FastQCFastQC Report
Mon 15 Aug 2016
SRR2921140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283576
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23520.1029963530883141No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76150.040.7192041
GGTATCA51150.036.139481
TCAACGC95300.032.0307164
ATCAACG96950.031.484893
CAACGCA97550.031.3618435
TATCAAC99100.031.0992072
AACGCAG101550.030.1692666
ACGCAGA113150.026.3928687
CGCAGAG118800.024.9879748
CATGGGG71500.023.68114
GTACATG116300.023.3290771
TACATGG115900.022.9909292
GCAGAGT126750.022.90599
ACATGGG116750.022.2132663
GAGTACT47950.021.40306512-13
AGAGTAC116050.020.76277410-11
GTACTTT49850.020.22970414-15
TACCGTA2659.2636037E-720.2038467
AGTACTT53150.020.14859212-13
CAGAGTA120800.018.96135110-11
Produced by FastQC (version 0.11.5)