##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921139_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1792334
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.262792537551594	31.0	30.0	34.0	23.0	34.0
2	29.35347429664337	31.0	30.0	34.0	23.0	34.0
3	29.413415133563277	31.0	30.0	34.0	23.0	34.0
4	32.84961229324445	35.0	33.0	37.0	25.0	37.0
5	32.94362211507453	35.0	35.0	37.0	26.0	37.0
6	32.93455795627378	37.0	35.0	37.0	26.0	37.0
7	32.87960000758787	37.0	35.0	37.0	25.0	37.0
8	32.81786486224108	37.0	33.0	37.0	25.0	37.0
9	34.137692528289925	38.0	35.0	39.0	25.0	39.0
10-11	34.091679898947405	38.0	34.0	39.0	25.0	39.0
12-13	34.05759668677824	38.0	34.0	39.0	25.0	39.0
14-15	35.06193962732392	39.0	34.0	41.0	24.0	41.0
16-17	34.97424391882316	39.0	34.0	41.0	24.0	41.0
18-19	35.055653131614974	39.0	34.0	41.0	24.0	41.0
20-21	35.08611955137826	39.0	34.0	41.0	24.0	41.0
22-23	35.058648946011175	39.0	34.0	41.0	24.0	41.0
24-25	34.96326828593331	39.0	34.0	41.0	24.0	41.0
26-27	34.87973781672389	39.0	34.0	41.0	23.5	41.0
28-29	34.725540273185686	39.0	34.0	41.0	23.0	41.0
30-31	34.60958225420039	39.0	34.0	41.0	22.5	41.0
32-33	34.488811237191285	39.0	34.0	41.0	22.0	41.0
34-35	34.37194434742632	39.0	34.0	41.0	20.0	41.0
36-37	34.227089649585395	39.0	33.0	41.0	18.5	41.0
38-39	34.06363378700621	39.0	33.0	41.0	18.0	41.0
40-41	33.943283171551734	39.0	33.0	41.0	16.0	41.0
42-43	33.8544183171217	38.0	33.0	41.0	15.5	41.0
44-45	33.692097566636576	38.0	33.0	40.5	14.5	41.0
46-47	33.45444403777421	38.0	33.0	40.0	10.5	41.0
48-49	33.31334784699727	38.0	33.0	40.0	9.0	41.0
50-51	33.15611738660317	38.0	32.0	40.0	8.0	41.0
52-53	32.95954213890938	38.0	32.0	40.0	7.0	41.0
54-55	32.72423527088144	38.0	32.0	40.0	4.0	41.0
56-57	32.462428598687524	37.5	31.0	40.0	2.0	41.0
58-59	32.2471584537257	37.0	31.0	40.0	2.0	41.0
60-61	31.959211564362448	37.0	31.0	40.0	2.0	41.0
62-63	31.68266991531712	36.0	30.5	40.0	2.0	41.0
64-65	31.37354421664712	36.0	30.0	39.0	2.0	41.0
66-67	31.092868293521185	35.5	30.0	39.0	2.0	40.5
68-69	30.74176939119606	35.0	29.5	39.0	2.0	40.0
70-71	30.420079628015763	35.0	29.0	38.0	2.0	40.0
72-73	30.042225388794723	35.0	29.0	37.0	2.0	39.5
74-75	29.971953051161222	35.0	29.0	37.0	2.0	39.0
76-77	29.702444131506738	35.0	29.0	36.5	2.0	39.0
78-79	29.420753888505157	34.5	29.0	36.0	2.0	39.0
80-81	29.062175632443505	34.0	29.0	36.0	2.0	37.5
82-83	28.791515141709077	34.0	29.0	35.0	2.0	37.0
84-85	28.581983882468336	34.0	29.0	35.0	2.0	36.5
86-87	28.34417441168889	34.0	29.0	35.0	2.0	36.0
88-89	28.166347622708713	34.0	28.0	35.0	2.0	36.0
90-91	27.986355779670532	34.0	27.5	35.0	2.0	35.5
92-93	28.134258179558053	34.0	29.0	35.0	2.0	35.0
94-95	28.10049354640374	34.0	29.0	35.0	2.0	35.0
96-97	28.02949506063044	34.0	29.0	35.0	2.0	35.0
98-99	27.907961350953563	34.0	28.5	35.0	2.0	35.0
100-101	27.782882264131572	34.0	28.0	35.0	2.0	35.0
102-103	26.473635215311432	32.0	24.5	34.0	2.0	35.0
104-105	27.454712123967965	34.0	27.0	35.0	2.0	35.0
106-107	27.605773812247048	34.0	27.0	35.0	2.0	35.0
108-109	27.537835024052434	34.0	27.0	35.0	2.0	35.0
110-111	27.497639948804185	34.0	27.0	35.0	2.0	35.0
112-113	27.400205821013273	34.0	27.0	35.0	2.0	35.0
114-115	27.241835227139585	34.0	27.0	35.0	2.0	35.0
116-117	27.050848502567042	34.0	25.5	35.0	2.0	35.0
118-119	26.910807639647523	34.0	25.0	35.0	2.0	35.0
120-121	26.700270150541137	34.0	25.0	35.0	2.0	35.0
