##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921139_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1792334
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.448569853609875	31.0	30.0	34.0	27.0	34.0
2	30.518306855753448	31.0	30.0	34.0	27.0	34.0
3	30.61559620026178	31.0	30.0	34.0	27.0	34.0
4	34.12627947692785	37.0	35.0	37.0	30.0	37.0
5	33.760972564265366	37.0	35.0	37.0	28.0	37.0
6	33.60499214990063	37.0	35.0	37.0	28.0	37.0
7	33.42419995380325	37.0	35.0	37.0	27.0	37.0
8	33.40530559594362	37.0	35.0	37.0	27.0	37.0
9	34.78507075132202	39.0	35.0	39.0	27.0	39.0
10-11	34.74575888199409	38.5	35.0	39.0	27.0	39.0
12-13	34.609940446367695	38.0	35.0	39.0	27.0	39.0
14-15	35.65626663333954	39.0	35.0	41.0	27.0	41.0
16-17	35.60283490688677	39.0	35.0	41.0	27.0	41.0
18-19	35.66731619218293	39.0	35.0	41.0	27.0	41.0
20-21	35.68276643750551	39.0	35.0	41.0	27.0	41.0
22-23	35.654475672503004	39.0	35.0	41.0	27.0	41.0
24-25	35.56506209222165	39.0	35.0	41.0	27.0	41.0
26-27	35.45123704622018	39.0	35.0	41.0	26.0	41.0
28-29	35.34771197778986	39.0	35.0	41.0	25.0	41.0
30-31	35.236894462750804	39.0	35.0	41.0	25.0	41.0
32-33	35.06832682970919	39.0	34.0	41.0	25.0	41.0
34-35	34.9297343575472	39.0	34.0	41.0	24.0	41.0
36-37	34.778546297732454	39.0	34.0	41.0	24.0	41.0
38-39	34.59455408422761	39.0	34.0	41.0	23.0	41.0
40-41	34.4445220589466	39.0	34.0	41.0	22.5	41.0
42-43	34.29850797898159	39.0	33.0	41.0	20.5	41.0
44-45	34.10106542642164	38.5	33.0	41.0	18.5	41.0
46-47	33.848019956101936	38.0	33.0	41.0	15.5	41.0
48-49	33.618563839105875	38.0	33.0	40.5	14.0	41.0
50-51	33.40577537445587	38.0	33.0	40.0	10.0	41.0
52-53	33.23839167253425	38.0	33.0	40.0	8.5	41.0
54-55	33.05325486209602	38.0	32.0	40.0	8.0	41.0
56-57	32.79631363350804	38.0	32.0	40.0	7.0	41.0
58-59	32.58565535218324	37.0	32.0	40.0	4.0	41.0
60-61	32.33505167005703	37.0	31.0	40.0	2.0	41.0
62-63	32.05149151887985	36.5	31.0	40.0	2.0	41.0
64-65	31.770842097510844	36.0	31.0	39.5	2.0	41.0
66-67	31.450498902548297	36.0	31.0	39.0	2.0	41.0
68-69	31.094992060631554	35.0	30.0	39.0	2.0	40.5
70-71	30.728418363987963	35.0	30.0	38.0	2.0	40.0
72-73	30.526374548493752	35.0	30.0	37.5	2.0	40.0
74-75	30.31658915135237	35.0	30.0	37.0	2.0	39.0
76-77	30.0628869953926	35.0	30.0	36.5	2.0	39.0
78-79	29.71953246437327	35.0	29.0	36.0	2.0	39.0
80-81	29.40887580105047	34.5	29.0	36.0	2.0	37.5
82-83	29.167592647352556	34.0	29.0	35.0	2.0	37.0
84-85	28.98952929532107	34.0	29.0	35.0	2.0	36.5
86-87	28.761202432135974	34.0	29.0	35.0	2.0	36.0
88-89	28.594648932620817	34.0	29.0	35.0	2.0	36.0
90-91	28.42001295517465	34.0	29.0	35.0	2.0	36.0
92-93	28.282465489133166	34.0	29.0	35.0	2.0	35.0
94-95	28.177698743649344	34.0	29.0	35.0	2.0	35.0
96-97	28.106398137847073	34.0	29.0	35.0	2.0	35.0
98-99	28.05357065145224	34.0	29.0	35.0	2.0	35.0
100-101	27.89360855733362	34.0	28.0	35.0	2.0	35.0
102-103	26.5453422743752	32.0	25.0	34.0	2.0	35.0
104-105	27.608015023985484	34.0	27.0	35.0	2.0	35.0
106-107	27.87006021199174	34.0	28.0	35.0	2.0	35.0
108-109	28.062855751216013	34.0	29.0	35.0	2.0	35.0
110-111	28.032888122414683	34.0	29.0	35.0	2.0	35.0
112-113	27.948213893169466	34.0	29.0	35.0	2.0	35.0
114-115	27.815065997743723	34.0	29.0	35.0	2.0	35.0
116-117	27.706006525569453	34.0	29.0	35.0	2.0	35.0
118-119	27.545416200328734	34.0	28.5	35.0	2.0	35.0
120-121	27.36074777357345	34.0	27.0	35.0	2.0	35.0
