FastQCFastQC Report
Mon 15 Aug 2016
SRR2921140_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921140_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283576
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63750.031.3751181
CATGGGG73600.028.7028264
TACATGG113300.028.5202662
GTACATG115100.028.290351
ACATGGG118350.026.800483
TCAACGC72650.026.6208464
TATCAAC74150.026.5644282
TACCGTA2957.2759576E-1226.221497
CAACGCA74550.026.1014545
ATCAACG74900.026.0600763
AACGCAG76450.025.4510866
GGTATCA48250.025.2919831
ACGCAGA86300.022.4082857
TAGGACG2204.1756466E-621.6392124
CGCAGAG90350.021.404298
ATGGGGG33950.021.0332365
ATGGGGA33250.020.5812035
GTATATA11650.020.4390351
GCAGAGT94350.019.6134749
GTATAAT14100.019.4207081
Produced by FastQC (version 0.11.5)