122-123	26.512660865664547	34.0	25.0	35.0	2.0	35.0
124-125	25.425135326339845	33.0	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78643.0
3	11238.0
4	7606.0
5	7808.0
6	10413.0
7	10346.0
8	9647.0
9	10351.0
10	10856.0
11	11667.0
12	12349.0
13	12390.0
14	13170.0
15	11912.0
16	9406.0
17	8630.0
18	8018.0
19	7766.0
20	7833.0
21	8110.0
22	9798.0
23	12700.0
24	16891.0
25	21397.0
26	25187.0
27	28619.0
28	33897.0
29	39713.0
30	46519.0
31	55905.0
32	68934.0
33	87336.0
34	114111.0
35	159371.0
36	260182.0
37	400617.0
38	142882.0
39	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.43315682390225	18.99713460649415	13.143513110906138	24.426195458697464
2	15.544521086821392	20.609576589616342	39.47963409557841	24.366268227983852
3	19.291115364654544	24.478869623933434	29.276885215712316	26.953129795699702
4	12.896621227982848	16.850054575527395	36.577991665234386	33.67533253125537
5	13.623907653969106	37.9634501757407	34.36599709061122	14.046645079678974
6	32.388169465498926	36.3585384471168	17.266105561060968	13.987186526323308
7	27.733498793642575	33.0356126532498	20.888508469053193	18.342380084054433
8	25.164267014246143	36.32594280561811	19.927139990759983	18.582650189375762
9	25.738128237575854	17.050544682731516	19.72100772442915	37.49031935526348
10-11	24.42141438456201	27.15042626163372	27.622791724244138	20.805367629560127
12-13	24.95497860594514	24.81199819105364	27.465045124618943	22.76797807838228
14-15	22.660530059360156	26.220521142922188	25.137798861787118	25.981149935930535
16-17	22.329156077518007	29.027403493784337	26.18820501131199	22.45523541738567
18-19	22.382968086504256	28.18538635129145	27.618747647839808	21.81289791436449
20-21	23.45173951569883	27.403677680139815	27.218839489584333	21.925743314577023
22-23	22.994697758864362	27.28843622664333	26.829713975810442	22.887152038681865
24-25	22.68571924351736	27.36942037423522	27.090723292854836	22.854137089392577
26-27	22.671588148828434	27.418785640736083	27.0767908000905	22.832835410344988
28-29	22.64061929375581	27.53563346790944	27.342822571847936	22.48092466648681
30-31	22.95265363763605	27.476323471030618	26.983863943687986	22.587158947645346
32-33	22.564341478860175	27.545810742387193	27.0575812820731	22.83226649667953
34-35	22.549894585073627	27.666417346021255	27.094244598897394	22.68944347000773
36-37	22.83564122200229	27.44062190938904	26.928464518216906	22.795272350391762
38-39	22.738838524154104	27.613317863578075	27.01317207443225	22.634671537835573
40-41	22.783566067590762	27.578786330089113	27.018143006032417	22.619504596287708
42-43	22.84763242216639	27.49245808637646	26.89937103760105	22.760538453856096
44-45	22.92552164641529	27.50314399438489	26.78761327585803	22.783721083341796
46-47	22.730644518298377	27.422721884709194	26.952872506000052	22.893761090992378
48-49	22.65083076284447	27.39420916772513	27.112085403184793	22.842874666245606
50-51	22.649722390692904	27.50298923095722	26.851049536704075	22.996238841645805
52-53	22.68270861446124	27.59073114133305	26.508577580422926	23.21798266378278
54-55	22.80506030220428	27.298175018252046	26.56566594941762	23.33109873012605
56-57	22.740421082642857	27.35840398011838	26.619747660628732	23.281427276610025
58-59	22.654154224525413	27.122500970821783	26.81797537906685	23.405369425585953
