##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921141_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2699270 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.843369874077066 31.0 31.0 34.0 27.0 34.0 2 30.915494189169664 31.0 31.0 34.0 27.0 34.0 3 31.00116438889033 31.0 31.0 34.0 27.0 34.0 4 34.538869768492965 37.0 35.0 37.0 31.0 37.0 5 34.24291049061413 37.0 35.0 37.0 30.0 37.0 6 34.13399215343408 37.0 35.0 37.0 30.0 37.0 7 33.96710703264216 37.0 35.0 37.0 29.0 37.0 8 33.946741526412694 37.0 35.0 37.0 29.0 37.0 9 35.3834355214558 39.0 35.0 39.0 29.0 39.0 10-11 35.343860006594376 39.0 35.0 39.0 29.0 39.0 12-13 35.24759731334768 39.0 35.0 39.0 29.0 39.0 14-15 36.36012829394614 40.0 36.0 41.0 28.5 41.0 16-17 36.32256943543995 40.0 36.0 41.0 28.0 41.0 18-19 36.36276085756519 40.0 36.0 41.0 28.5 41.0 20-21 36.357567231140266 40.0 36.0 41.0 28.0 41.0 22-23 36.33333419776458 40.0 36.0 41.0 28.0 41.0 24-25 36.249481341251524 40.0 36.0 41.0 28.0 41.0 26-27 36.14875521900366 40.0 36.0 41.0 27.5 41.0 28-29 36.05067647919623 40.0 36.0 41.0 27.0 41.0 30-31 35.95661641851315 39.5 35.0 41.0 27.0 41.0 32-33 35.82609094310684 39.0 35.0 41.0 27.0 41.0 34-35 35.715299321668454 39.0 35.0 41.0 27.0 41.0 36-37 35.580130924286934 39.0 35.0 41.0 26.5 41.0 38-39 35.41483178785376 39.0 35.0 41.0 26.0 41.0 40-41 35.26331063583858 39.0 35.0 41.0 25.0 41.0 42-43 35.13963238208849 39.0 34.5 41.0 25.0 41.0 44-45 34.95517639954507 39.0 34.0 41.0 24.0 41.0 46-47 34.72550133925098 39.0 34.0 41.0 24.0 41.0 48-49 34.51039280990786 39.0 34.0 41.0 23.0 41.0 50-51 34.29647200909876 38.0 33.0 41.0 22.5 41.0 52-53 34.12200168934564 38.0 33.0 40.0 22.0 41.0 54-55 33.90646026518281 38.0 33.0 40.0 21.5 41.0 56-57 33.63702741852427 38.0 33.0 40.0 20.0 41.0 58-59 33.41311224886729 37.0 33.0 40.0 19.5 41.0 60-61 33.1305986433369 37.0 32.0 40.0 18.5 41.0 62-63 32.81584798853024 36.0 32.0 39.5 18.0 41.0 64-65 32.513650542554096 36.0 32.0 39.0 16.5 41.0 66-67 32.17579845661975 35.5 31.5 39.0 13.5 41.0 68-69 31.81253542624487 35.0 31.0 38.5 12.5 40.0 70-71 31.450168378857988 35.0 31.0 37.5 9.5 40.0 72-73 31.24602429545766 35.0 31.0 37.0 8.0 39.0 74-75 31.04759953617089 35.0 31.0 37.0 8.0 39.0 76-77 30.81485549796797 35.0 31.0 36.0 7.0 39.0 78-79 30.501899587666294 35.0 31.0 36.0 7.0 37.5 80-81 30.221838311839868 35.0 30.0 35.0 6.0 37.0 82-83 30.008178507522402 35.0 30.0 35.0 2.0 37.0 84-85 29.85213150222097 34.0 30.0 35.0 2.0 36.0 86-87 29.666163629425732 34.0 30.0 35.0 2.0 36.0 88-89 29.52044886209975 34.0 30.0 35.0 2.0 36.0 90-91 29.373154037943593 34.0 30.0 35.0 2.0 35.0 92-93 29.26171020312899 34.0 30.0 35.0 2.0 35.0 94-95 29.17294064691565 34.0 30.0 35.0 2.0 35.0 96-97 29.10435543684033 34.0 29.5 35.0 2.0 35.0 98-99 29.056416920130257 34.0 29.0 35.0 2.0 35.0 100-101 28.930883164707495 34.0 29.0 35.0 2.0 35.0 102-103 27.557208615662752 32.5 26.0 34.0 2.0 35.0 104-105 28.649321297980563 34.0 29.0 35.0 2.0 35.0 106-107 28.938471883138774 34.0 29.5 35.0 2.0 35.0 108-109 29.147473576189117 34.0 30.0 35.0 2.0 35.0 110-111 29.136759938798267 34.0 30.0 35.0 2.0 35.0 112-113 29.067493433409773 34.0 30.0 35.0 2.0 35.0 114-115 28.962970358652527 34.0 30.0 35.0 2.0 35.0 116-117 28.872698173950734 34.0 30.0 35.0 2.0 35.0 118-119 28.73831424792629 34.0 30.0 35.0 2.0 35.0 120-121 28.587042978286725 34.0 30.0 35.0 2.0 35.0 122-123 28.411234148492 34.0 30.0 35.0 2.0 35.0 124-125 27.62371678268569 33.5 27.