FastQCFastQC Report
Mon 15 Aug 2016
SRR2921139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792334
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32440.1809930515182996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27910.155718744385812No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27430.15304067210687294No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26690.14891197734350853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94050.027.0176541
GGTATCA71750.025.521921
GAGTACT51200.024.11241312-13
GTACCGT1501.6883908E-423.797336
TCAACGC108400.022.830024
CAACGCA108950.022.731995
ATCAACG109100.022.6829073
TATCAAC110450.022.6737042
AACGCAG109900.022.6280356
TACCGTA1350.00255791422.033957
AGTACTT55900.021.6061212-13
GTACTTT58050.021.16461614-15
ACGCAGA122200.020.1064347
CATGGGG38600.019.9292014
CGCAGAG122700.019.8784878
ACTGCGC2701.1078537E-619.8300048
CAGTGCG3101.9555773E-719.1919339
AGTCCGT1959.922463E-418.3046198
GCAGAGT126550.018.2880699
ACTTTTT72800.018.10259816-17
Produced by FastQC (version 0.11.5)