60-61	22.930968345787367	27.123920530392535	26.691142316324523	23.253968807495575
62-63	22.644782894703805	27.082799797130708	26.81476236785738	23.45765494030811
64-65	22.435070704771277	26.944475364152797	26.97424197661466	23.64621195446127
66-67	22.664311789987106	26.904094104309024	26.72544187414112	23.70615223156275
68-69	22.714630205346005	26.83937661943478	26.875001394861997	23.570991780357218
70-71	22.652300832223034	26.7060404796497	26.87292027932699	23.768738408800274
72-73	22.71494341182782	26.657521948857095	26.980683666691498	23.646850972623593
74-75	22.723400076661743	26.701051002377408	26.916780720073604	23.658768200887245
76-77	22.550394772300827	26.611268543920406	27.15383984480268	23.684496838976084
78-79	22.597556862547087	26.599450490744854	27.22179803735536	23.581194609352703
80-81	22.609976229272448	26.63707948952495	26.952199943035062	23.800744338167544
82-83	22.565638593487204	26.531585779097473	27.08262720079717	23.820148426618147
84-85	22.696744875029815	26.526820572691097	26.905183344003813	23.871251208275275
86-87	22.60913302286241	26.383407975424184	27.001625257834625	24.00583374387878
88-89	22.667823817386665	26.232394808220683	27.122909882563707	23.97687149182895
90-91	22.66580335308614	26.302181698681988	27.120479111140373	23.9115358370915
92-93	22.445847683052197	26.31256512296471	27.24235716188715	23.999230032095944
94-95	22.276853737995246	26.410678727073595	27.209675106608174	24.102792428322985
96-97	22.530783520414527	26.274155568121554	27.176843126582973	24.018217784880946
98-99	22.323200030351924	26.337883890492037	27.044068318614883	24.294847760541156
100-101	22.15131731096216	26.47379228943264	27.09101373007351	24.28387666953169
102-103	22.170264655330417	26.39916486007026	27.139453247814437	24.291117236784878
104-105	22.075461960046923	26.576843620578305	26.937372627079988	24.410321792294784
106-107	21.79704361372802	26.689526991587936	27.106531234446386	24.406898160237652
108-109	21.798335397508872	26.68985946214998	27.120699723421872	24.391105416919284
110-111	21.75388817512098	26.83582268610161	27.00601099528076	24.404278143496644
112-113	21.69688360295537	26.900893954120548	26.900252306861383	24.501970136062695
114-115	21.75549497149846	26.914284470790946	26.871933908571886	24.458286649138707
116-117	21.636564040717843	26.734691543283702	26.982866289996764	24.645878126001687
118-119	21.67385620021598	26.58161601446629	27.021580317407	24.722947467910725
120-121	21.715790768736333	26.64647413176703	26.992213403680797	24.645521695815837
122-123	21.65647082978927	26.53236746969634	27.000699932182737	24.810461768331653
124-125	21.58503585970602	26.579625075661635	26.885342749996095	24.949996314636255
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1181.0
1	790.5
2	329.5
3	236.0
4	182.0
5	147.0
6	153.5
7	188.5
8	216.5
9	245.5
10	269.5
11	268.5
12	292.0
13	325.0
14	359.0
15	431.5
16	533.5
17	606.0
18	741.5
19	879.5
20	1016.0
21	1326.5
22	1706.5
23	2183.0
24	2826.5
25	3784.0
26	5367.0
27	7319.0
28	9617.5
29	12958.0
30	16651.0
31	20305.0
32	25066.0
33	30192.5
34	34023.5
35	37916.0
36	43094.5
37	47472.5
38	50083.5
39	52077.5
40	54952.5
41	57254.0
42	58718.0
43	61253.5
44	65119.0
45	68117.5
46	70205.0
47	72012.0
48	72127.0
49	72882.0
50	77449.0
51	77364.0
52	70642.5
53	65705.5
54	62404.5
55	58358.0
56	52936.0
57	47249.0
58	42897.5
59	38084.0
60	31608.5
