##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921140_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2283576
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.56160294205229	31.0	31.0	34.0	27.0	34.0
2	30.720096462740894	31.0	31.0	34.0	27.0	34.0
3	30.812522990257385	31.0	31.0	34.0	27.0	34.0
4	34.31602977085063	37.0	35.0	37.0	30.0	37.0
5	33.95246709546781	37.0	35.0	37.0	30.0	37.0
6	33.814719983044135	37.0	35.0	37.0	29.0	37.0
7	33.633417937480516	37.0	35.0	37.0	28.0	37.0
8	33.63347705528522	37.0	35.0	37.0	28.0	37.0
9	35.054853878303156	39.0	35.0	39.0	28.0	39.0
10-11	35.005430079839684	39.0	35.0	39.0	27.5	39.0
12-13	34.87843649609209	39.0	35.0	39.0	27.0	39.0
14-15	35.970073472483506	40.0	36.0	41.0	27.0	41.0
16-17	35.91581164804675	40.0	36.0	41.0	27.0	41.0
18-19	35.96368064824644	40.0	36.0	41.0	27.0	41.0
20-21	35.96434736571062	40.0	36.0	41.0	27.0	41.0
22-23	35.92513036570712	40.0	36.0	41.0	27.0	41.0
24-25	35.83051100554569	40.0	36.0	41.0	27.0	41.0
26-27	35.722609407350575	39.5	35.0	41.0	27.0	41.0
28-29	35.63217142762054	39.0	35.0	41.0	27.0	41.0
30-31	35.54265502877942	39.0	35.0	41.0	26.0	41.0
32-33	35.40760018497305	39.0	35.0	41.0	25.0	41.0
34-35	35.28850583470837	39.0	35.0	41.0	25.0	41.0
36-37	35.14972897770865	39.0	35.0	41.0	25.0	41.0
38-39	34.98301939589486	39.0	34.0	41.0	24.0	41.0
40-41	34.84174141784639	39.0	34.0	41.0	24.0	41.0
42-43	34.71208205901621	39.0	34.0	41.0	23.5	41.0
44-45	34.534145787133866	39.0	34.0	41.0	23.0	41.0
46-47	34.3117883967952	39.0	33.0	41.0	21.5	41.0
48-49	34.10977475678497	38.0	33.0	41.0	20.0	41.0
50-51	33.898039303268206	38.0	33.0	40.0	18.5	41.0
52-53	33.71588442863299	38.0	33.0	40.0	18.0	41.0
54-55	33.508195260416116	38.0	33.0	40.0	16.5	41.0
56-57	33.237641094493895	38.0	32.5	40.0	14.5	41.0
58-59	33.00627458862766	37.0	32.0	40.0	13.0	41.0
60-61	32.73966160968586	37.0	32.0	40.0	11.5	41.0
62-63	32.44006023885345	36.0	31.5	40.0	8.0	41.0
64-65	32.14765306694413	36.0	31.0	39.0	8.0	41.0
66-67	31.813259992222726	35.5	31.0	39.0	7.0	41.0
68-69	31.45664256411873	35.0	31.0	38.5	6.5	40.0
70-71	31.10260967885457	35.0	30.5	37.5	2.0	40.0
72-73	30.905222992359352	35.0	30.5	37.0	2.0	39.5
74-75	30.709627575346737	35.0	30.0	37.0	2.0	39.0
76-77	30.473379471495583	35.0	30.0	36.0	2.0	39.0
78-79	30.149150499041852	35.0	30.0	36.0	2.0	38.5
80-81	29.85600282188988	35.0	30.0	35.5	2.0	37.0
82-83	29.634445711463073	34.5	30.0	35.0	2.0	37.0
84-85	29.47259013932534	34.0	30.0	35.0	2.0	36.0
86-87	29.272981061282827	34.0	30.0	35.0	2.0	36.0
88-89	29.125037660231143	34.0	29.0	35.0	2.0	36.0
90-91	28.9676391326586	34.0	29.0	35.0	2.0	35.5
92-93	28.851193916909267	34.0	29.0	35.0	2.0	35.0
94-95	28.74821858348485	34.0	29.0	35.0	2.0	35.0
96-97	28.67999663685378	34.0	29.0	35.0	2.0	35.0
98-99	28.63347136245958	34.0	29.0	35.0	2.0	35.0
100-101	28.49231183897536	34.0	29.0	35.0	2.0	35.0
102-103	27.128799304249124	32.0	26.0	34.0	2.0	35.0
104-105	28.21846021327952	34.0	29.0	35.0	2.0	35.0
106-107	28.50024676209594	34.0	29.0	35.0	2.0	35.0