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51316.0 3 17840.0 4 9344.0 5 7567.0 6 8590.0 7 10414.0 8 12197.0 9 13378.0 10 14762.0 11 16078.0 12 17146.0 13 18977.0 14 21253.0 15 17642.0 16 12527.0 17 11415.0 18 10771.0 19 10451.0 20 10417.0 21 10862.0 22 12242.0 23 15059.0 24 20423.0 25 27309.0 26 34351.0 27 41296.0 28 49183.0 29 58617.0 30 69727.0 31 83838.0 32 103176.0 33 131243.0 34 173420.0 35 250432.0 36 436619.0 37 671166.0 38 217987.0 39 235.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.950017536795755 19.291253921679854 14.226901667144942 24.531826874379444 2 16.455701829942594 20.105102685360645 38.016830507564094 25.422364977132666 3 19.06992855436037 24.509911068053963 29.318608282655774 27.101552094929897 4 13.870286356717529 16.90221351059135 34.7347866382454 34.49271349444572 5 14.507922997304135 36.97328305400925 33.53653653950525 14.982257409181365 6 33.3661595278956 35.04444503724254 16.953506845336015 14.63588858952585 7 28.775268992500813 31.67397812490737 20.774298248214127 18.776454634377686 8 27.014900543862257 34.50941060655517 19.33673183948746 19.138957010095112 9 26.713944136305546 15.708463590926252 19.336902548855626 38.24068972391257 10-11 25.828988373236932 25.583677141443296 27.0114140772446 21.57592040807517 12-13 26.587199836370385 23.081817029781625 27.07482794064573 23.256155193202257 14-15 23.876092483925518 24.41344329366661 24.568615800100012 27.141848422307856 16-17 23.63336038449709 27.30355481936863 25.549898280213 23.513186515921277 18-19 23.801850665045116 26.11593234621069 27.285275627475425 22.796941361268765 20-21 24.775761779987334 25.307207751036565 26.82837758066184 23.088652888314265 22-23 24.351623486652077 25.102310388791523 26.795103331315566 23.750962793240834 24-25 23.95657431092642 25.531657863911743 26.927777388685264 23.583990436476572 26-27 23.652775768000865 25.673109543104268 26.99628902026911 23.67782566862576 28-29 23.70846394171176 25.695965558297317 26.98382376864354 23.61174673134738 30-31 23.943881700965505 25.543145759722226 26.840066182832857 23.672906356479416 32-33 23.723621126554672 25.55479448374659 26.808574341636344 23.913010048062393 34-35 23.80767894612509 25.732631120622173 26.86684360944745 23.59284632380529 36-37 24.06023911770248 25.399127048256183 26.761824101168575 23.778809732872762 38-39 23.990321749215234 25.61415998204876 26.68324796552355 23.712270303212453 40-41 24.013143388858357 25.489717970030494 26.96334651699084 23.53379212412031 42-43 24.03365732202208 25.47281856077576 26.640667528610724 23.852856588591433 44-45 