61	25580.5
62	19860.5
63	14865.0
64	11002.0
65	8133.5
66	6000.0
67	4401.5
68	3195.5
69	2324.5
70	1737.5
71	1305.5
72	1003.0
73	809.0
74	613.0
75	407.5
76	287.5
77	188.0
78	132.5
79	97.0
80	51.5
81	32.5
82	25.0
83	18.5
84	17.0
85	18.5
86	15.5
87	12.0
88	8.5
89	8.0
90	8.0
91	5.0
92	5.0
93	6.0
94	6.5
95	5.5
96	4.5
97	4.0
98	5.0
99	7.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07286588325613418
2	0.009596425666198376
3	0.020308714781954704
4	0.017965401537883004
5	0.011437600357968994
6	0.00881532125150781
7	0.0090384939414194
8	0.007253112422126679
9	0.005467730902833958
10-11	0.010238047149694198
12-13	0.007281009008365629
14-15	0.007727354388188808
16-17	0.011632876461641635
18-19	0.005495627489072907
20-21	0.012609256980004844
22-23	0.00407290159088652
24-25	0.0036544527973022887
26-27	0.0030965210725233134
28-29	0.009122183700136247
30-31	0.0058861796964181905
32-33	0.0023154166578327477
34-35	0.007281009008365629
36-37	0.006220938731285575
38-39	0.001087966863319002
40-41	0.0010042771046021557
42-43	9.484839321242581E-4
44-45	0.0015622088293811309
46-47	0.0032081074174791085
48-49	0.0019806576229653627
50-51	0.0037381425560191347
52-53	0.0041565913496033665
54-55	0.0038776254872138786
56-57	0.0014227258981863871
58-59	0.002120140554160106
60-61	0.004268177694559162
62-63	0.002622279106461184
64-65	0.0032081074174791085
66-67	0.0013948293119474384
68-69	0.0023433132440716965
70-71	0.001450622484425336
72-73	0.0023712098303106453
74-75	0.0025106927615053893
76-77	0.0019527610367264137
78-79	4.184487935842315E-4
80-81	1.9527610367264136E-4
82-83	2.7896586238948765E-5
84-85	3.62655621106334E-4
86-87	1.673795174336926E-4
88-89	2.7896586238948765E-5
90-91	8.36897587168463E-4
92-93	0.002873348382611723
94-95	0.0012832429669916432
96-97	0.001087966863319002
98-99	0.001450622484425336
100-101	0.001534312243142182
102-103	0.0033196937624349033
104-105	0.005941972868896087
106-107	0.00814580318177304
108-109	0.014199362395624922
110-111	0.011242324254296353
112-113	0.004017108418408623
114-115	0.00836897587168463
116-117	0.0027059688651780303
118-119	0.0016458985880979772
120-121	0.004881902591816034
122-123	9.763805183632069E-4
124-125	0.08179279085259779
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1792334.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.29051358143178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.10219239007	34.75184744899527
2	14.101798768776067	14.183734050075014
3	6.230228100663825	9.399641127355562
4	3.443783330041905	6.9275852932392326
5	2.0449096979056627	5.141977946766314
6	1.3256099830296222	3.999936411313966
7	0.8753880646379198	3.081660074758761
8	0.6156663718531992	2.476974242825131
9	0.428400013487244	1.939001102690922
>10	1.766745953429431	14.124229187475976
>50	0.04896953451129951	1.6714029874241545
>100	0.01463269981739276	1.2519151679307445
>500	7.817094956541647E-4	0.28529695114326314
>1k	8.933822807476169E-4	0.7647980080057003
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2959	0.1650919973620988	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2829	0.15783888493997214	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	5.579317247789754E-5	0.0
20-21	0.0	0.0	0.0	1.9527610367264136E-4	0.0
22-23	0.0	0.0	0.0	5.021385523010778E-4	0.0
24-25	0.0	0.0	0.0	0.001339036139469541	0.0