108-109	28.706652636041017	34.0	29.5	35.0	2.0	35.0
110-111	28.68811416830445	34.0	30.0	35.0	2.0	35.0
112-113	28.61569660917789	34.0	29.5	35.0	2.0	35.0
114-115	28.501476850343497	34.0	29.0	35.0	2.0	35.0
116-117	28.406669845890832	34.0	29.0	35.0	2.0	35.0
118-119	28.27388206041752	34.0	29.0	35.0	2.0	35.0
120-121	28.11036921915452	34.0	29.0	35.0	2.0	35.0
122-123	27.933920088492783	34.0	29.0	35.0	2.0	35.0
124-125	27.147895012033757	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56381.0
3	19077.0
4	9434.0
5	7681.0
6	8377.0
7	10012.0
8	11418.0
9	12479.0
10	13668.0
11	14315.0
12	15393.0
13	16143.0
14	17930.0
15	15062.0
16	11240.0
17	10175.0
18	9761.0
19	9398.0
20	9133.0
21	9378.0
22	10307.0
23	12991.0
24	17766.0
25	23713.0
26	29440.0
27	34608.0
28	41273.0
29	49015.0
30	58162.0
31	70366.0
32	86974.0
33	108746.0
34	143817.0
35	207433.0
36	359285.0
37	555194.0
38	187757.0
39	274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.617516300801555	19.46464044018628	14.400710271904249	24.517132987107917
2	16.21870638687706	20.406091681763623	38.57398033695441	24.80122159440491
3	19.121548981526693	24.15376834029248	29.515954220703154	27.208728457477672
4	13.43463125407177	16.72885367517543	35.769528585185235	34.06698648556757
5	14.25031397043551	37.10898301623707	33.97462470344802	14.6660783098794
6	33.032681885367055	35.752603054688784	16.864860130289593	14.349854929654567
7	28.557770215524226	31.834693225067557	20.733159021929076	18.87437753747914
8	26.264500184598568	35.2418591593955	19.456469993645253	19.03717066236068
9	26.448630918463035	15.928797335988119	19.299676371435716	38.32289537411313
10-11	25.567044670898053	25.818886896780384	27.240941399379942	21.373127032941618
12-13	26.105896816981122	23.09527157033691	27.500134682523726	23.29869693015824
14-15	23.442923644502944	24.554347457098146	25.04228587948387	26.960443018915033
16-17	23.309094365524103	27.581415029503326	25.706636507030556	23.402854097942015
18-19	23.284355809410343	26.57460293821342	27.46574712442291	22.675294127953325
20-21	24.35876348719476	25.65191347607977	27.20411397083382	22.785209065891646
22-23	23.93855449710697	25.47267708937006	26.9220172053015	23.66675120822147
24-25	23.529827890409134	25.810218645585298	27.14219321899075	23.517760245014816
26-27	23.339705862062093	25.969868721819957	27.219115045643267	23.471310370474686
28-29	23.36509410511169	25.92925170366006	27.287032910233567	23.418621280994678
30-31	23.793686934612616	25.89066855662065	27.028298498257513	23.287346010509218
32-33	23.495558553507603	25.880996390777213	26.976093279136588	23.647351776578596
34-35	23.60724069267179	25.958985293821335	27.046245574874312	23.38752843863256
36-37	23.77972057149002	25.814382917938712	26.987907317394754	23.417989193176513
38-39	23.680213246656248	25.851951712861716	26.95073270683732	23.51710233364472
40-41	23.739616028784372	25.883375374057316	26.951619328964778	23.425389268193538
42-43	23.841110368396937	25.775016886283257	26.81973246520462	23.564140280115186
