FastQCFastQC Report
Mon 15 Aug 2016
SRR2921139_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921139_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792334
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29590.1650919973620988No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28290.15783888493997214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG38450.022.906244
GTATAGG3950.022.609381
GTATCAA81950.022.1587581
TACCGAA1653.2163074E-421.6363057
GAGTACT24350.021.62700712-13
GTGTAGC14350.021.159861
CGCATAG1703.9304444E-421.0034565
GTACATG97100.020.9088291
AACGCAG84050.020.1754326
TACATGG98250.019.6246032
CAACGCA87850.019.4414465
TCAACGC87550.019.372124
TATCAAC90500.019.3324642
ATCAACG89800.019.0192783
ACATGGG99000.018.9349373
ACGCAGA92550.018.3224777
GTATAGT5201.8189894E-1218.3193971
GTACTTT29000.018.15998614-15
CGCAGAG93400.018.0920268
GTAGCCC11600.017.9555423
Produced by FastQC (version 0.11.5)