26-27	0.0	0.0	0.0	0.0029291415550896207	0.0
28-29	0.0	0.0	0.0	0.007727354388188809	0.0
30-31	0.0	0.0	0.0	0.022596234853548502	0.0
32-33	0.0	0.0	0.0	0.054760998787056425	0.0
34-35	0.0	0.0	0.0	0.09671746449043538	0.0
36-37	0.0	0.0	0.0	0.1637250646363903	0.0
38-39	0.0	0.0	0.0	0.25941035543598456	0.0
40-41	5.579317247789754E-5	0.0	0.0	0.3693508018036817	0.0
42-43	5.579317247789754E-5	0.0	0.0	0.4660682662941171	0.0
44-45	5.579317247789754E-5	0.0	0.0	0.5813090640472144	0.0
46-47	5.579317247789754E-5	0.0	0.0	0.72419537876311	0.0
48-49	5.579317247789754E-5	0.0	0.0	0.8642083450963938	0.0
50-51	5.579317247789754E-5	0.0	0.0	1.0075689017783516	0.0
52-53	5.579317247789754E-5	0.0	0.0	1.1531332887731862	0.0
54-55	5.579317247789754E-5	0.0	0.0	1.3174720783068334	0.0
56-57	5.579317247789754E-5	0.0	0.0	1.5126365956345191	0.0
58-59	5.579317247789754E-5	0.0	0.0	1.7179275737669428	0.0
60-61	5.579317247789754E-5	0.0	0.0	1.9306669404251662	0.0
62-63	5.579317247789754E-5	0.0	0.0	2.148650865296312	0.0
64-65	5.579317247789754E-5	0.0	0.0	2.394029237854105	0.0
66-67	5.579317247789754E-5	0.0	0.0	2.675059447625275	0.0
68-69	5.579317247789754E-5	0.0	0.0	2.9922715297483613	0.0
70-71	5.579317247789754E-5	0.0	0.0	3.336682783454423	0.0
72-73	5.579317247789754E-5	0.0	0.0	3.675347340395261	0.0
74-75	5.579317247789754E-5	0.0	0.0	4.033762680393275	0.0
76-77	5.579317247789754E-5	0.0	0.0	4.44334035955352	0.0
78-79	5.579317247789754E-5	0.0	0.0	4.896910955212588	0.0
80-81	5.579317247789754E-5	0.0	0.0	5.379968242526226	0.0
82-83	5.579317247789754E-5	0.0	0.0	5.872873024782211	0.0
84-85	5.579317247789754E-5	0.0	0.0	6.380590894331078	0.0
86-87	5.579317247789754E-5	0.0	0.0	6.9276987436493425	2.789658623894877E-5
88-89	5.579317247789754E-5	0.0	0.0	7.530990317652849	5.579317247789754E-5
90-91	5.579317247789754E-5	0.0	0.0	8.163350134517339	5.579317247789754E-5
92-93	5.579317247789754E-5	0.0	0.0	8.818055116959227	5.579317247789754E-5
94-95	5.579317247789754E-5	0.0	0.0	9.488465877453645	5.579317247789754E-5
96-97	5.579317247789754E-5	0.0	0.0	10.195560648852279	5.579317247789754E-5
98-99	5.579317247789754E-5	0.0	0.0	10.955742623863632	5.579317247789754E-5
100-101	5.579317247789754E-5	0.0	0.0	11.781481576536516	5.579317247789754E-5
102-103	5.579317247789754E-5	0.0	0.0	12.607053149691966	5.579317247789754E-5
104-105	5.579317247789754E-5	0.0	0.0	13.432317860398786	5.579317247789754E-5
106-107	1.1158634495579507E-4	0.0	0.0	14.282047877237167	5.579317247789754E-5
108-109	1.1158634495579507E-4	0.0	0.0	15.177277226231272	5.579317247789754E-5
110-111	1.1158634495579507E-4	0.0	0.0	16.10037526487809	5.579317247789754E-5
112-113	1.1158634495579507E-4	0.0	0.0	17.062946973053016	5.579317247789754E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	3845	0.0	22.90624	4
GTATAGG	395	0.0	22.60938	1
GTATCAA	8195	0.0	22.158758	1
TACCGAA	165	3.2163074E-4	21.636305	7
GAGTACT	2435	0.0	21.627007	12-13
GTGTAGC	1435	0.0	21.15986	1
CGCATAG	170	3.9304444E-4	21.003456	5
GTACATG	9710	0.0	20.908829	1
AACGCAG	8405	0.0	20.175432	6
TACATGG	9825	0.0	19.624603	2
CAACGCA	8785	0.0	19.441446	5
TCAACGC	8755	0.0	19.37212	4
TATCAAC	9050	0.0	19.332464	2
ATCAACG	8980	0.0	19.019278	3
ACATGGG	9900	0.0	18.934937	3
ACGCAGA	9255	0.0	18.322477	7
GTATAGT	520	1.8189894E-12	18.319397	1
GTACTTT	2900	0.0	18.159986	14-15
CGCAGAG	9340	0.0	18.092026	8
GTAGCCC	1160	0.0	17.955542	3
>>END_MODULE