44-45	23.782428244467077	25.840380213979874	26.723742567101418	23.653448974451635
46-47	23.792886541760335	25.830046555146914	26.577324997678375	23.79974190541438
48-49	23.765184358618605	25.980444440550905	26.544903432242688	23.709467768587803
50-51	23.63842644407989	26.2138856826211	26.436304539710143	23.711383333588863
52-53	23.81036233002947	26.069145714995955	26.332425509733643	23.788066445240936
54-55	23.761193729102448	26.080211558906512	26.317063266608642	23.8415314453824
56-57	23.79824650355279	26.15283597858799	26.135248887994322	23.913668629864894
58-59	23.60985515384634	26.04280711175626	26.497032315035185	23.850305419362215
60-61	23.753102037231674	26.208691245263033	26.343121071601512	23.695085645903777
62-63	23.516417280185593	26.256609555782656	26.373830473846727	23.853142690185024
64-65	23.56253976806661	26.183985009423864	26.37879240877575	23.87468281373377
66-67	23.568316481236657	26.24568403216749	26.2461658961224	23.939833590473462
68-69	23.641010455602128	25.99423275136658	26.294334683521903	24.07042210950939
70-71	23.56412338426849	25.8847136920808	26.43827511906543	24.11288780458528
72-73	23.659639194204185	25.94557202644513	26.32443607392055	24.070352705430135
74-75	23.524261145023722	26.043674266050864	26.266649204202203	24.165415384723204
76-77	23.53328670183155	25.867256144912137	26.36371958203341	24.235737571222906
78-79	23.53777002208445	25.983266274034694	26.266724308754853	24.212239395126005
80-81	23.529160545286825	25.98829075305894	26.23759188640121	24.244956815253033
82-83	23.57128355671075	25.95989653176418	26.30831668323464	24.160503228290427
84-85	23.591414893176708	25.908220813323833	26.146068225263246	24.354296068236213
86-87	23.383771082186996	25.905738822321084	26.266310875961242	24.444179219530675
88-89	23.412675383627217	25.870351070560343	26.269751291349518	24.447222254462922
90-91	23.415108247993878	25.915461928040234	26.280464115819598	24.388965708146294
92-93	23.386877414816468	25.89386261569547	26.30290038593262	24.41635958355544
94-95	23.208179690754115	25.900470705822222	26.332436479679323	24.558913123744343
96-97	23.354761253490626	25.81354446328108	26.349881224074228	24.481813059154067
98-99	23.309224050766804	25.77155069617771	26.1737589654273	24.745466287628187
100-101	23.206078083212958	25.863717471532798	26.279173459170952	24.651030986083285
102-103	23.155396382337496	25.776951252788315	26.303868233307213	24.763784131566972
104-105	23.06853554583118	25.83937724662101	26.145883474198595	24.94620373334922
106-107	22.989134778890616	25.798762921817055	26.282359974640883	24.92974232465145
108-109	22.970912409182713	25.809638955783804	26.315809067395694	24.903639567637786
110-111	22.862924629612436	26.01395621394448	26.12988572599817	24.99323343044492
112-113	22.763194170265034	26.092872751336483	26.088449384308916	25.05548369408957
114-115	22.745558144571312	26.07124267719849	26.152066391502537	25.031132786727657
