FastQCFastQC Report
Mon 15 Aug 2016
SRR2921138_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921138_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768102
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25170.14235604054517215No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23750.13432482967611598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74900.031.5553461
CATGGGG61800.029.8497624
GTACATG134300.027.5283281
TACATGG131500.027.0632482
TATCAAC90300.026.1641862
TCAACGC90700.025.587334
CAACGCA90450.025.4592425
ACATGGG136450.025.2964133
AACGCAG91450.025.2444866
GGTATCA56600.025.1388661
ATCAACG93400.024.913483
GTATAGG4700.022.800211
ACGCAGA102600.022.0951067
TACTAGC4900.021.861552
ATGGGGA32400.021.4874045
CGCAGAG105400.021.3952358
GTAAGGT7250.021.3404224
GTGTAGC18350.020.4394261
ATGGGGG24200.020.1623695
CCCTATA3551.8280844E-920.1167322
Produced by FastQC (version 0.11.5)