450.026.5747244
GTACATG143550.026.3784221
GGTATCA57700.025.8995841
TACATGG145900.025.2889062
ACATGGG142600.025.164683
TCAACGC88300.024.5307144
CAACGCA89900.023.9617425
ATCAACG90450.023.9489483
TATCAAC91750.023.674482
AACGCAG93550.023.4058956
ATGGGGA49900.020.9885125
ACGCAGA106950.020.251167
GTATACG2107.1153816E-519.846031
CGCAGAG109850.019.55448
GCGTAAA1959.870548E-418.319411
ATGGGGG46400.018.0830065
TAAGGTG11150.017.07835
GCAGAGT120550.017.0290039
AGAGTAC108950.016.27670910-11
Produced by FastQC (version 0.11.5)