116-117	22.676136279032693	26.009668148976033	26.175344030369097	25.138851541622177
118-119	22.610169016477727	25.988111827474793	26.098821693177765	25.302897462869716
120-121	22.52046703083154	26.12035220656806	26.096572811042044	25.26260795155835
122-123	22.461400206140013	26.1021122234633	26.039989936058056	25.396497634338626
124-125	22.355900255355543	26.21764897638706	25.996316009933807	25.430134758323593
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	32.5
2	81.5
3	119.5
4	94.0
5	81.0
6	112.0
7	287.5
8	488.0
9	515.0
10	451.0
11	377.0
12	360.5
13	404.0
14	487.0
15	564.0
16	637.0
17	743.0
18	870.0
19	986.0
20	1144.5
21	1399.5
22	1705.5
23	2113.0
24	2655.0
25	3347.5
26	4515.5
27	6200.5
28	8141.0
29	11109.0
30	14278.5
31	17639.0
32	22336.0
33	27438.5
34	32595.5
35	37954.5
36	44109.0
37	49694.5
38	53991.0
39	58144.5
40	62861.0
41	67107.0
42	71200.0
43	76579.0
44	81363.5
45	85464.5
46	89309.5
47	92412.5
48	95324.5
49	97595.0
50	99541.5
51	98880.5
52	94132.0
53	91251.0
54	89512.5
55	84850.0
56	78272.5
57	71452.0
58	65898.0
59	59794.5
60	51208.5
61	42028.5
62	33828.5
63	25584.5
64	18484.0
65	13629.5
66	9967.5
67	7188.5
68	5206.0
69	3821.0
70	2913.0
71	2243.5
72	1717.0
73	1425.5
74	1082.5
75	784.0
76	578.5
77	338.0
78	197.0
79	130.0
80	73.0
81	39.0
82	24.0
83	19.5
84	13.0
85	5.0
86	3.5
87	6.0
88	4.5
89	1.5
90	2.5
91	2.0
92	1.5
93	1.0
94	0.0
95	1.5
96	4.5
97	3.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07151064821140177
2	1.3137289934733943E-4
3	4.817006309402446E-4
4	0.1822142113947598
5	0.0662995232039573
6	0.008801984256271742
7	0.013400035733428621
8	0.010641204847134496
9	0.017647759478992598
10-11	0.017538282062869816
12-13	0.01878632460666954
14-15	0.02642784825203978
16-17	0.026843862433306356
18-19	0.02434777734570691
20-21	0.027303667581022043
22-23	0.029558902353151374
24-25	0.026800071466857242
26-27	0.026252684386243327
28-29	0.027303667581022043
30-31	0.027500726930043056
32-33	0.023471958016724644
34-35	0.025836670204976753
36-37	0.029186679138333914
38-39	0.033872312548389016
40-41	0.02936184300413036
42-43	0.028814455923516454
44-45	0.02850791915837266
46-47	0.0303033487827863
48-49	0.025989938587548653
50-51	0.03157328680981058
52-53	0.02817948691000431
54-55	0.031222959078217673
56-57	0.028529814641597213
58-59	0.02485137345987171
60-61	0.02629647535269244
62-63	0.030960213279522992
64-65	0.035404996374107975
66-67	0.033894208031613575
68-69	0.029996812017642503
70-71	0.03952134722032461
72-73	0.04217070069049596
74-75	0.02557392440628208
76-77	0.018698742673771314
78-79	0.022858884486437064
80-81	0.026011834070773208
82-83	0.024369672828931467
84-85	0.01705658143192957
86-87	0.01745070012997159
88-89	0.01762586399576804
90-91	0.01922423427116067
92-93	0.02342816705027553
94-95	0.017910505277687276
96-97	0.021698423875535562
98-99	0.01885201105634321
100-101	0.018479787841525746
102-103	0.017034685948705015
104-105	0.018611160740873087
106-107	0.01635592596874376
108-109	0.01539252470686327
110-111	0.01278696220314104