122-123	27.156650769332053	34.0	27.0	35.0	2.0	35.0
124-125	26.35885024777748	33.5	25.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48987.0
3	15876.0
4	7747.0
5	6613.0
6	7698.0
7	9318.0
8	10134.0
9	10999.0
10	12047.0
11	13076.0
12	13956.0
13	15620.0
14	17639.0
15	14504.0
16	9923.0
17	9092.0
18	8401.0
19	8186.0
20	7903.0
21	8096.0
22	8662.0
23	10620.0
24	14305.0
25	18779.0
26	23677.0
27	27439.0
28	32326.0
29	38919.0
30	46068.0
31	55723.0
32	67918.0
33	85090.0
34	111512.0
35	155982.0
36	261315.0
37	420343.0
38	157602.0
39	239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.135744302109636	19.53358206871752	13.861990947699681	24.468682681473158
2	15.791112244334332	20.855969126238403	38.78067165049313	24.572246978934135
3	19.044510541383026	24.522782521172314	30.01115316096427	26.421553776480387
4	13.164943117858991	17.29106418845276	36.18664407105545	33.3573486226328
5	13.394268485043062	37.727215622775304	34.75492399391393	14.123591898267703
6	32.274010393097456	36.65120851981781	16.93635190338314	14.138429183701598
7	27.47179531248082	32.75621931634672	21.121067274973523	18.650918096198936
8	25.566582670627646	35.93475538850586	19.861027102672324	18.637634838194167
9	25.91724548178232	16.050798455153757	19.908170821468254	38.12378524159567
10-11	24.754683604698045	26.49263611366487	27.78726271573041	20.965417565906677
12-13	25.52066868359544	23.971364931388056	27.891597007475173	22.616369377541332
14-15	22.91601209673144	25.408353977283195	25.744898260997196	25.930735664988173
16-17	22.33183686327822	28.38061179758617	26.765969997491467	22.521581341644143
18-19	22.27188942406694	27.168461661663674	28.867303188132105	21.692345726137283
20-21	23.232766029640576	26.38489553918034	28.575040125723543	21.807298305455536
22-23	22.760279949135672	26.36872284717457	28.21558237674372	22.65541482694604
24-25	22.475166660109362	26.57308978118423	28.43695405228709	22.51478950641932
26-27	22.409828431051114	26.581230518272886	28.511189358132288	22.49775169254371
28-29	22.42840548150699	26.5542409520283	28.79230997396867	22.225043592496036
30-31	22.716107463639457	26.577497773986604	28.366037802186028	22.340356960187915
32-33	22.493528003834925	26.51141130533145	28.48705126423658	22.508009426597045
34-35	22.398031589879587	26.66154362150183	28.43909519655919	22.501329592059392
36-37	22.669661729928077	26.49994767940731	28.28624247455475	22.54414811610986
38-39	22.57019595148931	26.59705207699649	28.352941504769024	22.479810466745175
40-41	22.67593418865094	26.479770337790743	28.325349959734332	22.518945513823986
42-43	22.87654669963465	26.402308912154243	28.069310895311773	22.651833492899335
44-45	22.915808028565056	26.53005472867408	27.876882946268346	22.677254296492517
46-47	22.849461165400736	26.492496497993674	27.773170445857026	22.884871890748563
48-49	22.867490265869968	26.533421954795237	27.768820759744873	22.830267019589918
50-51	22.757126313292687	26.73951807565124	27.468794910075207	23.034560700980872
52-53	22.79163563519208	26.90807642434817	27.17829790048182	23.121990039977934
54-55	22.784417695023677	26.701508442977772	27.153313024311387	23.36076083768716
56-57	22.814632921282474	26.815497949047074	27.064960794709382	23.304908334961073
58-59	22.52843318898165	26.701164114469396	27.429461136657885	23.340941559891064
60-61	22.899186922451673	26.81026879078607	27.166539566620933	23.124004720141325