23.952623818384016 25.479029500887336 26.54372067152141 24.02462600920724 46-47 24.223236282028857 25.465924823531854 26.42573440691583 23.885104487523456 48-49 24.047350390294245 25.7807274499232 26.199944045164074 23.971978114618476 50-51 23.91456367238426 26.027773680781664 26.21982809416058 23.837834552673492 52-53 24.212988834061374 25.711415404637428 26.100733575338587 23.974862185962607 54-55 24.04511128849479 25.720479143459173 26.04770015141846 24.18670941662757 56-57 23.962315995783655 25.89352301082194 25.893078326824583 24.251082666569822 58-59 23.870153350061578 25.79933629273674 26.021300812134456 24.30920954506723 60-61 23.881364131652198 25.815952320647977 26.300905595676 24.00177795202383 62-63 23.792494936071844 25.809135304158094 26.382213364619872 24.01615639515019 64-65 23.755844255952468 25.84823241831383 26.13964844039526 24.256274885338442 66-67 23.84097628133683 26.069720881150975 25.911645871955724 24.177656965556473 68-69 24.061646873799113 25.627924971030534 26.17861347525746 24.131814679912885 70-71 23.804670598209032 25.643446477131114 26.24474082858877 24.307142096071082 72-73 23.958045332477454 25.69451908273596 26.00101993997475 24.346415644811835 74-75 23.802873111942567 25.805925056890462 26.05379367947982 24.33740815168715 76-77 23.893733661629216 25.549015575527505 26.20132763447255 24.35592312837073 78-79 23.833940481338487 25.724621973014244 26.126762572706763 24.314674972940505 80-81 23.851047816821318 25.79252808203924 26.036023717386463 24.320400383752975 82-83 23.781797295857633 25.722387686378774 26.14550861314274 24.35030640462085 84-85 23.67405210565787 25.770724582262496 26.026020963204715 24.529202348874918 86-87 23.58977988271972 25.756734204474967 26.05076859117678 24.602717321628525 88-89 23.641641050419295 25.598097382251723 25.989270077752845 24.77099148957614 90-91 23.52850612086095 25.771904715587233 26.085752266084917 24.613836897466896 92-93 23.565406566335913 25.74462495284721 26.178375009356447 24.511593471460426 94-95 23.403505183594355 25.619191174754143 26.262050249684766 24.715253391966733 96-97 23.736664740444496 25.32262059839861 26.176266188464563 24.764448472692326 98-99 23.530267267923772 25.43712182126064 26.125911982777467 24.90669892803812 100-101 23.373241877719153 25.426046545579318 26.27809331977914 24.922618256922394 102-103 23.31058035653082 25.348177236080225 26.320279705251252 25.020962702137695 104-105 23.3169370661242 25.463773512895614 26.10172725874792 25.117562162232264 106-107 23.197961652975977 25.51866931643381 26.134949525789374 25.148419504800835 108-109 23.10045700337102 25.435179551859544 26.161534796208507 25.302828648560926 110-111 23.098606578514456 25.570162373173744 26.15182914358377 25.179401904728028 112-113 23.04330533108535 25.603022145716793 26.129159604662377 25.224512918535485 114-115 23.031912913013606 25.833363127783738 25.835048907050023 25.299675052152637 116-117 22.888927009241016 25.6973369187871 25.97159967899864 25.442136392973243 118-119 