112-113	0.010838264196155504
114-115	0.00926178940398743
116-117	0.008845775222720856
118-119	0.005692825638384709
120-121	0.0037879185978482873
122-123	0.0077291055782684704
124-125	0.055526945457475475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2283576.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.02111046039829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.49550953287873	39.88783437891378
2	14.407333293190074	15.854149531287698
3	5.4611904940682345	9.014422962582163
4	2.624428391560629	5.775958577098511
5	1.4673219119808387	4.036684050003027
6	0.8774044568245354	2.896546052243311
7	0.5744931320499183	2.2126475054081114
8	0.4074782704100857	1.7935925541156295
9	0.278222860320035	1.3777317657248949
>10	1.326859933745325	12.608553976704156
>50	0.059261158461472736	2.1991174075744526
>100	0.01937751759969188	1.6740539869508175
>500	6.394553775002535E-4	0.2277254947530646
>1k	4.7959153312391087E-4	0.4409817566404357
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2352	0.1029963530883141	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	8.75819328982263E-5	0.0	0.0	0.0
6	0.0	8.75819328982263E-5	0.0	0.0	0.0
7	0.0	8.75819328982263E-5	0.0	4.379096644911315E-5	0.0
8	0.0	8.75819328982263E-5	0.0	4.379096644911315E-5	0.0
9	0.0	8.75819328982263E-5	0.0	4.379096644911315E-5	0.0
10-11	0.0	8.75819328982263E-5	0.0	4.379096644911315E-5	0.0
12-13	0.0	8.75819328982263E-5	0.0	4.379096644911315E-5	1.751638657964526E-4
14-15	0.0	1.0947741612278286E-4	0.0	4.379096644911315E-5	2.4085031547012227E-4
16-17	0.0	1.3137289934733943E-4	0.0	4.379096644911315E-5	2.6274579869467885E-4
18-19	0.0	1.9705934902100917E-4	0.0	4.379096644911315E-5	3.503277315929052E-4
20-21	0.0	2.1895483224556572E-4	0.0	6.568644967366971E-5	3.7222321481746176E-4
22-23	0.0	2.1895483224556572E-4	0.0	1.0947741612278286E-4	3.941186980420183E-4
24-25	0.0	2.6274579869467885E-4	0.0	4.3790966449113144E-4	3.941186980420183E-4
26-27	0.0	2.6274579869467885E-4	0.0	8.758193289822629E-4	5.254915973893577E-4
28-29	0.0	2.6274579869467885E-4	0.0	0.0025836670204976756	5.254915973893577E-4
30-31	0.0	2.6274579869467885E-4	0.0	0.00913041650464009	5.473870806139143E-4
32-33	0.0	2.6274579869467885E-4	0.0	0.024041240580563117	5.911780470630274E-4
34-35	0.0	2.6274579869467885E-4	0.0	0.045148486409035654	6.568644967366972E-4
36-37	0.0	2.6274579869467885E-4	0.0	0.07634955000402877	6.568644967366972E-4
38-39	0.0	2.6274579869467885E-4	0.0	0.12885491877651542	7.006554631858104E-4
40-41	0.0	2.6274579869467885E-4	0.0	0.18694363577126402	7.225509464103669E-4
42-43	0.0	2.6274579869467885E-4	0.0	0.24494477083311436	7.444464296349235E-4
44-45	0.0	2.8464128191923543E-4	0.0	0.3134119468763028	7.663419128594801E-4
46-47	0.0	3.284322483683486E-4	0.0	0.39506020382067425	7.882373960840366E-4
48-49	0.0	3.503277315929052E-4	0.0	0.47800029427529456	8.101328793085931E-4
50-51	0.0	3.503277315929052E-4	0.0	0.5624292775891847	8.758193289822629E-4
