FastQCFastQC Report
Mon 15 Aug 2016
SRR2921141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699270
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29290.10851081959196375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.10391698496260099No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72300.035.886491
GTATCAA103200.035.2557681
TCAACGC125350.028.5410394
TATCAAC128300.028.2090722
ATCAACG128000.027.9967353
CAACGCA127950.027.958355
AACGCAG132550.027.1521036
CATGGGG86650.025.8051014
ACGCAGA151000.023.7163247
GTACATG153900.023.3312441
CGCAGAG154650.023.1185428
ACATGGG151100.023.006313
TACATGG158000.022.5291062
GAGTACT62750.021.71069512-13
GTACTTT62000.021.58951214-15
GCAGAGT163850.020.8768679
GTATAAT12300.020.8517911
ATGGGGA50000.019.5232075
AGTACTT67750.019.49376112-13
AGAGTAC153850.019.04411110-11
Produced by FastQC (version 0.11.5)