22.97205351110844 25.557432078841398 25.99355239195588 25.47696201809428 120-121 22.855557726986866 25.60356476983394 26.068500116238468 25.472377386940725 122-123 22.704437619519293 25.72941619102858 25.973294367164385 25.59285182228774 124-125 22.780041352243572 25.798966416314347 25.90405211954342 25.516940111898666 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 25.0 1 35.5 2 89.5 3 149.5 4 134.5 5 116.5 6 156.0 7 383.0 8 656.5 9 725.5 10 613.5 11 472.5 12 448.0 13 507.5 14 578.0 15 622.0 16 722.0 17 830.0 18 933.0 19 1070.5 20 1226.5 21 1439.0 22 1696.5 23 2037.5 24 2475.5 25 3168.5 26 4362.5 27 5957.5 28 7924.5 29 10552.5 30 14138.0 31 19453.0 32 26123.5 33 31804.5 34 37266.5 35 43804.5 36 51123.0 37 57577.5 38 62549.5 39 66352.5 40 69612.0 41 73467.0 42 77970.0 43 83883.0 44 90389.5 45 96601.5 46 102622.0 47 106129.0 48 108804.5 49 112556.0 50 116607.0 51 117386.5 52 114012.5 53 112280.5 54 111082.0 55 105606.5 56 98650.5 57 91141.0 58 84048.5 59 76016.5 60 64339.0 61 53769.0 62 43582.0 63 32803.5 64 24115.5 65 18404.0 66 14174.0 67 10706.5 68 7800.0 69 5541.5 70 4133.5 71 3043.0 72 2127.0 73 1639.0 74 1228.5 75 917.5 76 685.5 77 414.0 78 237.0 79 134.5 80 85.0 81 65.0 82 47.5 83 30.5 84 22.0 85 13.5 86 7.0 87 9.0 88 8.5 89 7.0 90 7.0 91 3.5 92 2.0 93 2.0 94 2.0 95 1.5 96 2.0 97 2.0 98 2.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07720605941606434 2 1.8523526731301426E-4 3 5.557058019390428E-4 4 0.1868282906119062 5 0.06690697855346076 6 0.007631693013296187 7 0.011484586573406884 8 0.008928339884487288 9 0.016708221111633885 10-11 0.016800838745290394 12-13 0.018227150303600604 14-15 0.024469578812049184 16-17 0.02519199635456994 18-19 0.022913602566619864 20-21 0.025525419835733365 22-23 0.027470390142520017 24-25 0.02530313751495775 26-27 0.024321390598198774 28-29 0.02522904340803254 30-31 0.025377231621882955 32-33 0.021876285069666986 34-35 0.024358437651661378 36-37 0.026840590233655766 38-39 0.03143442486301852 40-41 0.02693320786731227 42-43 0.02663683143961145 44-45 0.02745186661578871 46-47 0.028378042952353785 48-49 0.024636290552630898 50-51 0.03023039562548393 52-53 0.026155219744597613 54-55 0.029193078128531044 56-57 0.026840590233655766 58-59 0.023728637742797126 60-61 0.02443253175858658 62-63 0.0286003252731294 64-65 0.03312006579556695 66-67 0.03213831887880798 68-69 0.02737777250886351 70-71 0.03732490636357237 72-73 0.03971444131191026 74-75 0.024562196445705693 76-77 0.017967820929362385 78-79 0.021746620382547873 80-81 0.02441400823185528 82-83 0.023432261315096306 84-85 0.01652298584432087 86-87 0.016152515309694842 88-89 0.017115738699722518 90-91 0.018801379632270947 92-93 0.022080043863711298 94-95 0.017300973967035532 96-97 0.02039440293116287 98-99 0.01778258566204937 100-101 0.017578826868005052 102-103 0.015893185935456623 104-105 0.017967820929362385 106-107 0.015430097767174088 108-109 0.014226068529639495 110-111 0.011188210145706062 112-113 0.009650757427008043 114-115 0.008205922341966532 116-117 0.008002163547922216 118-119 0.004797593423407069 120-121 0.002871146643351721 122-123 0.006538804936149403 124-125 0.0545332626969514 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2699270.