52-53	0.0	3.503277315929052E-4	0.0	0.6522883407427649	9.634012618804892E-4
54-55	0.0	3.503277315929052E-4	0.0	0.7531170409918478	9.634012618804892E-4
56-57	0.0	3.503277315929052E-4	0.0	0.8658787795983143	0.0010071922283296023
58-59	0.0	3.7222321481746176E-4	0.0	0.9927193139181705	0.0010071922283296023
60-61	0.0	4.8170063094024455E-4	0.0	1.127245162849846	0.0010071922283296023
62-63	0.0	5.692825638384709E-4	0.0	1.2616615343653987	0.0010509831947787154
64-65	0.0	5.692825638384709E-4	0.0	1.4047923082043252	0.0010509831947787154
66-67	0.0	5.692825638384709E-4	0.0	1.5792117275711428	0.0010509831947787154
68-69	0.0	6.568644967366972E-4	0.0	1.7704687735376443	0.0010509831947787154
70-71	0.0	6.568644967366972E-4	0.0	1.9808405763591839	0.0010509831947787154
72-73	0.0	6.787599799612538E-4	0.0	2.1934238229864036	0.0011385651276769417
74-75	0.0	7.444464296349235E-4	0.0	2.4238737839248614	0.001226147060575168
76-77	0.0	7.882373960840366E-4	0.0	2.6834228420687554	0.001226147060575168
78-79	0.0	7.882373960840366E-4	0.0	2.972027206451635	0.001226147060575168
80-81	0.0	7.882373960840366E-4	0.0	3.2779071070986907	0.001226147060575168
82-83	0.0	7.882373960840366E-4	0.0	3.6006903207951035	0.0012699380270242812
84-85	0.0	8.539238457577063E-4	0.0	3.9287284504654103	0.0012918335102488378
86-87	0.0	8.758193289822629E-4	0.0	4.291799353295008	0.0013794154431470641
88-89	0.0	8.758193289822629E-4	0.0	4.691479504076064	0.0014451018928207339
90-91	0.0	8.758193289822629E-4	0.0	5.112026050370121	0.0014669973760452904
92-93	0.0	8.758193289822629E-4	0.0	5.549454014230312	0.0016640567250662994
94-95	0.0	8.758193289822629E-4	0.0	5.995837230729347	0.001685952208290856
96-97	0.0	8.758193289822629E-4	0.0	6.4682760722656045	0.001795429624413639
98-99	0.0	9.19610295431376E-4	0.0	6.983498688022645	0.001839220590862752
100-101	0.0	9.19610295431376E-4	0.0	7.550657389988334	0.001839220590862752
102-103	0.0	9.19610295431376E-4	0.0	8.12410009563947	0.001839220590862752
104-105	0.0	9.634012618804892E-4	0.0	8.694827761370762	0.001839220590862752
106-107	0.0	9.634012618804892E-4	0.0	9.291939484387644	0.001839220590862752
108-109	0.0	9.852967451050457E-4	0.0	9.918062722677064	0.001839220590862752
110-111	0.0	0.0010071922283296023	0.0	10.584758291381586	0.001839220590862752
112-113	0.0	0.0010290877115541588	0.0	11.27860425928456	0.001839220590862752
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7615	0.0	40.719204	1
GGTATCA	5115	0.0	36.13948	1
TCAACGC	9530	0.0	32.030716	4
ATCAACG	9695	0.0	31.48489	3
CAACGCA	9755	0.0	31.361843	5
TATCAAC	9910	0.0	31.099207	2
AACGCAG	10155	0.0	30.169266	6
ACGCAGA	11315	0.0	26.392868	7
CGCAGAG	11880	0.0	24.987974	8
CATGGGG	7150	0.0	23.6811	4
GTACATG	11630	0.0	23.329077	1
TACATGG	11590	0.0	22.990929	2
GCAGAGT	12675	0.0	22.9059	9
ACATGGG	11675	0.0	22.213266	3
GAGTACT	4795	0.0	21.403065	12-13
AGAGTAC	11605	0.0	20.762774	10-11
GTACTTT	4985	0.0	20.229704	14-15
TACCGTA	265	9.2636037E-7	20.203846	7
AGTACTT	5315	0.0	20.148592	12-13
CAGAGTA	12080	0.0	18.961351	10-11
>>END_MODULE