62-63	22.61283451737385	26.830063234711815	27.249916495355414	23.307185752558926
64-65	22.405208614719676	26.701573456626132	27.416499416204726	23.476718512449462
66-67	22.58287132860069	26.78169833376913	27.1598227085035	23.47560762912668
68-69	22.623059235860747	26.673354466408455	27.260662343315733	23.442923954415065
70-71	22.615651579853914	26.467569334017245	27.207305209211828	23.709473876917013
72-73	22.704322549100596	26.447063787795233	27.28376091465416	23.56485274845001
74-75	22.720559449769475	26.546323504586045	27.129251100854486	23.603865944789995
76-77	22.515446497529005	26.470012142911354	27.32341997062487	23.69112138893477
78-79	22.515637351289257	26.481387387492966	27.329488904086542	23.67348635713124
80-81	22.4248559679509	26.618897350848314	27.10777060124992	23.848476079950867
82-83	22.385588817320475	26.503615686913545	27.17529970746364	23.93549578830234
84-85	22.52576886687297	26.454678796999946	26.972106873939232	24.047445462187852
86-87	22.420263841995023	26.361766710685902	27.07777454430602	24.140194903013054
88-89	22.354332308049447	26.296383307366415	27.146818346182865	24.202466038401273
90-91	22.357976740552242	26.325392307864014	27.18392151610527	24.132709435478468
92-93	22.194408852259944	26.363521706519993	27.18870542171902	24.253364019501046
94-95	22.01274602617899	26.456388763564032	27.186463018192704	24.344402192064273
96-97	22.31559162722355	26.282327986108804	27.064502924645627	24.33757746202202
98-99	22.21066758930116	26.250299949217897	26.922772144622957	24.616260316857982
100-101	22.03769246973707	26.426888355345486	26.91776378660186	24.617655388315583
102-103	22.08003571403659	26.282154839879745	26.900007672937548	24.737801773146114
104-105	22.012791301780858	26.450907617693982	26.75001492746369	24.786286153061468
106-107	21.809216473629114	26.546495302591666	26.847100418961055	24.79718780481816
108-109	21.760905424197176	26.528171183296838	26.911755514931553	24.799167877574433
110-111	21.63939182996461	26.72064438458287	26.725303729289283	24.91466005616324
112-113	21.538116872174477	26.81743005313183	26.718693677858834	24.925759396834856
114-115	21.639734397232374	26.804257456128116	26.560499958150825	24.995508188488685
116-117	21.518699873811805	26.65829503932256	26.69729815538696	25.12570693147867
118-119	21.52958089351838	26.550003780207838	26.712008702010543	25.20840662426324
120-121	21.4947128324845	26.712080363160783	26.62651819151643	25.16668861283829
122-123	21.367678030737366	26.710183415928114	26.63881795586222	25.283320597472304
124-125	21.160817186449375	26.81401061718271	26.543515013748078	25.481657182619845
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	79.5
2	127.0
3	216.5
4	169.5
5	111.0
6	175.5
7	399.0
8	667.0
9	730.0
10	576.0
11	403.0
12	360.5
13	408.0
14	473.0
15	544.0
16	622.5
17	743.0
18	851.0
19	962.0
20	1168.0
21	1437.5
22	1813.5
23	2374.0
24	3052.0
25	4062.0
26	5737.0
27	7749.5
28	10041.0
29	13308.0
30	16940.0
31	20493.0
32	25182.0
33	30418.0
34	34545.5
35	38513.5
36	43230.5
37	47260.5
38	50030.0
39	51950.5
40	54355.0
41	56363.5
42	58086.0
43	61415.5
44	65241.5
45	67943.5
46	70234.0
47	72666.0
48	73084.0
49	73791.5
50	79158.5
51	78418.5
52	70487.0
53	64876.5
54	61331.0
55	57549.5
56	52485.0
57	47169.0
58	42986.0
59	38219.5
60	31206.5
61	24970.0
62	19329.0
63	14300.5
64	10504.0
65	7716.0
66	5652.0