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.59694360735983 #Duplication Level Percentage of deduplicated Percentage of total 1 72.08819101785615 36.474421356870515 2 14.113930804669755 14.282435220041087 3 5.433304864957238 8.247258595615058 4 2.6272459304228772 5.317224567370878 5 1.526220559569351 3.8611047792461806 6 0.9379051914089678 2.8473081647261744 7 0.6355610332564265 2.251021203109753 8 0.4742580539357624 1.919680640825919 9 0.3228335956559764 1.470095391056998 >10 1.7037085201936017 15.242312523252167 >50 0.09414732338204511 3.271933288031788 >100 0.03924339354477064 3.32611395823676 >500 0.0029359243806663347 0.9614449728887066 >1k 5.137867665941997E-4 0.527645338728003 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2929 0.10851081959196375 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2805 0.10391698496260099 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.7047053462602854E-5 2 0.0 0.0 0.0 0.0 3.7047053462602854E-5 3 0.0 0.0 0.0 0.0 3.7047053462602854E-5 4 0.0 0.0 0.0 0.0 3.7047053462602854E-5 5 0.0 0.0 0.0 0.0 1.1114116038780856E-4 6 0.0 0.0 0.0 0.0 1.4818821385041142E-4 7 0.0 0.0 0.0 0.0 1.4818821385041142E-4 8 0.0 0.0 0.0 0.0 1.4818821385041142E-4 9 0.0 0.0 0.0 0.0 1.4818821385041142E-4 10-11 0.0 0.0 0.0 0.0 1.4818821385041142E-4 12-13 0.0 0.0 0.0 0.0 3.519470078947271E-4 14-15 0.0 0.0 0.0 0.0 4.075175880886314E-4 16-17 0.0 0.0 0.0 0.0 4.445646415512342E-4 18-19 0.0 0.0 0.0 1.8523526731301427E-5 5.927528554016457E-4 20-21 0.0 3.7047053462602854E-5 0.0 3.7047053462602854E-5 6.853704890581527E-4 22-23 0.0 3.7047053462602854E-5 0.0 1.2966468711911E-4 7.965116494459614E-4 24-25 0.0 3.7047053462602854E-5 0.0 6.297999088642485E-4 8.150351761772628E-4 26-27 0.0 9.261763365650713E-5 0.0 0.0014818821385041141 8.706057563711671E-4 28-29 0.0 1.1114116038780856E-4 0.0 0.0035750406591411755 8.891292831024684E-4 30-31 0.0 1.2966468711911E-4 0.0 0.009446998632963726 8.891292831024684E-4 32-33 0.0 1.4818821385041142E-4 0.0 0.024210249437810964 9.076528098337698E-4 34-35 0.0 1.4818821385041142E-4 0.0 0.04301162907008191 0.00103731749695288 36-37 0.0 1.4818821385041142E-4 0.0 0.07483504799445775 0.00103731749695288 38-39 0.0 1.4818821385041142E-4 0.0 0.12644159346786354 0.00103731749695288 40-41 0.0 1.4818821385041142E-4 0.0 0.18645782007728015 0.0010558410236841814 42-43 0.0 1.4818821385041142E-4 0.0 0.24321390598198772 0.0010743645504154827 44-45 0.0 1.4818821385041142E-4 0.0 0.31004679042852323 0.0010743645504154827 46-47 0.0 1.4818821385041142E-4 0.0 0.38658600288226075 0.0010743645504154827 48-49 0.0 1.4818821385041142E-4 0.0 0.46925650268405905 0.0011484586573406884 50-51 0.0 1.4818821385041142E-4 0.0 0.5541683492203449 0.001222552764265894 52-53 0.0 1.4818821385041142E-4 0.0 0.6425626187821152 0.0013707409781163055 