67	4125.0
68	2951.0
69	2086.5
70	1534.0
71	1144.0
72	853.0
73	699.5
74	529.0
75	344.0
76	226.5
77	132.0
78	88.0
79	48.5
80	18.5
81	12.0
82	6.0
83	1.0
84	1.5
85	2.0
86	1.5
87	2.0
88	2.5
89	2.0
90	1.0
91	1.5
92	1.0
93	0.0
94	1.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07855678684887973
2	1.673795174336926E-4
3	9.484839321242581E-4
4	0.17831497923936054
5	0.07582292139746276
6	0.010210150563455249
7	0.014283052154341768
8	0.01037753008088894
9	0.017351676640626135
10-11	0.01737957322686508
12-13	0.018718609366334623
14-15	0.024827961752664402
16-17	0.02513482420129284
18-19	0.022707821198504296
20-21	0.02599961837470025
22-23	0.028370828205010894
24-25	0.02446530613155807
26-27	0.02401896075173489
28-29	0.025357996891204427
30-31	0.025302203718726532
32-33	0.021368785059034758
34-35	0.024046857337973838
36-37	0.027701310135276123
38-39	0.03146734927753421
40-41	0.027059688651780302
42-43	0.02758972379032033
44-45	0.02758972379032033
46-47	0.028119758928860355
48-49	0.02471637540770861
50-51	0.031188383415144724
52-53	0.026250687650850788
54-55	0.02898455310226777
56-57	0.027003895479302407
58-59	0.02309837340584958
60-61	0.02446530613155807
62-63	0.028872966757311977
64-65	0.034033835211517496
66-67	0.03322483421058798
68-69	0.028677690653639336
70-71	0.03799515045744822
72-73	0.03938997976939566
74-75	0.02401896075173489
76-77	0.01908126498744096
78-79	0.02178723385261899
80-81	0.02558116958111602
82-83	0.023433132440716965
84-85	0.016598468812174517
86-87	0.017546952744298773
88-89	0.017295883468148233
90-91	0.02086664650673368
92-93	0.02399106416549594
94-95	0.01810488446907775
96-97	0.022735717784743245
98-99	0.020308714781954704
100-101	0.018495436676423033
102-103	0.01793750495164406
104-105	0.018690712780095674
106-107	0.015733674638767105
108-109	0.016180020018590285
110-111	0.013195085291022767
112-113	0.010489116425844736
114-115	0.010265943735933146
116-117	0.009456942735003632
118-119	0.005830386523940293
120-121	0.0039055220734528274
122-123	0.008396872457923579
124-125	0.0553747236843133
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1792334.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.98077235414775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.02390016709626	33.31863168725815
2	14.549013159432922	14.252438030793677
3	6.466808504535633	9.502478256555793
4	3.5504712805182885	6.956193021640229
5	2.098381336884982	5.139016928707775
6	1.3426880898310167	3.945953980238311
7	0.9329036682970119	3.198603954364371
8	0.6510016578180388	2.550925120300652
9	0.45998225910411483	2.027725768811273
>10	1.8526855269136229	14.44278051240982
>50	0.05189479969580987	1.7325801635314644
>100	0.01820869441661752	1.5452207728012046
>500	0.0011449196976138134	0.4265372624582098
>1k	9.159357580910506E-4	0.9609145401290329
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3244	0.1809930515182996	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2791	0.155718744385812	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2743	0.15304067210687294	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2669	0.14891197734350853	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.579317247789754E-5	0.0
10-11	0.0	0.0	0.0	5.579317247789754E-5	0.0
12-13	0.0	0.0	0.0	5.579317247789754E-5	0.0
14-15	0.0	0.0	0.0	5.579317247789754E-5	0.0
16-17	0.0	0.0	0.0	5.579317247789754E-5	0.0