54-55 0.0 1.4818821385041142E-4 0.0 0.7381254931888992 0.0014077880315789084 56-57 0.0 1.4818821385041142E-4 0.0 0.854694046908979 0.001481882138504114 58-59 0.0 1.4818821385041142E-4 0.0 0.9821544343470643 0.0015374527186980183 60-61 0.0 1.6671174058171284E-4 0.0 1.1166167148895814 0.0015559762454293198 62-63 0.0 1.8523526731301426E-4 0.0 1.2536167185942866 0.0016671174058171282 64-65 0.0 1.8523526731301426E-4 0.0 1.40297191462877 0.0016671174058171282 66-67 0.0 1.8523526731301426E-4 0.0 1.5758149425585435 0.0016671174058171282 68-69 0.0 1.8523526731301426E-4 0.0 1.7709602966728042 0.0016856409325484297 70-71 0.0 1.8523526731301426E-4 0.0 1.980998566279031 0.001741211512742334 72-73 0.0 1.8523526731301426E-4 0.0 2.1960567116294403 0.0018523526731301426 74-75 0.0 1.8523526731301426E-4 0.0 2.425359449036221 0.0019264467800553483 76-77 0.0 1.8523526731301426E-4 0.0 2.680872976767793 0.0019264467800553483 78-79 0.0 1.8523526731301426E-4 0.0 2.9669873706594747 0.0019449703067866498 80-81 0.0 1.8523526731301426E-4 0.0 3.2687541446391064 0.001963493833517951 82-83 0.0 1.8523526731301426E-4 0.0 3.5890073982965767 0.002037587940443157 84-85 0.0 1.8523526731301426E-4 0.0 3.924264708606401 0.002037587940443157 86-87 0.0 1.8523526731301426E-4 0.0 4.285251197546003 0.0021487291008309654 88-89 0.0 1.8523526731301426E-4 0.0 4.680487687411782 0.002259870261218774 90-91 0.0 1.8523526731301426E-4 0.0 5.0969521389116315 0.002259870261218774 92-93 0.0 1.8523526731301426E-4 0.0 5.526642388497631 0.0023339643681439797 94-95 0.0 1.8523526731301426E-4 0.0 5.976578852800943 0.002389534948337884 96-97 0.0 1.8523526731301426E-4 0.0 6.460932029771012 0.0026859113760387067 98-99 0.0 1.8523526731301426E-4 0.0 6.9708106265768155 0.002778529009695214 100-101 0.0 1.8523526731301426E-4 0.0 7.518162317960041 0.002778529009695214 102-103 0.0 1.8523526731301426E-4 0.0 8.074775772708918 0.0028526231166204194 104-105 0.0 1.8523526731301426E-4 0.0 8.646541472324 0.0028896701700830223 106-107 0.0 1.8523526731301426E-4 0.0 9.249519314481322 0.002908193696814324 108-109 0.0 1.8523526731301426E-4 0.0 9.87772620004668 0.0029452407502769265 110-111 3.7047053462602854E-5 1.8523526731301426E-4 0.0 10.530995417279486 0.0029637642770082282 112-113 3.7047053462602854E-5 1.8523526731301426E-4 0.0 11.199546544065617 0.0029637642770082282 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7230 0.0 35.88649 1 GTATCAA 10320 0.0 35.255768 1 TCAACGC 12535 0.0 28.541039 4 TATCAAC 12830 0.0 28.209072 2 ATCAACG 12800 0.0 27.996735 3 CAACGCA 12795 0.0 27.95835 5 AACGCAG 13255 0.0 27.152103 6 CATGGGG 8665 0.0 25.805101 4 ACGCAGA 15100 0.0 23.716324 7 GTACATG 15390 0.0 23.331244 1 CGCAGAG 15465 0.0 23.118542 8 ACATGGG 15110 0.0 23.00631 3 TACATGG 15800 0.0 22.529106 2 GAGTACT 6275 0.0 21.710695 12-13 GTACTTT 6200 0.0 21.589512 14-15 GCAGAGT 16385 0.0 20.876867 9 GTATAAT 1230 0.0 20.851791 1 ATGGGGA 5000 0.0 19.523207 5 AGTACTT 6775 0.0 19.493761 12-13 AGAGTAC 15385 0.0 19.044111 10-11 >>END_MODULE