18-19	0.0	0.0	0.0	1.1158634495579507E-4	0.0
20-21	0.0	0.0	0.0	2.510692761505389E-4	0.0
22-23	0.0	0.0	0.0	5.579317247789753E-4	0.0
24-25	0.0	0.0	0.0	0.0014785190706642845	0.0
26-27	0.0	0.0	0.0	0.003152314245001211	0.0
28-29	0.0	0.0	0.0	0.007894733905622502	0.0
30-31	0.0	0.0	0.0	0.022345165577397963	0.0
32-33	0.0	0.0	0.0	0.0532545831301532	0.0
34-35	0.0	0.0	0.0	0.09448573759131948	0.0
36-37	2.789658623894877E-5	0.0	0.0	0.16026588794276067	0.0
38-39	5.579317247789754E-5	0.0	0.0	0.2537752450157169	0.0
40-41	5.579317247789754E-5	0.0	0.0	0.3621534825540329	0.0
42-43	5.579317247789754E-5	0.0	0.0	0.4578108767673882	0.0
44-45	5.579317247789754E-5	0.0	0.0	0.5714057759323876	0.0
46-47	5.579317247789754E-5	0.0	0.0	0.7118092944730168	0.0
48-49	5.579317247789754E-5	0.0	0.0	0.8490326021824057	0.0
50-51	5.579317247789754E-5	0.0	0.0	0.9898266729303802	0.0
52-53	5.579317247789754E-5	0.0	0.0	1.1327129876462758	0.0
54-55	5.579317247789754E-5	0.0	0.0	1.2952384990743913	0.0
56-57	5.579317247789754E-5	0.0	0.0	1.4878086338818546	0.0
58-59	5.579317247789754E-5	0.0	0.0	1.6911468509775522	0.0
60-61	5.579317247789754E-5	0.0	0.0	1.9007339033907744	0.0
62-63	5.579317247789754E-5	0.0	0.0	2.1171835160187777	0.0
64-65	5.579317247789754E-5	0.0	0.0	2.359521160676526	0.0
66-67	5.579317247789754E-5	0.0	0.0	2.6383475401348186	0.0
68-69	5.579317247789754E-5	0.0	0.0	2.952742067047771	0.0
70-71	5.579317247789754E-5	0.0	0.0	3.2940567996813095	0.0
72-73	5.579317247789754E-5	0.0	0.0	3.6318007692762624	0.0
74-75	5.579317247789754E-5	0.0	0.0	3.986254794028345	0.0
76-77	5.579317247789754E-5	0.0	0.0	4.3930986077371745	0.0
78-79	5.579317247789754E-5	0.0	0.0	4.844019027703542	0.0
80-81	5.579317247789754E-5	0.0	0.0	5.32439824273824	0.0
82-83	5.579317247789754E-5	0.0	0.0	5.8142344005079405	0.0
84-85	5.579317247789754E-5	0.0	0.0	6.317879368465922	0.0
86-87	5.579317247789754E-5	0.0	0.0	6.861834903539184	0.0
88-89	5.579317247789754E-5	0.0	0.0	7.460607230571981	0.0
90-91	5.579317247789754E-5	0.0	0.0	8.086467142842796	0.0
92-93	5.579317247789754E-5	0.0	0.0	8.73277525282676	0.0
94-95	5.579317247789754E-5	0.0	0.0	9.393254828620112	0.0
96-97	5.579317247789754E-5	0.0	0.0	10.093403350045248	0.0
98-99	5.579317247789754E-5	0.0	0.0	10.845551108219786	0.0
100-101	5.579317247789754E-5	0.0	0.0	11.665348088023773	0.0
102-103	5.579317247789754E-5	0.0	0.0	12.48483820537913	0.0
104-105	5.579317247789754E-5	0.0	0.0	13.306113704253782	0.0
106-107	5.579317247789754E-5	0.0	0.0	14.15408623615911	0.0
108-109	5.579317247789754E-5	0.0	0.0	15.046414340184363	0.0
110-111	5.579317247789754E-5	0.0	0.0	15.967810687070603	0.0
112-113	5.579317247789754E-5	0.0	0.0	16.93068925769416	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9405	0.0	27.017654	1
GGTATCA	7175	0.0	25.52192	1
GAGTACT	5120	0.0	24.112413	12-13
GTACCGT	150	1.6883908E-4	23.79733	6
TCAACGC	10840	0.0	22.83002	4
CAACGCA	10895	0.0	22.73199	5
ATCAACG	10910	0.0	22.682907	3
TATCAAC	11045	0.0	22.673704	2
AACGCAG	10990	0.0	22.628035	6
TACCGTA	135	0.002557914	22.03395	7
AGTACTT	5590	0.0	21.60612	12-13
GTACTTT	5805	0.0	21.164616	14-15
ACGCAGA	12220	0.0	20.106434	7
CATGGGG	3860	0.0	19.929201	4
CGCAGAG	12270	0.0	19.878487	8
ACTGCGC	270	1.1078537E-6	19.830004	8
CAGTGCG	310	1.9555773E-7	19.191933	9
AGTCCGT	195	9.922463E-4	18.304619	8
GCAGAGT	12655	0.0	18.288069	9
ACTTTTT	7280	0.0	18.102598	16-17
>>END_MODULE